Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism

Radić, Danka; Pavlović, Vera P.; Lazović, Milana; Jovičić-Petrović, Jelena; Karličić, Vera; Lalević, Blažo; Raičević, Vera

doi:10.1007/s11356-017-9817-4

Cited by 12 publications

(4 citation statements)

References 35 publications

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“…Vishniac, ). Some strains of the species Y. lipolytica , S. cerevisiae , Kluyveromyces thermotolerans and Sporobolomyces roseus exhibit high tolerance to Zn and Cr (Raspor & Zupan, ; Krauter & Krauter, ; Malik, ; Radiæ et al, ).…”

Section: Major Factors In Extreme Habitats: Hard But Not Impossiblementioning

confidence: 99%

Extremophilic yeasts: the toughest yeasts around?

Buzzini

Turchetti

Yurkov

2018

Yeast

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Microorganisms are widely distributed in a multitude of environments including ecosystems that show challenging features to most life forms. The combination of extreme physical and chemical factors contributes to the definition of extreme habitats although the definition of extreme environments changes depending on one's point of view: anthropocentric, microbial-centric or zymo-centric. Microorganisms that live under conditions that cause hard survival are called extremophiles. In particular organisms that require extreme conditions are called true extremophiles while organisms that tolerate them to some extent are termed extremotolerant. Deviation of temperature, pH, osmotic stress, pressure and radiation from the common range delineates extreme environments. Yeasts are versatile eukaryotic organisms that are not frequently considered the toughest microorganisms in comparison with prokaryotes. Nevertheless extremophilic or extremotolerant species are present also within this group. Here a brief description is provided of the main extreme habitats and the metabolic and physiological modifications adopted by yeasts depending on their adverse conditions. Additionally the main extremophilic and extremotolerant yeast species associated with a few extreme habitats are listed.

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Section: Major Factors In Extreme Habitats: Hard But Not Impossiblementioning

confidence: 99%

Extremophilic yeasts: the toughest yeasts around?

Buzzini

Turchetti

Yurkov

2018

Yeast

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“…Copper can be removed in two steps: cell surface bioaccumulation of cell walls and intracellular bioaccumulation of heavy metal transporters ( Duncan and Brady, 1994 ; Li et al, 2014 ). Yeasts exhibit good copper bioaccumulation ability ( Radic et al, 2017 ). Microbes have evolved different strategies to resist the toxicity of heavy metals.…”

Section: Introductionmentioning

confidence: 99%

Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

Yan

Sun

et al. 2021

Front. Microbiol.

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Various agricultural products used in food fermentation are polluted by heavy metals, especially copper, which seriously endangers human health. Methods to remove copper with microbial strategies have gained interests. A novel Meyerozyma guilliermondii GXDK6 could survive independently under high stress of copper (1400 ppm). The copper tolerance mechanism of GXDK6 was revealed by integrated omics in this work. Whole-genome analysis showed that nine genes (i.e., CCC2, CTR3, FRE2, GGT, GST, CAT, SOD2, PXMP4, and HSP82) were related to GXDK6 copper tolerance. Copper stress elevated glutathione metabolism-related gene expression, glutathione content, and glutathione sulfur transferase activity, suggesting enhanced copper conjugation and detoxification in cells. The inhibited copper uptake by Ctr3 and enhanced copper efflux by Ccc2 contributed to the decrease in intracellular copper concentration. The improved expression of antioxidant enzyme genes (PXMP4, SOD2, and CAT), accompanied by the enhanced activities of antioxidant enzymes (peroxidase, superoxide dismutase, and catalase), decreased copper-induced reactive oxygen species production, protein carbonylation, lipid peroxidation, and cell death. The metabolite D-mannose against harsh stress conditions was beneficial to improving copper tolerance. This study contributed to understanding the copper tolerance mechanism of M. guilliermondii and its application in removing copper during fermentation.

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“…Studies on the biological adsorption of heavy metals by yeast are available and mainly focus on the factors influencing biosorption (Brady & Duncan, 1994; Vasudevan et al ., 2002), as well as the dynamic models (Vasudevan et al ., 2003) and mechanisms of biosorption (Avery et al ., 1996; Suh et al ., 1998; Sun et al ., 2016; Liu et al ., 2017; Radic et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%