Spectrofluorimetric Protocol for Ceftriaxone in Commercial Formulation and Human Plasma After Condensation with Formaldehyde and Ethyl Acetoacetate

Abstract

A new spectrofluorimetric method has been developed and validated for the quantification of ceftriaxone in bulk powder, pharmaceutical formulations and spiked human plasma. The developed method is reproducible, accurate, sensitive and cost effective. In this method, ceftriaxone was converted into a fluorescent compound by reacting with 0.8 M ethyl acetoacetate and 25% formaldehyde in a buffered medium (pH = 4.2) at 90 °C. The excitation and emission wavelengths of the fluorescent reaction product are 316 nm and 388 nm respectively. Optimization of the experimental conditions affecting the condensation reaction were carefully carried out and the optimum experimental conditions were incorporated in the procedure. The developed method has a broad linear range (0.2-20 μg mL(-1)) with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 1.94 × 10(-2) μg mL(-1) and 6.47 × 10(-2) μg mL(-1) respectively. The common excipients and co-administered drugs were investigated for their interferences effect in the assay. The developed method was validated statistically through recovery studies and successfully applied to ceftriaxone determination in bulk powder, pharmaceutical formulations and spiked human plasma samples. The percent recoveries were found to be in the range of 99.04-100.26% for bulk powder, 98.88-99.92% for pharmaceutical formulations and 94.22-98.48% for spiked human plasma. The results were verified by comparing with reference literature HPLC method and were found in good agreement.

Novel Hyperbranched Polysiloxanes Containing Acetoacetyl Groups Synthesized Through Transesterification Reaction

“…The acetoacetyl group containing a reactive methylene between two carbonyl moieties is an attractive candidate, which can react with formaldehyde irreversibly at room temperature. [ 25 ] Here, a color indicator consisting of AHMT was used to visually display the formaldehyde removal effect, and the detailed procedure can be found in Supporting Information. The reaction for presence of HCHO was executed at ambient temperature and the color change effect was shown in Figure S7 (Supporting Information).…”

A Review of Methods for Removal of Ceftriaxone from Wastewater

“…The electrochemical analysis of active pharmaceuticals is based on redox processes that occur via electron transfer channels [ 66 ]. Electrochemical methods have been used for the analysis of antibiotics such as clarithromycin and azithromycin [ 67 ], diclofenac [ 68 ], and cefixime [ 69 ].…”

Development and Validation of HPLC Method for Simultaneous Determination of Ceftriaxone and Cefaclor in Commercial Formulations and Biological Samples

“…In the literature a number of analytical methods have been reported for the determination of ceftriaxone and cefaclor in pharmaceutical formulation and biological samples. These include spectrophotometric [6][7][8][9][10], spectrofluorimetric [11][12][13], and chromatographic [14][15][16][17][18][19][20][21][22][23][24]. In the recent past the quality of drugs especially antibacterial agents has been compromised worldwide particularly in the developing countries.…”