Spectrofluorometric Resolution of Closely Overlapping Drug Mixtures Using Chemometrics Methods

Abstract

A modified parallel factors analysis (PARAFAC) algorithm with a penalty diagonalization error (PDE), newly proposed by the present authors, was utilized to simultaneously resolve drug mixtures of propranolol (PRO), dipyridamole (DIP) and amiloride (AMI) without any loss of sensitivity. The analyses were performed in aqueous solution. The experimental results demonstrated that the profiles of the spectra and the concentrations could be accurately resolved using the PDE algorithm with a high sensitivity and stable repeatability. That is to say, the closely overlapping problem of the spectra could be easily solved. Furthermore, simultaneous determinations of three kinds of tablets, which contain PRO, AMI and DIP, respectively, were successfully performed with satisfactory results.

Post‐modified non‐negative matrix factorization for deconvoluting the gene expression profiles of specific cell types from heterogeneous clinical samples based on RNA‐sequencing data

“…Therefore, there is a great need for the researchers to develop in silico methods to deconvolute the gene expression profiles of a heterogeneous clinical sample into the cell type‐specific gene expression profiles and estimate the corresponding cell contents . The multivariate curve resolution algorithms have achieved great success in quantitative analysis in chemistry and in related areas such as environmental science, food science, and pharmaceutical analysis . Several computational approaches based on the multivariate curve resolution algorithms have been proposed to deconvolute the gene expression profiles of specific cell types from the mixed signals generated by DNA microarrays.…”

Assessment of ion transfer amperometry at liquid–liquid interfaces for detection in CE

“…These drugs are b-blockers used in the treatment of heart disease and in eye drop formulations to treat elevated intraocular pressure associated with glaucoma [32,33]. They have also been classified by the International Olympic Committee as doping substances and are listed as forbidden pharmaceutical substances [34].…”

Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration

“…In other words, compared to other traditional measurement techniques, these methods can offer a highly attractive property, called "secondorder advantage", 27,28 which allows for the direct concentration determination of a single target component or simultaneous concentration determinations of multiple target components in complicated samples, even with uncalibrated interferences. The property has been a hotspot in the current chemometric domain, and successfully employed for many practical applications, including cosmetics analysis, 19 environment monitoring, 29-31 drugs analysis, 23,[32][33][34][35] quality control and food analysis. 36,37 For example, Rodriguez-Cuesta et al 30 simultaneously determined three pesticides of carbendazim, fuberidazole and thiabendazole by excitation-emission matrix fluorescence in combination with the PARAFAC algorithm.…”