Spectrofluorimetric Determination of Fluvoxamine in Dosage Forms and Plasma Via Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole

Abstract

A highly sensitive and simple spectrofluorimetric method has been developed and validated for the determination of the antidepressant fluvoxamine (FXM) in its dosage forms and plasma. The method was based on nucleophilic substitution reaction of FXM with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in an alkaline medium (pH 8) to form a highly fluorescent derivative that was measured at 535 nm after excitation at 470 nm. The factors affecting the reaction was carefully studied and optimized. The kinetics of the reaction was investigated, and the reaction mechanism was presented. Under the optimized conditions, linear relationship with good correlation coefficient (0.9995) was found between the fluorescence intensity and FXM concentration in the range of 65-800 ng ml(-1). The limits of detection and quantitation for the method were 21 and 64 ng ml(-1), respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 2.17%. The proposed method was successfully applied to the determination of FXM in its pharmaceutical tablets with good accuracy; the recovery values were 97.8-101.4 +/- 1.08-2.75%. The results obtained by the proposed method were comparable with those obtained by the official method. The high sensitivity of the method allowed its successful application to the analysis of FXM in spiked human plasma. The proposed method is superior to the previously reported spectrofluorimetric method for determination of FXM in terms of its simplicity. The proposed method is practical and valuable for its routine application in quality control and clinical laboratories for analysis of FXM.

Potassium permanganate–acridine yellow chemiluminescence system for the determination of fluvoxamine, isoniazid and ceftriaxone

“…1a), (E)-5-methoxy-1-[4-(trifluoromethyl)-phenyl]-1pentanone-O-2-aminoethyl) oxime maleate, is a well-tolerated antidepressant that functions as a selective serotonin reuptake inhibitor and is used in the treatment of depression and obsessivecompulsive disorders (1). Various methods have been developed for the quantification of FLU, and these are usually based on chromatographic analysis (1)(2)(3)(4)(5)(6)(7). CEF (Fig.…”

Sensitive and selective determination of fluvoxamine maleate using a sensitive chemiluminescence system based on the alkaline permanganate–Rhodamine B–gold nanoparticles reaction

“…A number of analytical methods including high-performance liquid chromatography (12)(13)(14)(15), electrochemical techniques (16), capillary electrophoresis (17), spectrophotometry (18)(19)(20)(21) and fluorometry (22,23) have been proposed for the determination of Flu in biological fluids. However, quality control for such drugs demands simple, rapid and low-cost analytical methods.…”

Validated high-performance thin-layer chromatographic method for quantitation of fluvoxamine in the presence of degradation products formed under ICH recommended stress conditions

“…Fluvoxamine was determined by gas chromatography [7]. Several methods like high-performance liquid chromatography (HPLC) [8][9][10][11][12][13][14][15][16][17][18][19][20], spectrofluorimetry [21], and fluorine magnetic resonance spectroscopy [22] have been developed for determination of fluvoxamine in plasma. Determination of fluvoxamine in pharmaceutical preparation by HPLC [23] and capillary electrophoresis [24] method have been reported in literature.…”