Electrochemical Analysis of Natural Chemopreventive Agent (Curcumin) in Extracted Sample and Pharmaceutical Formulation

Abstract

Curcumin has anti-oxidative and anticarcinogenic activities. This study shows the electrochemical behaviour of curcumin using polarography, i.e., DC polarography and differential pulse polarography (DPP) methods. In ammonium tartrate as supporting electrolyte, the differential pulse polarogram of curcumin shows two conjugated peaks with peak potential (E p ) 1125 mV and 1275 mV vs SCE. However, the direct current polarogram shows only one polarographic wave with E 1/2 which was 1275 mV. The developed electrochemical methods have been standardised for the determination of curcumin in extracted sample of natural origin and its pharmaceutical formulation. The electrochemical analysis has been supplemented by ultraviolet and infrared spectral analyses of the samples.

A Newly Developed Electrocatalytic Oxidation and Voltammetric Determination of Curcumin at the Surface of PdNp-graphite Electrode by an Aqueous Solution Process with Al3+

“…Limit of detection (LOD) and limit of quantification (LOQ) were found to be 2.2×10 -8 M and 6.7×10 -8 M, respectively. The determination parameters of curcumin for this assay were compared with those of other studies, [38][39][40] and the results were presented in Table 2. As can be seen from Table 2, the novel developed Al 3+ /PdNp/GE can provide an assay with appropriate concentration range and lower LOD.…”

Electrochemical Sensing of Curcumin: A Review

“…Curcumin electrochemical behavior was also investigated using direct current (DC) polarography and differential pulse polarography (DPP) in ammonium tartrate aqueous solutions, at pH = 8.1. DPP showed two conjugated reduction peaks, at E p = −1.12 V and E p = −1.28 V, vs. SCE, while DC polarography showed only one wave, at E 1/2 = −1.28 V, vs. SCE [17]. Using an Al 3+ /Pd nanoparticles (NPs)-modified graphite electrode (GE) in phosphate buffer saline solution at pH = 2, the curcumin oxidation showed one anodic peak, at E p = +0.56 V, and two cathodic peaks, at E p = +0.50 V and E p = +0.30 V, vs. Ag/AgCl (3 M KCl) [18].…”

Curcumin Electrochemistry—Antioxidant Activity Assessment, Voltammetric Behavior and Quantitative Determination, Applications as Electrode Modifier

“…In the direct current and differential pulse polarographic curves, these processes generated a signal with the half-wave potential of −1.275 V and two cathodic peaks with the potentials E pc1 = −1.125 V and E pc2 = −1.275 V versus SCE, respectively. Due to its higher sensitivity, DPP was applied to quantify CU in turmeric powder and pharmaceutical formulations [82].…”

“…In the direct current and differential pulse polarographic curves, these processes generated a signal with the half-wave potential of −1.275 V and two cathodic peaks with the potentials Epc1 = −1.125 V and Epc2 = −1.275 V versus SCE, respectively. Due to its higher sensitivity, DPP was applied to quantify CU in turmeric powder and pharmaceutical formulations [82]. A CV investigation carried out at HMDE in BRB solution containing 1% ethanol pointed out that, in acidic medium (pH 6.00), CU presented two irreversible pH dependent cathodic peaks (Epc1 = ~−1.000 V and Epc2 = ~−1.200 V versus Ag/AgCl) generated by the reduction of the diketone group (Figure 3b), while, in a basic environment (pH 9.50), only one peak (Epc = ~−1.100 V versus Ag/AgCl) was observed.…”