Spectrophotometric and Stability–Indicating High–Performance Liquid Chromatographic Determinations of Terbutaline Sulfate

Abstract

Spectrophotometric and stability-indicating HPLC procedures are described for determination of terbutaline sulfate in bulk powder and dosage form. The first procedure is based on diazo coupling of the phenolic groups of terbutaline sulfate with fast red B salt in the presence of sodium hydroxide. The colored compound developed in alkaline medium was measured at 475 nm. Different variables affecting the reaction were studied. Beer's Law is obeyed in the concentration range of 1-6 microg/mL. In the HPLC procedure, the separation was carried out on a Caltrex AIII column, a relatively new packing material consisting of silica-bonded calix[8]arene, using an isocratic binary mobile phase, acetonitrile-ammonium acetate (50 + 50, v/v), at pH 6.2. A diode array detector was used at 280 nm. The method was validated for system suitability, linearity, precision, LOD, LOQ, specificity, stability, and robustness. The LOD and LOQ were 0.196 and 0.781 microg/mL, respectively. The recovery values of this method were between 98 and 102%, and the reproducibility was within 0.92%. Statistical comparison of the results obtained from the analysis of the studied drug to those of the official British Pharmacopoeia (2007) method using t- and F-tests showed no significant difference between them.

Online spectrophotometric determination of Fe(II) and Fe(III) by flow injection combined with low pressure ion chromatography

“…applied in Fe speciation, e.g. ICP-AES method [12], capillary electrophoresis with ultraviolet detection method [13], flame atomic absorption spectrometry [14], ion selective membrane potentiometric sensor [15], combination of sequential injection analysis and flame atomic absorption spectrometry [16,17], microcolumn ion chromatography with UV-Vis detection [18] as well as combination post-column derivatization HPLC or flow injection technique method and visible absorbance detection [3,13,16] were also reported. Regarding these spectrophotometric methods, iron has been determined using, e.g.…”

“…Regarding these spectrophotometric methods, iron has been determined using, e.g. phenanthroline [1], resorcylic acid [3], solochrome yellow 2GS [5], 8-hydoxyquinoline [6], di-2-pyridyl ketone benzoylhydrazone (DPKBH) [7], 1,10-phenanthroline [13], 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine (PDT) [10] or cetylpyridinium chloride [8] as reagents. Some of the proposed methods are based on determination of one form of the iron and/or the total iron, concentration of the other analyte is then calculated by subtracting the concentration of the concentration found for the total iron.…”

“…UV/Vis spectrophotometry [1][2][3][4][5][6][7][8][9][10][11] is the most common analytical method applied to Fe speciation. Besides, the other techniques http://dx.doi.org/10.1016/j.saa.2014.…”

Green Spectrophotometric Estimation of Minor Concentrations of Methyldopa and Terbutaline Sulphate in Pure Forms and Tablets Using Polyvinylpyrrolidone-Capped Silver Nanoparticles

“…It is also indicated for premature labour because of decreasing uterine contractility [2]. From the literature survey, TER was estimated in different dosage forms using spectrophotometric [17][18][19][20][21], spectrofluorometric [19,22] and electrochemical methods [23,24]. Chromatographic analysis of TER were performed using HPTLC [25], gas chromatography [26] as well as HPLC [20] in biological fluids [27] and in combined dosage forms [28] with stability indicating studies [29].…”

Chromatographic analysis of some drugs employed in erectile dysfunction therapy: Qualitative and quantitative studies using calixarene stationary phase

“…Several techniques such as UV , NMR , electrochemical , chromatographic , and capillary electrophoretic techniques were used to ensure that calixarenes make inclusion complexes with different analytes. Calixarenes have several applications in anion receptors , CE as additives , polymeric materials , GC , and HPLC .…”