Spectrofluorometric determination of piroxicam

Damiani, Patricia C.; Bearzotti, Mariela; Cabezón, Miguel A.; Olivieri, Alejandro C.

doi:10.1016/s0731-7085(97)00166-0

Cited by 49 publications

(15 citation statements)

References 16 publications

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“…Nos últimos anos, muitos grupos de pesquisas têm se dedicado ao desenvolvimento de novos métodos analíticos baseados na emissão de luminescência, dentre eles a quimilumi-nescência 16 A fluorescência nativa tem sido explorada para a quantificação de fármacos em medicamentos; com base nisto, muitos trabalhos têm sido descritos na literatura. 19,20,[58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74]80,[110][111][112][113][114][115][118][119][120][121][122]124,125,129,[133][134][135] A Tabela 1 mostra os principais fármacos determinados através da fluorescência nativa em solução.…”

Section: Aplicação Da Espectroscopia De Luminescência No Campo Farmacunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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Recebido em 20/10/06; aceito em 30/11/07; publicado na web em 4/9/08 APPLICATION AND ADVANCES IN THE LUMINESCENCE SPECTROSCOPY IN PHARMACEUTICAL ANALYSES. This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo-and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.Keywords: luminescence spectroscopy; drugs; cosmetics analysis; introdução Devido ao progresso contínuo em técnicas e instrumentos, a indústria farmacêutica busca o emprego de metodologias que ofereçam maior sensibilidade, seletividade, robustez, precisão, exatidão e rapidez. Neste sentido, um grande número de equipamentos de cromatografia líquida de alta eficiência (CLAE) com diversos tipos de detecção domina este ramo industrial. Contudo, o advento da sofisticação de instrumentação automatizada de espectrômetros de luminescência poderá resultar em um substancial aumento de aplicações desta técnica instrumental na indústria farmacêutica, principalmente, no que se refere à análise diretamente na matriz do medicamento.A espectroscopia de luminescência é uma ferramenta analítica extremamente sensível e tem sido amplamente aplicada na resolução de problemas que requerem baixos limites de detecção, podendo ser facilmente encontrados na literatura muitos trabalhos mostrando análises ao nível de traços.1-6 A sensibilidade inerente a esta técnica é consideravelmente maior em comparação a outras metodologias, entre elas a espectrofotometria UV/visível, apresentando limites de detecção de até três ordens de grandeza menores, os quais normalmente se encontram na faixa de ng mL -1 , sendo conseqüência do baixo sinal de fundo que as medidas fluorescentes apresentam. Diferentemente do que ocorre no fenômeno da luminescência, nas medidas de absorbância o sinal está associado à atenuação da intensidade de um feixe de radiação por uma espécie absorvente. Nos casos onde a quantidade da espécie é pequena, esta atenuação é menor, tornando assim a detecção da espécie difícil. Outra característica importante da luminescência diz respeito à seletividade, porque espécies com rendimentos quânticos de fluorescência apreciáveis são menos comuns do que substâncias cuja fluorescência não pode ser detectada. Desta forma, moléculas fluorescentes apresentam comprimento de onda característico de excitação e/ou emissão. Adicionalmente, o comprimento de onda de excitação d...

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Section: Aplicação Da Espectroscopia De Luminescência No Campo Farmacunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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“…Several methods have been reported for the assay of NSAIDs in pharmaceuticals and biological fluids; in general these works deal with a single compound or a few compounds, and employ capillary electrophoresis [10][11][12][13][14][15], spectrofluorimetry [16][17][18][19][20], flow injection fluorimetry [21] or gas chromatography coupled to mass spectrometry (GC/MS) [22][23][24]. GC methods are timeconsuming and have been largely replaced by HPLC methods with ultra-violet (UV) [25][26][27][28][29][30][31][32][33][34][35] detection, electrochemical detection [36], or mass spectrometry [37].…”

Section: Introductionmentioning

confidence: 99%

Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection

Ibrahim

Boyer

Bouajila

et al. 2007

Journal of Chromatography B

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“…20 The literature reports several analytical methods for the determination of PX in pharmaceutical samples and biological fluids [21][22][23][24][25][26] including spectrofluorimetry methods due to high sensitivity and selectivity. [27][28][29][30] In pharmaceutical preparations, PX is frequently combined with some vitamins of the B complex. Among B complex vitamins, pyridoxine, PY, (2-methyl-3-hydroxy-4,5-bis(hydroxyl-methyl)-pyridine (Scheme 1)) is one of the members of the vitamin B6 group, and possesses a significant native fluorescence in solution, which often interferes in the spectrofluorimetric determination of PX.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

2006

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One of the most serious problems that can occur in classical quantitative analysis is the presence of one or more spectral interferent-chemical species which affect the instrument response and which are unaccounted for in the calibration process. Advances in chemometric methods allow quantitative analysis in the presence of unidentified interferents if a threeway experimental data matrix is available for each sample. This property is the so-called "second-order advantage" 1 and it is based on the earlier work done in the psychometrics field. 2,3 The use of this advantage in analytical chemistry was proposed by Ho et al. 4 (rank annihilation factor analysis, RAFA), for the multicomponent analysis of fluorescent mixtures using excitation-emission matrix (EEM). Later, Lorber 5 and Sanchez and Kowalski 6 proposed new and simple solutions for the method of Ho et al. and now it is called the generalized rank annihilation method (GRAM).At present, analytical chemistry laboratories have instrumentation that easily generates multidimensional data structures of experimental data for each sample. Fluorescence is a particularly interesting technique because it allows, in a very straightforward manner, the acquisition of this type of information, of which the most typical example is the excitation-emission matrix (EEM). Several algorithms that use second-order data are described in the literature. [7][8][9] Of these, the generalized rank annihilation method (GRAM) 6,11,12 has interesting properties since it only requires two data matrices for quantification: one from the calibration sample and the other from the unknown sample. Recently, it has been proved that GRAM predicts results that are similar to those from the parallel factor analysis (PARAFAC) when only two samples are used, 9 so both methods can be useful as calibration methods for second-order data. But, unlike PARAFAC, GRAM is a non-iterative method which tries to implement the second-order advantage. Second-order calibration using GRAM has several distinct advantages. 13The most pronounced advantage is that it makes possible the quantification of the analyte(s) of interest in samples containing interferents. Those interferents can be completely unknown, that is, it is not necessary that they have been incorporated in the standards used for calibration. Another advantage is that it is possible to recover the individual instrument responses of the analyte(s) of interest, e.g., the excitation and emission spectra can be obtained for the individual analyte(s). This makes it possible to perform qualitative analysis and to check the identity of the analytes. The Kowalski group has proposed the trilinear decomposition (TLD) method. 14,15 In this work we focused our attention on the ability of GRAM for analyzing the second-order data.Fluorescence spectroscopy is a versatile tool mainly used because of its selectivity and sensitivity. Fluorescence spectra can be recorded in difference modes such as emission, excitation, synchronous and excitation-emission matrix (EEM). T...

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Spectrofluorometric determination of piroxicam

Cited by 49 publications

References 16 publications

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

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