Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Raggi, Maria Augusta; Cavrini, Vanni; Pietra, Anna Maria Di

doi:10.1002/jps.2600711218

Cited by 49 publications

(28 citation statements)

References 18 publications

(3 reference statements)

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“…In the literature, there have been reported many spetrophotometric methods for the determination of NAC. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] One group of described methods involves redox reactions with NAC in which a colored compound is formed or decomposed during determination. Among the described indirect spectrophotometric procedures one of the most common approaches to the determination of NAC is based on the coupled redox complexation reaction.…”

Section: Introductionmentioning

confidence: 99%

“…Among the described indirect spectrophotometric procedures one of the most common approaches to the determination of NAC is based on the coupled redox complexation reaction. The first step is the reduction of Fe(III) by NAC, followed by the determination of produced Fe(II) using various chromogenic reagents: 1,10-phenantroline, [4,8,12] hexacyanoferrate(III), [13] ferrozine, [6] 2,4,6-trypyridyl-striazine [9,18] and ferricyanide. [14] Cu(II)-neocuproine has also been used for the analysis of NAC of which the sulfhydryl group (SH) reduces Cu(II)-neocuproine to the chromogenic Cu(I)-neocuproine.…”

Section: Introductionmentioning

confidence: 99%

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Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kukoc-Modun¹,

Tsikas²,

Kraljević³

et al. 2017

Croat. Chem. Acta

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Three simple, sensitive and robust kinetic spectrophotometric methods for the determination of a novel lipophilic thiol compound, N-acetyl-L-cysteine ethyl ester (NACET), have been developed and validated. The methods are based on the reduction of Cu(II)-ligand complex to Cu(I)-ligand complex with the analyte. Studied ligands were neocuproine, bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The development of chromogenic complexes was followed using kinetic setup with spectrophotometric detection at 458, 562 and 483 nm for the reactions of NACET with neocuproine, BCA and BCS, respectively. The calculated reaction orders with respect to NACET concentration were found to be 1.07, 1.01, 1.07, respectively, thus confirming a first order of reaction. The initial rate and fixed time methods were utilized for constructing calibration curves. Assays limits of detection were 1.4 × 10 -7 , 3.2 × 10 −7 and 6.0 × 10 −8 mol L -1 , respectively. The analytical performance of the methods, in terms of accuracy and precision, was established.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kukoc-Modun¹,

Tsikas²,

Kraljević³

et al. 2017

Croat. Chem. Acta

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“…1 The European Pharmacopeia recommended iodimetric titration for pharmaceutical formulations analysis. 2 Other reported methods described in the literature comprise: titrimetry, 3,4 spectrophotometry, [5][6][7][8][9] fluorimetry, [10][11][12] chromatography, 13,14 potentiometry, 15 conductometry 16 and voltametry [17][18][19] for the determination of NAC in pure form, in dosage forms and in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Radić¹,

Kukoc-Modun²,

Biocic³

2013

Croat. Chem. Acta

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“…Intravenous and oral administrations of NAC have been extensively used in the management of paracetamol (acetaminophen) poisoning. 1 Several analytical methods have been developed for NAC determination in pharmaceutical preparations, including spectrophotometric, [2][3][4][5][6] fluorometric, 7,8 chromatographic 9,10 and electrochemical methods. 11,12 However, these methods are not sufficiently sensitive and selective, or some of them require expensive instrumentation and are too expensive for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Based on a Coupled Redox-Complexation Reaction

Kukoc-Modun

Radić

2010

ANAL. SCI.

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Surprisingly, to the authors' knowledge, there are only two published kinetic spectrophotometric methods for the determination of NAC. 15,16 Also, none of the cited methods for the determination of NAC has used Fe 3+ and 2,4,6-trypyridyl-s-triazine (TPTZ) as a reagent solution. In this report, a novel, simple and sensitive kinetic spectrophotometric method with TPTZ as the chromogenic reagent for the determination of NAC is described and validated. Ease of application, sensitivity, short analysis time, low cost and reliability are its main advantages. The initial rate and fixed-time methods, after their optimization and validation, are adopted for the determination of NAC in its pharmaceutical formulations. Experimental ReagentsAll chemicals were of analytical-reagent grade, and solutions were prepared in Milli-Q deionized water.A stock solution of NAC, 1.0 × 10 -2 mol L -1 , was prepared by dissolving 163.2 mg of NAC (Merck, Darmstadt, Germany) in deionized water up to 100.0 mL in volume. The stock solution was stable for at least 30 days when it was stored in a dark bottle at 4 C. Working solutions of lower concentration were prepared daily by appropriate dilution of the stock solution with deionized water.A stock solution of Fe 3+ , 1.0 × 10 -2 mol L -1 , was prepared by dissolving 270.3 mg of FeCl3·6H2O (Kemika, Zagreb, Croatia) in a portion of deionized water. Concentrated hydrochloric acid (0.5 mL) was added to prevent the hydrolysis of iron, before making up to a volume of 100.0 mL.A stock solution of TPTZ (Merck, Germany), 1.0 × 10 -2 mol L -1 , was prepared by dissolving 312.3 mg in 2.0 mL of HCl (c (HCl) = 6.0 mol L -1 ), and diluted to 100.0 mL with deionized water. A stock solution of TPTZ was stored in a dark bottle at 4 C. A novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on a coupled redox-complexation reaction, the first step of which is the reduction of Fe 3+ by NAC; the second one includes the complexation of Fe 2+ , resulting from the preceding redox reaction, with 2,4,6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)2 2+ complex exhibits an absorption maximum at λ = 593 nm. The initial rate and fixed-time (at 5 min) methods were utilized for constructing calibration graphs. The graphs were linear in concentration ranges from 4.0 × 10 -6 to 1.0 × 10 -4 mol L -1 for the initial rate method and 1.0 × 10 -6 to 1.0 × 10 -4 mol L -1 for the fixed-time method, with detection limits of 1.0 × 10 -6 and 1.7 × 10 -7 mol L -1 , respectively. The proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations.

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Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Cited by 49 publications

References 18 publications

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)Ln Complexes with Different Ligands

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Based on a Coupled Redox-Complexation Reaction

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