Spectrofluorimetric and image recordings of spontaneous and lectin-induced cytosolic calcium oscillations in Jurkat T cells

Payet, M; Bilodeau, Lyne; Héroux, Jennifer; Harbec, G.; Dupuis, Gilles

doi:10.1016/0143-4160(91)90048-j

Cited by 20 publications

(8 citation statements)

References 44 publications

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“…These investigators found that some Jurkat cells (variant E6.1) attached to coverslips coated with L-polylysine or stimulated by adding it to the bathing solution, presented a relatively stable pattern of oscillations over periods of time up to 3 h. The same patterns of Ca 2ϩ oscillations in this cell line have also been reported elsewhere [Payet et al, 1991]. Such cells were then used as their own controls by repeatedly turning on and off the external electromagnetic field while continuously registering intracellular Ca 2ϩ level.…”

Section: Introductionsupporting

confidence: 55%

“…First, as mentioned in the Introduction section, the variability in oscillation patterns exhibited by Jurkat cells [Payet et al, 1991;Galvanovskis et al; did not permit a straightforward comparison of oscillation characteristics between exposed and nonexposed control cells. The use of repeated exposure intervals interposed with nonexposure intervals as controls on a single cell was found to reduce the scatter in the results.…”

Section: Exposure Protocolmentioning

confidence: 98%

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Cytoplasmic Ca2+ oscillations in human leukemia T-cells are reduced by 50 Hz magnetic fields

Galvanovskis

Sandblom

Bergqvist

et al. 1999

Bioelectromagnetics

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Section: Introductionsupporting

confidence: 55%

Section: Exposure Protocolmentioning

confidence: 98%

Cytoplasmic Ca2+ oscillations in human leukemia T-cells are reduced by 50 Hz magnetic fields

Galvanovskis

Sandblom

Bergqvist

et al. 1999

Bioelectromagnetics

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“…Results clearly indicated that CnIV-coated microspheres were effective in inducing Ca 2ϩ responses in individual cells. We [61] and others [62] have previously reported that individual Jurkat T cells display characteristic patterns of Ca 2ϩ responses. Results shown here (Fig.…”

Section: Discussionmentioning

confidence: 97%

Differential recruitment of α2β1 and α4β1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin

Holleran¹,

Barbar²,

Payet

et al. 2003

Journal of Leukocyte Biology

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Collagen type IV (CnIV) and fibronectin (Fn) were used as ligands to study the distribution of alpha(2)beta(1) and alpha(4)beta(1) integrins in low-density, detergent-resistant microdomains (DRM) of Jurkat lymphocytes. CnIV-coated microspheres induced (optical trapping) the redistribution of GM(1)-associated fluorescence from the cell periphery to the area of contact. This was not observed in cells treated with beta-methyl cyclodextrin (MCD). Fn- or bovine serum albumin-coated microspheres did not modify the peripheral distribution of fluorescence. These observations were confirmed by confocal microscopy. Western blot analysis of cells exposed to surfaces coated with CnIV revealed that the alpha(2)-subunit was initially present at low levels in DRM, became strongly associated after 40 min, and returned to basal levels after 75 min. Fn induced a slight recruitment of the beta(1)-integrin alpha(4)-subunit in DRM after 5 and 10 min, followed by a return to basal levels. Neither CnIV nor Fn triggered significant changes in the distribution of the beta(1)-subunit in DRM. Fn- and CnIV-coated microspheres or surfaces coated with these ligands triggered a MCD-sensitive mobilization of Ca(2)(+). MCD did not alter the state of the Ca(2)(+) reserves. The differential distributions of the alpha(2)beta(1) and alpha(4)beta(1) integrins in DRM may provide one additional step in the regulation of outside-in signaling involving these integrins.

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“…ECV 304 cells or HUVEC were grown on circular (25 mm) coverslips whereas Jurkat and peripheral T cells were attached to poly-L-lysine-coated coverslips [49]. The cells were washed twice with PBS and the coverslips were placed in 1 ml of incubation solution (pH 7.4) containing 115 mM NaCl, 5 mM KCl, 10 mM NaHCO 3 , 25 mM Hepes, 0.5 mM MgCl 2 , 1 mM CaCl 2 , 5.6 mM glucose and 1 mg/ml BSA and left at 4°C for 30 min.…”

Section: Time-course Analysis Of Immunofluorescence Distribution In Lmentioning

confidence: 99%

“…ECV 304 cells or HUVEC were grown on circular coverslips whereas Jurkat cells and peripheral T cells were bound to poly-L-lysine-coated coverslips [49]. The cells were exposed to the anti-VCAM-1 or to the anti- § 4 -subunit respective primary mAb as described above (4°C) and washed twice with cold PBS.…”

Section: Quantification Of Time-dependent Immunofluorescence Distribumentioning

confidence: 99%

VCAM-1 is internalized by a clathrin-related pathway in human endothelial cells but its α4β1 integrin counter-receptor remains associated with the plasma membrane in human T lymphocytes

1998

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Lymphocyte extravasation involves a step(s) of de-adhesion to allow trans- and subendothelial migration in response to inflammatory signals. We show here that ligated VCAM-1 was rapidly internalized (t1/2 14.5 min) in ECV 304 endothelial cells and in TNF-alpha-primed human umbilical vein-derived endothelial cells (t1/2 11.2 min). The process required energy (ATP), intracellular Ca2+, an intact cytoskeletal network and active protein kinases. The internalization of VCAM-1 involved a clathrin-dependent pathway based on the observations that 1) it was inhibited in cells treated with lysosomotropic agents or with a hypertonic concentration of sucrose, and 2) internalized VCAM-1 colocalized with clathrin. In contrast, the cross-linked alpha 4 beta 1 integrin counter-receptor of VCAM-1 remained associated with the plasma membrane of purified peripheral T and Jurkat cells. Our results suggest a model where VCAM-1 would initially participate in the retention of T cells to the endothelium by binding alpha 4 beta 1 integrin. Lymphocyte de-adhesion would be facilitated as a result of the internalization of VCAM-1. The persistent cell surface expression of alpha 4 beta 1 integrin would allow the migrating T cells to interact with and receive signal(s) from its fibronectin ligand of the extracellular matrix.

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Spectrofluorimetric and image recordings of spontaneous and lectin-induced cytosolic calcium oscillations in Jurkat T cells

Cited by 20 publications

References 44 publications

Cytoplasmic Ca2+ oscillations in human leukemia T-cells are reduced by 50 Hz magnetic fields

Cytoplasmic Ca2+ oscillations in human leukemia T-cells are reduced by 50 Hz magnetic fields

Differential recruitment of α2β1 and α4β1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin

VCAM-1 is internalized by a clathrin-related pathway in human endothelial cells but its α4β1 integrin counter-receptor remains associated with the plasma membrane in human T lymphocytes

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