Spectrometric-based techniques for metal-binding protein assessment in clinical, environmental, and food samples

Chantada-Vázquez, María Del Pilar; Moreda-Piñeiro, Antonio; Barciela‐Alonso, María Carmen; Bermejo-Barrera, Pilar

doi:10.1080/05704928.2016.1213736

Cited by 14 publications

(9 citation statements)

References 126 publications

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“…In procedure 3, retention times were calibrated using a Low Molecular Weight Gel Filtration Calibration Kit (GE Healthcare, Marlborough, MA, USA) consisting of five proteins with MW in the range 6500 to 75,000 Da. Retention times were determined using a UV/VIS detector in place of ICP-DRC-MS [ 35 , 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, electrospray ionization mass spectrometry (ESI-MS) seems to be a perfect tool for the determination of the structures of organic compounds potentially present in drinking water samples. In the literature, one can find various applications of ESI-MS in the analysis of organic compounds containing metal atoms, and also arsenic, in food samples [ 34 , 35 ]. New analytical capabilities can be created through the use of both the aforementioned analytical techniques, HPLC/ICP-MS and ESI-MS, in the analysis of a single sample.…”

Section: Introductionmentioning

confidence: 99%

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Study on Speciation of As, Cr, and Sb in Bottled Flavored Drinking Water Samples Using Advanced Analytical Techniques IEC/SEC-HPLC/ICP-DRC-MS and ESI-MS/MS

et al. 2019

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The main aim of the research was to develop a complementary analytical approach consisting of bespoke speciation analysis and non-targeted speciation analysis of As, Sb, and Cr in flavored bottled drinking water samples using HPLC/ICP-DRC-MS and ESI-MS/MS. The scope of two previously developed analytical procedures, (1) multielemental speciation procedure for AsIII, AsV, CrVI, SbIII, and SbV analysis and (2) arsenic speciation procedure for AsB, AsIII, DMA, MMA, and AsV quantification, was extended to the analysis of a new sample type in terms of bespoke speciation analysis. As for the non-targeted speciation, analysis size exclusion chromatography was used with ICP-MS and a complementary technique, ESI-MS/MS, was used for the organic species of As, Sb, and Cr screening. Full validation of procedures 1 and 2 was conducted. Procedure 1 and 2 were characterized with precision values in the range from 2.5% to 5.5% and from 3.6% to 7.2%, respectively. Obtained recoveries ranged from 97% to 106% and from 99% to 106% for procedures 1 and 2, respectively. Expanded uncertainties calculated for procedures 1 and 2 ranged from 6.1% to 9.4% and from 7.4% to 9.9%, respectively. The applicability of the proposed procedures was tested on bottled drinking water samples. Results for the real samples in procedure 1 were in the range from 0.286 ± 0.027 [μg L−1] to 0.414 ± 0.039 [μg L−1] for AsIII, from 0.900 ± 0.083 [μg L−1] to 3.26 ± 0.30 [μg L−1] for AsV, and from 0.201 ± 0.012 [μg L−1] to 0.524 ± 0.032 [μg L−1] for SbV. CrVI and SbIII were not detected in any sample. As for procedure 2, results were in the range from 0.0541 ± 0.0053 [μg L−1] to 0.554 ± 0.054 [μg L−1] for AsB. Results for AsIII and AsV obtained with procedure 2 were in good accordance with results obtained with procedure 1. DMA and MMA were not detected in any sample.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study on Speciation of As, Cr, and Sb in Bottled Flavored Drinking Water Samples Using Advanced Analytical Techniques IEC/SEC-HPLC/ICP-DRC-MS and ESI-MS/MS

et al. 2019

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“…31 The decrease of ash content could be due to the high temperatures used during the extrusion that alter the conformational structure of the macromolecules, especially in the proteins, generating destabilization and rupture of existing connections between the micronutrients, like the minerals, and these molecules, resulting in the loss of such components during the process. 32 The values observed for the extruded samples of both genotypes, SC 319 and BRS 332, are in agreement with other studies, such as those from Arbex et al 33 and Lopes et al 34 where sorghum grains were extruded in relation to carbohydrates (71.04 and 58.16%), proteins (11.26 and 12.20%), lipids (0.41 and 2.30%), ashes (1.87 and 1.38%) and fibers (8.84 and 14.59%), and the differences were attributed to the different genotypes of the studies and climatic conditions that interfere with nutrient deposition. 35 The nutritional characteristics of the evaluated sorghum genotypes resemble the most traditional cereals, like corn, wheat and rice.…”

