Spectrophotometric and electrochemical determination of the formation constants of the complexes Curcumin–Fe(III)–water and Curcumin–Fe(II)–water

Bernabé-Pineda, Marganta; Ramı́rez-Silva, Maria Teresa; Romero-Romo, M.; González‐Vergara, Enrique; Rojas-Hernándéz, Alberto

doi:10.1016/s1386-1425(03)00344-5

Cited by 91 publications

(62 citation statements)

References 13 publications

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“…Curcumin is a polyphenol and complexes readily with a number of different metal ions. In aqueous solutions of neutral pH, curcumin is an effective chelator of Fe(III) (2). Curcumin is also lipophilic and readily crosses membranes (19), so therefore it may also chelate metal ions intracellularly.…”

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confidence: 99%

“…Chelators can deplete the exogenous pool of metal ions from the growth medium, enter cells and deplete the intracellular reserves of metal ions, or act both intra-and extracellularly to alter metal ion pools. Curcumin is able to bind iron in solution, demonstrating that it is an effective extracellular iron chelator (2). To determine if curcumin can also traverse the yeast cell wall and plasma membrane to act intracellularly, we exploited the natural fluorescent properties of curcuminoids.…”

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Curcumin Inhibits Growth of Saccharomyces cerevisiae through Iron Chelation

et al. 2011

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Curcumin, a polyphenol derived from turmeric, is an ancient therapeutic used in India for centuries to treat a wide array of ailments. Interest in curcumin has increased recently, with ongoing clinical trials exploring curcumin as an anticancer therapy and as a protectant against neurodegenerative diseases. In vitro, curcumin chelates metal ions. However, although diverse physiological effects have been documented for this compound, curcumin's mechanism of action on mammalian cells remains unclear. This study uses yeast as a model eukaryotic system to dissect the biological activity of curcumin. We found that yeast mutants lacking genes required for iron and copper homeostasis are hypersensitive to curcumin and that iron supplementation rescues this sensitivity. Curcumin penetrates yeast cells, concentrates in the endoplasmic reticulum (ER) membranes, and reduces the intracellular iron pool. Curcumin-treated, iron-starved cultures are enriched in unbudded cells, suggesting that the G 1 phase of the cell cycle is lengthened. A delay in cell cycle progression could, in part, explain the antitumorigenic properties associated with curcumin. We also demonstrate that curcumin causes a growth lag in cultured human cells that is remediated by the addition of exogenous iron. These findings suggest that curcumin-induced iron starvation is conserved from yeast to humans and underlies curcumin's medicinal properties.Curcumin is the major chemical component of turmeric, a dietary spice made from the root of the Curcuma longa Linn plant and used extensively in traditional Indian medicine (38). Curcumin is a potent bioactive compound that is used to treat cancer (5, 35), atherosclerosis (33), and neurodegenerative diseases, such as Alzheimer's (26, 45) and Parkinson's (44) disease, as well as to promote wound healing (15,36). Curcumin is particularly appealing as a therapeutic agent because of its extremely low toxicity. Many biological activities have been ascribed to curcumin. For example, curcumin suppresses inflammatory responses in cultured cells and in animals and also exhibits antioxidant properties. Furthermore, curcumin's ability to inhibit tumorigenesis and proliferation of a wide variety of cancerous cells has been well documented. Curcumin is a polyphenol and complexes readily with a number of different metal ions. In aqueous solutions of neutral pH, curcumin is an effective chelator of Fe(III) (2). Curcumin is also lipophilic and readily crosses membranes (19), so therefore it may also chelate metal ions intracellularly. How these chemical properties contribute to curcumin's biological activities, however, is not understood.Identifying relevant in vivo targets of small molecules is technically challenging. Recently, several genetic and genomic approaches have been developed that use the simple eukaryote Saccharomyces cerevisiae, or budding yeast, to study the mechanism of drug action (17,27,31). One such method, termed homozygous profiling, uses a comprehensive collection of 4,700 homozygous diploid deletion yeast...

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Curcumin Inhibits Growth of Saccharomyces cerevisiae through Iron Chelation

et al. 2011

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“…This suggested the possibility that curcumin bound strongly to Fe. The reaction between the complexes curcumin-Fe 2+ and curcumin-Fe 3+ studied in aqueous media using UV spectrophotometry and cyclic voltammetry also showed a similar behavior, and indicated that a chemical reaction had occurred between the curcumin and Fe before the formation of the complexes (Bernabé-Pineda et al, 2004). Verma and Goldin (2003) recently reported that in the presence of proteins Cu 2-ions may not react with curcumin to generate DNA damaging species, in contrast to the data obtained with a curcumin-Cu 2-combination in cell-free systems (Ahsan and Hadi, 1998).…”

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confidence: 72%

“…biological processes such as oxygen transfer and DNA synthesis (Bernabé-Pineda et al, 2004). Table 2 shows that neither Fe significantly protected CHO cells against curcumin-induced chromosomal damage in a concentration-dependent manner compared to treatment with curcumin alone.…”

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Effects of H2O2, Fe2+ and Fe3+ on curcumin-induced chromosomal aberrations in CHO cells

et al. 2005

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The effects of H 2 O 2 , Fe 2+ and Fe 3+ on curcumin-induced clastogenicity were evaluated in CHO cells. Curcumin combined with H 2 O 2 did not increase the chromosomal aberrations more than expected based on a simple additive effect. In contrast, the combination of curcumin-Fe significantly decreased the total number of chromosomal aberrations and the number of abnormal metaphases. The clastogenicity of curcumin may be related to its pro-oxidant properties and its ability to generate free radicals.

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“…The HPLC, GLC, potentiometric, and spectrophotometric methods were used for the studies of curcumin analysis 15,16 . However, the literature is silent on the use of polarographic methods for the analysis of curcumin in, different samples.…”

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Electrochemical Analysis of Natural Chemopreventive Agent (Curcumin) in Extracted Sample and Pharmaceutical Formulation

Modi¹,

Pitre²

2010

DSJ

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Curcumin has anti-oxidative and anticarcinogenic activities. This study shows the electrochemical behaviour of curcumin using polarography, i.e., DC polarography and differential pulse polarography (DPP) methods. In ammonium tartrate as supporting electrolyte, the differential pulse polarogram of curcumin shows two conjugated peaks with peak potential (E p ) 1125 mV and 1275 mV vs SCE. However, the direct current polarogram shows only one polarographic wave with E 1/2 which was 1275 mV. The developed electrochemical methods have been standardised for the determination of curcumin in extracted sample of natural origin and its pharmaceutical formulation. The electrochemical analysis has been supplemented by ultraviolet and infrared spectral analyses of the samples.

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Spectrophotometric and electrochemical determination of the formation constants of the complexes Curcumin–Fe(III)–water and Curcumin–Fe(II)–water

Cited by 91 publications

References 13 publications

Curcumin Inhibits Growth of Saccharomyces cerevisiae through Iron Chelation

Curcumin Inhibits Growth of Saccharomyces cerevisiae through Iron Chelation

Effects of H2O2, Fe2+ and Fe3+ on curcumin-induced chromosomal aberrations in CHO cells

Electrochemical Analysis of Natural Chemopreventive Agent (Curcumin) in Extracted Sample and Pharmaceutical Formulation

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