Spectrophotometric Analysis of Nucleic Acids

Heptinstall, John; Rapley, Ralph

doi:10.1385/1-59259-038-1:57

Cited by 13 publications

(9 citation statements)

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“…Genomic DNA was extracted from young leaves using the CTAB method (Doyle and Doyle, 1987) with some modifications. DNA quantitation was performed with a Nanodrop ND-1000 (Nanodrop Technologies an indication of DNA quality (Heptinstall and Rapley, 2000). In order to analyze DNA integrity we performed an electrophoresis in agarose gel at 1% and staining with ethidium bromide (0.8 mg/ ml) and the bands were visualized with ultraviolet light in a transilluminator.…”

Section: Methodsmentioning

confidence: 99%

Increasing the Genetic Base of Modern Cultivars of Eggplant of the Semi-Long Black Type

Hurtado¹,

Vilanova²,

Gramazio³

et al. 2015

BUASVMCN-HORT

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The eggplant (Solanum melongena) semi-long black varietal type is the most important in most European markets. Although open pollinated, pure lines and F1 hybrid cultivars exist within this varietal group, the latter predominate in the commercial production of eggplants, especially under greenhouse conditions. However, molecular markers studies have found that modern F1 hybrids present a reduced genetic base. This work is aimed at using a wide diversity of black eggplants for developing pure lines for obtaining hybrids heterotic for yield and increasing the genetic base of the black eggplants genepool. Thirty hybrid and non-hybrids varieties of black eggplants were used. Materials were characterized for morphological and agronomic traits of interest under greenhouse conditions. Molecular characterization was also performed using 16 SSR markers. A pedigree breeding programme was performed based on morphoagronomic traits until the F8 or F9 generations. Molecular analysis revealed that original materials could be separated in three main clusters groups, one made up by varieties from large companies that include the most successful varieties and the two other by an admixture of materials including non-hybrid varieties and F1 hybrids with moderate or low economic importance. The pedigree selection made allowed the final selection of 15 lines, which according to its origin, should have an increased genetic diversity compared to modern F1 hybrids. These lines, which present a very good performance under greenhouse conditions have been crossed in order to obtain hybrids heterotic for yield. These selected lines will be used to obtain a new generation of eggplant F1 hybrids with increased genetic base.

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Section: Methodsmentioning

confidence: 99%

Increasing the Genetic Base of Modern Cultivars of Eggplant of the Semi-Long Black Type

Hurtado¹,

Vilanova²,

Gramazio³

et al. 2015

BUASVMCN-HORT

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“…The OD260/280 ratio was used to determine DNA purity and concentration. 91 Values of >1.7 were accepted.…”

Section: Methodsmentioning

confidence: 99%

Radiolabeled 5-Iodo-3′-O-(17β-succinyl-5α-androstan-3-one)-2′-deoxyuridine and Its 5′-Monophosphate for Imaging and Therapy of Androgen Receptor-Positive Cancers: Synthesis and Biological Evaluation

Kortylewicz

Nearman

Baranowska‐Kortylewicz

2009

J. Med. Chem.

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High levels of androgen receptor (AR) are often indicative of recurrent, advanced, or metastatic cancers. These conditions are also characterized by a high proliferative fraction. 5-Radioiodo-3′-O-(17β-succinyl-5α-androstan-3-one)-2′-deoxyuridine 8 and 5-radioiodo-3′-O-(17β-succinyl-5α-androstan-3-one)-2′-deoxyuridin-5′-yl monophosphate 13 target AR. They are also degraded intracellularly to 5-radioiodo-2′-deoxyuridine 1 and its monophosphate 20, respectively, which can participate in the DNA synthesis. Both drugs were prepared at the no-carrier-added level. Precursors and methods are readily adaptable to radiolabeling with various radiohalides suitable for SPECT and PET imaging, as well as endoradiotherapy. In vitro and in vivo studies confirm the AR-dependent interactions. Both drugs bind to sex hormone binding globulin. This binding significantly improves their stability in serum. Biodistribution and imaging studies show preferential uptake and retention of 8 and 13 in ip xenografts of human ovarian adenocarcinoma cells NIH:OVCAR-3, which overexpress AR. When these drugs are administered at therapeutic dose levels, a significant tumor growth arrest is observed.

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“…29 The method is relatively accurate, reproducible, and fast when applied to moderately diluted or concentrated pure preparations (0.5 -15.0 μ g/mL). 29 The method is relatively accurate, reproducible, and fast when applied to moderately diluted or concentrated pure preparations (0.5 -15.0 μ g/mL).…”

Section: Spectrophotometrymentioning

confidence: 99%

Product Specifications and Quality Control

Prazeres¹

2011

Plasmid Biopharmaceuticals

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Spectrophotometric Analysis of Nucleic Acids

Cited by 13 publications

References 0 publications

Increasing the Genetic Base of Modern Cultivars of Eggplant of the Semi-Long Black Type

Increasing the Genetic Base of Modern Cultivars of Eggplant of the Semi-Long Black Type

Radiolabeled 5-Iodo-3′-O-(17β-succinyl-5α-androstan-3-one)-2′-deoxyuridine and Its 5′-Monophosphate for Imaging and Therapy of Androgen Receptor-Positive Cancers: Synthesis and Biological Evaluation

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