Spectrophotometric assay of creatinine in human serum sample

Avinash, Krishnegowda; Nagaraja, Padmarajaiah; Shivakumar, Anantharaman; Krishna, Honnur

doi:10.1016/j.arabjc.2013.07.030

Cited by 33 publications

(13 citation statements)

References 19 publications

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“…Hematological analysis of blood was performed to determine white blood cell (WBC) count. UV/visible colorimetric analysis was used to determine plasma creatinine, blood urea nitrogen (BUN) and albumin levels, as previously described [ [18] , [19] , [20] ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of bone and kidney toxicity of BT2-peg2, a potential carrier for the targeted delivery of antibiotics to bone

et al. 2021

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Section: Methodsmentioning

confidence: 99%

Evaluation of bone and kidney toxicity of BT2-peg2, a potential carrier for the targeted delivery of antibiotics to bone

et al. 2021

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“…Creatinine filtration is performed in the kidney without reabsorption 6,16 . Therefore, all of the creatinine produced by the contraction of the muscles is extruded from the body through the urinary tract 17,18 . The serum concentration of creatinine is an important biomarker for kidney failure detection, glomerular filtration rate, and muscular dystrophy 13–15,19 …”

Section: Introductionmentioning

confidence: 99%

Trend in creatinine determining methods: Conventional methods to molecular‐based methods

Narimani

Esmaeili

Rasta

et al. 2020

Analytical Science Advances

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Renal failure (RF) disease is ranked as one of the most prevalent diseases with severe morbidity and mortality. Early diagnosis of RF leads to subsequent control of disease to reduce the poor prognosis. The level of sera creatinine is considered as a significant biomarker for kidney biofunction, which is routinely detected by the Jaffe reaction. The normal range for creatinine in the blood may be 0.84‐1.21 mg/dL. Low accuracy, insufficient sensitivity, explosive and toxicity of picric acid, and pseudo‐interaction with nonspecific elements such as ammonium ions in the Jaffe method lead to the development of various techniques for precise detection of creatinine such as spectroscopic, electrochemical, and chromatography approaches and sensors based on enzymes, molecular imprinted polymer and nanoparticles, etc. Based on previously established results, they are trying to construct sensors with high accuracy, optimum sensitivity, acceptable linear/calibration range, and limit of detection, which are small in size and applicable by the patient him/herself (point‐of‐care testing). By comparing the results of research, a molecularly imprinted electrochemiluminescence‐based sensor with linear/calibration range of 5‐1 mM0.33emconcentration of creatinine and the detection limit of 0.5 nM has the best detectable resolution with 2 million measurable points. In this paper, we will review the recently developed methods for measuring creatinine concentration and renal biofunction.

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“…Nowadays, numerous creatinine detection methods have been reported, such as high performance liquid chromatography (HPLC) [5], liquid chromatography–isotope dilution mass spectrometry (IDMS) [6], potentiometric [7] and amperometric creatinine biosensors [8], and so on [9,10,11,12,13,14,15]. Due to complicated sample processing and the requirement for experienced experts or instruments [16], the above analytical methods are unsuitable for quick detection, and most patients who need to repeat diagnosis multiple times or self-diagnosis, which greatly limits the practical application of these techniques.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Colorimetric Detection of Creatinine Based on Synergistic Effect of PEG/Hg2+–AuNPs

et al. 2019

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A colorimetric sensor, based on the synergistic coordination effect on a gold nanoparticle (AuNP) platform has been developed for the determination of creatinine. The sensor selects citrate stabilized AuNPs as a platform, polyethylene glycol (PEG) as a decorator, and Hg2+ as a linkage to form a colorimetric probe system (PEG/Hg2−–AuNPs). By forming hydrogen bond between the oxygen-containing functional groups of PEG and citrate ions on the surface of AuNPs, this probe shows good stability. PEG coordinated with Hg2+ synergistically and specifically on the surface of dispersed AuNPs, and the existence of creatinine could induce the aggregation of AuNPs with a corresponding color change and an obvious absorption peak shift within 5 min. This PEG/Hg2+–AuNPs probe towards creatinine shows high sensitivity, and a good linear relationship (R2 = 0.9948) was obtained between A620–522 nm and creatinine concentration, which can achieve the quantitative calculations of creatinine. The limit of detection (LOD) of this PEG/Hg2+–AuNPs probe was estimated to be 9.68 nM, lower than that of many other reported methods. Importantly, the sensitive probe can be successfully applied in a urine simulating fluid sample and a bovine serum sample. The unique synergistic coordination sensing mechanism applied in the designation of this probe further improves its high selectivity and specificity for the detection of creatinine. Thus, the proposed probe may give new inspirations for colorimetric detection of creatinine and other biomolecules.

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Spectrophotometric assay of creatinine in human serum sample

Cited by 33 publications

References 19 publications

Evaluation of bone and kidney toxicity of BT2-peg2, a potential carrier for the targeted delivery of antibiotics to bone

Evaluation of bone and kidney toxicity of BT2-peg2, a potential carrier for the targeted delivery of antibiotics to bone

Trend in creatinine determining methods: Conventional methods to molecular‐based methods

Highly Sensitive and Selective Colorimetric Detection of Creatinine Based on Synergistic Effect of PEG/Hg2+–AuNPs

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