The use of highly activated carbon nanofibers for the design of catalytic electrochemical biosensors is demonstrated. The direct immobilization of enzymes onto the surface of carbon nanofibers is shown to be a highly efficient method for the development of a new class of very sensitive, stable, and reproducible electrochemical biosensors. These results establish the fact that the carbon nanofiber is the best matrix so far described for the development of biosensors, far superior to carbon nanotubes or graphite powder.

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