Spectro-electrochemical studies of acetylcholinesterase in carbon nanofiber-bioinspired silica nanocomposites for biosensor development

Hatzimarinaki, Maria; Vamvakaki, Vicky; Chaniotakis, Nikos A.

doi:10.1039/b811350e

Cited by 20 publications

(15 citation statements)

References 43 publications

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“…[44] Forster et al [44] reported a novel protein array featuring a patterned assembly of vertically aligned single-walled carbon nanotube (SWCNT) forests formed using inkjet printing on transparent ITO (indium tin oxide) substrates via a much simpler approach, as illustrated in Figure 3. Although CNTs can effectively overcome the poor conductivity caused by porous silica nanoparticles, [35] there are some drawbacks to impede the further development and application of CNTs including uncontrollable diameters, broad distribution and flexible structure. Therefore, combining silica nanoparticles with CNTs through the formation of a SiO 2 shell on the surface of CNTs will not only effectively increase the solubility of CNTs, but also exhibit a high surface area with a porous structure, resulting in a satisfactory biomaterial for constructing high-sensitivity electrochemical aptasensors.…”

Section: Carbon Nanotubeàsilica Composite Matrixmentioning

confidence: 99%

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Recent advances in silica-based biosensors: a review

Chen

Zhang

Chang

et al. 2015

Journal of the Chinese Advanced Materials Society

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Silica has become a common platform for various catalytic and chemical reactions, biosensor, and drug release owing to its low toxicity, chemical and physical stabilities, large surface area, tunable pore sizes, and well-defined surface chemistry. Therefore, biomolecules encapsulated within a porous silica host has provoked significant interest in the development, application, and market of silica-based biosensors due to its potential application in analytic chemistry and biological science. Considerable progresses have been achieved in past few decades. This review focuses primarily on such advances in synthesis and applications of silica-based biosensors, especially emphasizing development which has occurred in the last 10 years, further driving readers to understand and construct various silica-based biosensors with high sensitivity and high stability.

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Section: Carbon Nanotubeàsilica Composite Matrixmentioning

confidence: 99%

“…Evidently, this is a very serious challenge because many applications involve enzymes catalytic process. [35] As a result, the considerable endeavours should be undertaken to construct efficient electron transfer between the active centre of the biomolecules and conductive transducers.…”

Section: Porous Silica Supportmentioning

confidence: 99%

Recent advances in silica-based biosensors: a review

Chen

Zhang

Chang

et al. 2015

Journal of the Chinese Advanced Materials Society

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“…In two very recent papers [34][35], CNFs have been used for the immobilization of acetylcholinesterase and then encapsulated into poly(L-lysine) templated silica nanocomposites. The NFs/silica architecture proved to highly improve the stability of the enzyme inside the encapsulation matrix.…”

Section: Carbon Nanofibers In Biosensorsmentioning

confidence: 99%

The applications of carbon nanostructures and semiconductor materials in the development of biosensors

Frasco

Buiculescu

Vamvakaki

et al. 2009

2009 International Semiconductor Conference

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In recent years, nanomaterials and nanostructures with unique chemical, physical, and mechanical properties have been developed and applied as both sensing matrices and transducers, offering new opportunities for the development of highly sensitive bio-chemical sensors. The chemical and physical characteristics of the electrochemically active carbon nanotubes, nanofibers and fullerenes, as well as their potential applications in biosensors will be first analyzed. In addition, the advances that have been made in the area of biosensors based on semiconductor materials and quantum dots will be presented. The advantages of the use of these two families of nanomaterials in the design of new devices are thus very promising towards future envisioned biosensor applications.

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“…Even though there are not many reports on biosensors making use of the biosilica, due to the novelty of this process, the majority of them are based on electrochemistry. The entrapment of glucose oxidase [63] and acetylcholinesterase [60,64] into biosilica, in the presence of carbon nanotubes, has been shown to allow the direct electron transfer from the enzyme to the immobilization surface. Biocatalytic precipitation of silica composites was realized with lysozyme providing the scaffold for silica formation in the case of glucose oxidase (GOx), and with poly(L-lysine) polymer in the case of acetylcholinesterase (AChE), both methods using tetramethyl orthosilicate (TMOS) as a silicic acid precursor.…”

Section: Biosensor Developmentmentioning

confidence: 99%

Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

Chaniotakis

Buiculescu

2012

Biomedical Materials and Diagnostic Devices

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Biosilicification is a process by which silica-based nanostructures are formed (biosilica) through a biopolymer-based catalysis. Found in nature, biosilica is produced by many organisms and is renowned for its elaborate shapes down to nanoscale size. Biosilica is physically very strong, biocompatible, and has unique optical and chemical properties. Its surface is covered with chemically functional groups which are ideal for functionalization, protein adsorption and enzyme stabilization. Biomolecules entrapped within biosilica are highly stabilized from chemical denaturation, while protected from protease attacks. The ability to synthesize biosilica in the laboratory has set the grounds for the beginning of a new technological era which will provide us with novel devices and tools applicable to a variety of technologies. This chapter is devoted to the discussion of the engineering applications of biosilica in the biomedical and sensing areas.

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Spectro-electrochemical studies of acetylcholinesterase in carbon nanofiber-bioinspired silica nanocomposites for biosensor development

Cited by 20 publications

References 43 publications

Recent advances in silica-based biosensors: a review

Recent advances in silica-based biosensors: a review

The applications of carbon nanostructures and semiconductor materials in the development of biosensors

Biosilica – Nanocomposites – Nanobiomaterials for Biomedical Engineering and Sensing Applications

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