Spectral spatial electron paramagnetic resonance imaging as a tool to study photoactive dimethacrylate-based dental resins

Lévêque, Philippe; Leprince, Julian; Bebelman, Sabine; Devaux, Jacques; Leloup, Gaëtane; Gallez, Bernard

doi:10.1016/j.jmr.2012.04.014

Cited by 12 publications

(5 citation statements)

References 43 publications

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“…It means that if the spin probe administration is disturbed, it will be visible in the spatial image. Therefore, for precise localization of the tissue boundaries, EPRI should be supported by other anatomical imaging techniques such as MRI, 19 CT, 52 or US. 53 Although the anatomical resolution of EPRI is far from the techniques mentioned above, the development of a new spin probe with a narrow EPR linewidth will improve the spatial resolution and provide more details of the spin probe biodistribution.…”

Section: ■ Discussionmentioning

confidence: 99%

Dynamic Electron Paramagnetic Resonance Imaging: Modern Technique for Biodistribution and Pharmacokinetic Imaging

Baranowski

Gonet

Czechowski³

et al. 2020

J. Phys. Chem. C

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The imaging of the biodistribution and pharmacokinetics is critical in understanding the complexity of drug delivery mechanisms, the status of the disease, and the monitoring of the treatment progress. The imaging techniques, such as magnetic resonance imaging, computed tomography, and positron emission tomography, are suitable for clinical applications. However, with regards to the biodistribution and pharmacokinetics, their availability is often limited due to the requirements of ionizing radiation or high magnetic fields, low spatial and temporal resolution (applicable for animals), and high maintenance costs. Electron paramagnetic resonance (EPR) imaging is a technique that allows for the minimal invasive mapping of unique parameters, such as the oxygen concentration, the redox state, the thiol concentration, or the pH level, in vivo. In this work, a high temporal resolution 3D EPR imaging technique was used for assessing the trityl spin probe pharmacokinetics in mice. The results demonstrate the preliminary outcomes in the application of EPR imaging for the comparison of the trityl spin probe pharmacokinetics between that of a healthy mouse and a tumor-bearing mouse. This study will stimulate further investigation into the use of imaging strategies, such as EPR imaging, to analyze pharmacokinetics.

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Section: ■ Discussionmentioning

confidence: 99%

Dynamic Electron Paramagnetic Resonance Imaging: Modern Technique for Biodistribution and Pharmacokinetic Imaging

Baranowski

Gonet

Czechowski³

et al. 2020

J. Phys. Chem. C

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“…In addition, other characteristics of the material may have contributed to these results, such as the organic matrix composition, as the polymerization level varies according to the amount of the monomer present in the composite resins (20) . The increase in time of cure of Flash max p3 and Wood pecker from 10 seconds to 20 seconds gives higher microhardness values and greater depth of cure for all types of bulkfill composites while the high intensity with short curing time (Flash max p3 for 3 seconds) revealed lowest microhardness value in comparison to other groups of light curing protocol.…”

Section: Discussionmentioning

confidence: 99%

The Effect of LED Light on Depth of Cure and Microhardness of Three Types of Bulkfill Composite

Mohannad¹,

Baban²

2016

JBCD

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Background: To evaluate the ISO depth of cure of bulkfill composites and depth of cure which determined by Vickers microhardness test. Materials and Methods: Bulkfill resin composite specimens (n=150) were prepared of three bulkfill composite materials (TetricEvo Ceram, Quixfil and SDR) and light cured by Flash max p3 for 3, 10, 20 seconds and by wood pecker for 10, 20 seconds respectively, a mold was filled with one of the three bulkfill composites and light cured. The specimens removed from the mold and scraped by plastic spatula and the remaining length (absolute length) was measured which represent the ISO depth of cure. After that the specimens were returned into the mold and a microhardness indentation device applied on the specimen and hardness measurements (Vickers hardness, VHN) were made at defined distance, beginning at the resin composite that had been closest to the light curing unit (i.e. at the top) and proceeding toward the uncured resin composite (i.e. toward the bottom) on the basis of the VHN measurement, Vickers hardness test generated for each group. Results and Conclusion: ISO depth of cure of bulkfill composite materials is time and type of light curing protocol dependent rather than type of material of bulkfill composite while the depth of cure determined by Vickers hardness number is material dependent in addition to the light curing protocol.

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“…However, the most commonly used nitroxyl spin probes have a much larger linewidth (Δ H ∼ 1 G). Furthermore, since finite limits exist on the maximum G x that can be applied, Δ x cannot be reduced for unlabeled nitroxyls by simply increasing G x . , Even, the use of Fourier transform (FT)-based methods has not been helpful for the unlabeled nitroxides due to their short spin–spin relaxation time, T 2e . Hence, isotopically labeled ( 15 N, 2 D)-nitroxyls are preferred, , which makes the whole experiment quite expensive and synthetically demanding.…”

Section: Introductionmentioning

confidence: 99%

Harnessing Electron Spin Hyperpolarization in Chromophore–Radical Spin Probes for Subcellular Resolution in Electron Paramagnetic Resonance Imaging: Concept and Feasibility

Rane

2022

J. Phys. Chem. B

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Obtaining a subcellular resolution for biological samples doped with stable radicals at room temperature (RT) is a long-sought goal in electron paramagnetic resonance imaging (EPRI). The spatial resolution in current EPRI methods is constrained either because of low electron spin polarization at RT or the experimental limitations associated with the field gradients and the radical linewidth. Inspired by the recent demonstration of a large electron spin hyperpolarization in chromophore-nitroxyl spin probe molecules, the present work proposes a novel optically hyperpolarized EPR imaging (OH-EPRI) method, which combines the optical method of two-photon confocal microscopy for hyperpolarization generation and the rapid scan (RS) EPR method for signal detection. An important aspect of OH-EPRI is that it is not limited by the abovementioned restrictions of conventional EPRI since the large hyperpolarization in the spin probes overcomes the poor thermal spin polarization at RT, and the use of two-photon optical excitation of the chromophore naturally generates the required spatial resolution, without the need for any magnetic field gradient. Simulations based on time-dependent Bloch equations, which took into account both the RS field modulation and the hyperpolarization generation by optical means, were performed to examine the feasibility of OH-EPRI. The simulation results revealed that a spatial resolution of up to 2 fL can be achieved in OH-EPRI at RT under in vitro conditions. Notably, the majority of the requirements for an OH-EPRI experiment can be fulfilled by the currently available technologies, thereby paving the way for its easy implementation. Thus, the proposed method could potentially bridge the sensitivity gap between the optical and magnetic imaging techniques.

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Spectral spatial electron paramagnetic resonance imaging as a tool to study photoactive dimethacrylate-based dental resins

Cited by 12 publications

References 43 publications

Dynamic Electron Paramagnetic Resonance Imaging: Modern Technique for Biodistribution and Pharmacokinetic Imaging

Dynamic Electron Paramagnetic Resonance Imaging: Modern Technique for Biodistribution and Pharmacokinetic Imaging

The Effect of LED Light on Depth of Cure and Microhardness of Three Types of Bulkfill Composite

Harnessing Electron Spin Hyperpolarization in Chromophore–Radical Spin Probes for Subcellular Resolution in Electron Paramagnetic Resonance Imaging: Concept and Feasibility

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