Spectro-Electrochemical Investigation of the bc1 Complex from the Yeast Saccharomyces cerevisiae using Surface Enhanced B-Band Resonance Raman Spectroscopy

Schach, Denise; Grosserüschkamp, Marc; Nowak, Christoph; Knoll, Wolfgang; Naumann, Renate

doi:10.5772/13872

Cited by 3 publications

(1 citation statement)

References 40 publications

(63 reference statements)

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“…The bc 1 complex from yeast Saccharomyces cerevisiae was adsorbed onto a cysteamine-functionalized gold electrode and reconstituted into a bilayer lipid membrane. 559 The immobilized protein was characterized by SERR spectroelectrochemistry and cyclic voltammetry. The voltammograms at pH 7 under anaerobic conditions showed an irreversible cathodic process.…”

Section: Bc 1 Complexmentioning

confidence: 99%

Redox Properties of the Membrane Proteins from the Respiratory Chain

Melin

Hellwig

2020

Chem. Rev.

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This review focuses on the electrochemical and spectroelectrochemical studies that gave insight into redox potentials of the four mitochondrial complexes and their homologues from bacterial respiratory chains using O2 as a terminal acceptor, thus providing crucial information about their reaction mechanism. Advantages and limitations of the use of the different techniques for the study of membrane proteins are presented. Electrocatalytic experiments are described that revealed specific features of the reaction with the substrates and inhibitors. An overview is given on the great variability of the redox and catalytic properties of the enzymes in different organisms that may be due to adaptation to the specific environments in which these enzymes function. The adaptation of the redox chain to the different types of quinone and substrates is analyzed, and future studies are discussed.

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Section: Bc 1 Complexmentioning

confidence: 99%

Redox Properties of the Membrane Proteins from the Respiratory Chain

Melin

Hellwig

2020

Chem. Rev.

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Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism

Radić

Pavlović

Lazović³

et al. 2017

Environ Sci Pollut Res

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Modern, efficient, and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO·5HO) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO·5HO showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.

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