Section: Centesimal Compositionmentioning

confidence: 99%

Study of Thermoplastic Extrusion and Its Impact on the Chemical and Nutritional Characteristics and Two Sorghum Genotypes SC 319 and BRS 332

Campelo¹,

Henriques²,

Simeone³

et al. 2020

J. Braz. Chem. Soc.

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The use of sorghum in human nutrition has been expanded due to its nutritional composition and its functional potential. Extrusion has been used to improve the quality of sorghum. The aim of this work was to assess the effect of extrusion in the nutritional composition, phenolic compounds content of two sorghum, and characterize the profile of chemical constituents by paper spray mass spectrometry (PS-MS). Extrusion increased the carbohydrate and fiber contents and reduced moisture, lipids and ashes, and enabling a higher degree of liberation of phenolic compounds and antioxidant activity. The fingerprint obtained in both ionization modes had been influenced by extrusion, allowing for a greater compounds' identification on the extruded samples. The analysis of sorghum samples' main components made distinguishing of the main constituents possible, as well as highlighted the extrusion effect and the influence of the genotypes. This study demonstrated that extrusion enabling a higher liberation of phenolic compounds, which were identified by the PS-MS technique.

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“…While it is logistically challenging to conduct such studies in whole animals, metallomics tools can be conveniently applied to probe the fate of novel metal-based drugs in human plasma in vitro [34], which makes the aforementioned proposition quite attractive today. Over the past 15 years a large variety of liquid chromatography-based metallomics approaches have been systematically developed to facilitate the analysis of complex biological fluids for endogenous metalloproteins [35][36][37]. Unsurprisingly, this conceptual bioanalytical approach was eventually employed to gain insight into the comparative biochemical fate of Pt-based anticancer drugs in human blood plasma in vitro [38].…”

Section: Application Of Metallomics To Probe the Interaction Of Cisplatin With Hsa In Blood Plasmamentioning

confidence: 99%

The cisplatin/serum albumin system: A reappraisal

Massai

Pratesi

Gailer

et al. 2019

Inorganica Chimica Acta

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Since the first approval of cisplatin for cancer treatment in 1978, a lot of attempts have been carried out to characterize in detail its interactions with serum albumin, by far the most important and most abundant plasma protein. The state of the art of those studies was recapitulated by Keppler and coworkers in an extensive review article which appeared in Chem. Rev. in 2006. Yet, the general picture was still rather incomplete at that time due to the lack of crystallographic data. Here, we report on the main achievements obtained on this system in the period 2006-2018 and try to describe what is now clearly ascertained and what are the still open issues. Remarkably, a detailed structural characterization of this metallodrug/protein system was recently obtained thanks to the resolution of the crystal structure of a cisplatin/serum albumin adduct; crystallographic results were nicely complemented by independent MS studies. In turn, metallomics investigations permitted to monitor platination of this serum protein in real blood samples. Thus, a rather complete description of the system was gained. In addition, the biological profile of cisplatin/serum albumin adducts was drafted in a specific study and its pharmacological implications discussed. The relevance and the impact of these novel results are herein critically analyzed.

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Spectrometric-based techniques for metal-binding protein assessment in clinical, environmental, and food samples

Cited by 14 publications

References 126 publications

Study on Speciation of As, Cr, and Sb in Bottled Flavored Drinking Water Samples Using Advanced Analytical Techniques IEC/SEC-HPLC/ICP-DRC-MS and ESI-MS/MS

Study on Speciation of As, Cr, and Sb in Bottled Flavored Drinking Water Samples Using Advanced Analytical Techniques IEC/SEC-HPLC/ICP-DRC-MS and ESI-MS/MS

Study of Thermoplastic Extrusion and Its Impact on the Chemical and Nutritional Characteristics and Two Sorghum Genotypes SC 319 and BRS 332

The cisplatin/serum albumin system: A reappraisal

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