Spectrophotometric and chemometric determination of hydrochlorothiazide and spironolactone in binary mixture in the presence of their impurities and degradants

Abstract

Hydrochlorothiazide (HCT) and spironolactone (SPR) are mostly co-formulated in antihypertensive formulations. Several methods have been developed and validated for their determination; these methods include spectrophotometric and chemometric-assisted spectrophotometric methods. The developed spectrophotometric methods were isosbestic point (ISO) and ratio subtraction (RS) methods. The absorbance values at 232.4 (lambda(iso1)) and 257.6 nm (lambda(iso2)) were used for determination of the total mixture concentration, while HCT could be directly determined at 317.2 nm (lambda(max)) and by subtraction SPR concentration could be obtained. Also SPR concentration could be calculated by RS method using the absorbance at 243.8 nm (lambda(max)). A wavelength selection method based on genetic algorithm (GAs) was developed and compared to the conventional partial least squares method (PLS). In this method, several parameters were adjusted and the optimum parameter settings were determined using experimental design. The developed chemometric methods were successfully applied for the determination of the HCT and SPR, as well as for determination of their impurities and degradation products. The proposed methods were successfully applied for determination of HCT and SPR in commercial tablets and they were statistically compared to each other and to the reported method. No significant difference was found, providing their accuracy and precision.

Pharmaceuticals and Related Drugs

“…Besides the expanding number of vibrational methods, many UV and NMR methods also have appeared in the literature for carrying out various pharmaceutical measurements. Examples of some of these are summarized in Table . − In one instance, a surface plasmon resonance sensor (PRS) was developed to quantify dextromethorphan in enzyme containing solutions . The PRS is based on an optical fiber bundle with its sensing surface coated with a thin layer of molecularly imprinted β-cyclodextrin polymer.…”

Overview of the Determination of Hydrochlorothiazide Levels in Pharmaceutical Preparations and Biological Matrices

“…The proposed method was successfully applied to determine HCT and SPR in commercial tablets and statistically compared with each other and with the methods reported. No significant differences were found, giving accuracy and precision [15]. Methods for determining hydrochlorothiazide (HCT) and spironolactone (SPR) in their mixtures and the presence of impurities or their degradation products have been developed.…”

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

“…Spectroscopic [8][9][10], High Performance Liquid Chromatography (HPLC) [11][12][13], Thin Layer Chromatography (TLC) [14], Electrochemical [15][16][17] and mass spectrometry [18] methods for estimation of MOX alone or in combination with other drugs in pharmaceutical preparation have been reported. Hydrochlorothiazide alone or in combination with other drugs is estimated by HPLC [19][20][21][22][23][24][25][26][27][28][29], TLC-densitometry [30][31][32][33][34][35][36], capillary electrophoresis [37][38][39], electrochemical [40][41][42][43] and spectroscopic methods [44][45][46][47][48][49][50][51][52][53]. Simultaneous estimation of MOX and HCT has been reported by RP-HPLC [54,…”