Spectral Fluorescence Measurements

Zwinkels, Joanne C.; DeRose, Paul C.; Leland, James E.

doi:10.1016/b978-0-12-386022-4.00007-8

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…Fluorescence intensity was measured using an Ocean Optics USB4000+ spectrometer 2000 (Ocean Optics, Orlando, FL, USA) in combination with a 30 mm wide integrating sphere under (8:di) measuring geometry (diffuse geometry, specular component included) with the addition of an LSM Series LED 2000 (Ocean insight, Orlando, FL, USA) light source at 365 nm as the fluorescence excitation wavelength. The use of an integrating sphere in fluorescence measurements were previously discussed and applied in different areas [ 30 , 31 , 32 , 33 ]. The excitation light source was kept stable at a constant current of 0.140 A. Fluorescence intensity was measured in the spectral range between 330 and 630 nm.…”

Section: Methodsmentioning

confidence: 99%

Improvement in Thermochromic Offset Print UV Stability by Applying PCL Nanocomposite Coatings

et al. 2022

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Thermochromic (TC) printing inks change their colouration as a response to a change in temperature. This ability renders them attractive for various applications such as smart packaging, security printing, and marketing, but their application is limited due to their low UV stability, i.e., loss of their thermochromic effect when exposed to UV radiation. In order to improve the UV stability of TC prints, one offset TC printing ink was printed and coated with nanomodified polycaprolactone (PCL) coating. The coating was prepared with the incorporation of 1%, 2%, and 3% mass ratios of ZnO and TiO2 nanoparticles in the PCL matrix. The prepared nanocomposite coatings were applied onto the TC print and exposed to UV radiation; afterwards, they were characterized by the colour properties of prints, SEM microscopy, FTIR, and fluorescence spectroscopy. SEM microscopy, FTIR, and fluorescence spectroscopy showed higher rates of polymer degradation, and the results of colour stability indicated that 3% TiO2 in PCL matrix gave the best UV stability and protection of TC prints.

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Section: Methodsmentioning

confidence: 99%

Improvement in Thermochromic Offset Print UV Stability by Applying PCL Nanocomposite Coatings

et al. 2022

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“…The excitation intensity near 300 nm was decreased for both dyes in the presence of glucose because the contribution of the plastic well plate to the excitation decreased. In addition, we compared the quantum yield of the two analogs by measuring the relative quantum yield [32]. The relative quantum yield is calculated using a flowing equation: (2…”

Section: Glucose-dependent Optical Characterisitcsmentioning

confidence: 99%

Acetylated Trifluoromethyl Diboronic Acid Anthracene with a Large Stokes Shift and Long Excitation Wavelength as a Glucose-Selective Probe

et al. 2022

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Continuous control of blood glucose levels is important for the effective treatment of diabetes. The short-term use of enzymatic continuous monitoring systems involves expensive maintenance and is inconvenient, which limits their widespread use by diabetes patients. The fluorescent diboronic anthracene-embedded system has demonstrated in vivo continuous glucose monitoring for 12 times longer than enzymatic systems by protecting the dye from reactive oxygen species. However, its small Stokes shift and low excitation and emission wavelength should be heavily considered for easy fabrication. We successfully synthesized a derivative of bis-phenyl boronate with a large Stokes shift and long excitation wavelength by adding an acetyl moiety to the anthracene ring. This resulted in a ~90-nm Stokes shift and 15-nm and 80-nm redshifts of the excitation and emission wavelengths, respectively. The fluorescence of the synthesized probe increased proportionally with the glucose concentration because the formation of the boronic acid-glucose complex prevented photoinduced electron transfer. The association constant and quantum yield for acetyl-substituted diboronic anthracene with glucose was 20% and 13% higher than that of the analog, respectively. While keeping resistance to the oxidation by reactive oxygen species, the improved optical properties and glucose-detecting performances of the newly synthesized dye will allow better pairing of the source and detecting unit for in vivo continuous glucose monitoring, leading to easy fabrication and then contributing more to utilization by diabetes patients.

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“…The main uncertainty components for fluorescence measurements using a twomonochromator method and physical transfer standards are described in detail in several references [3,4,39,40]. The uncertainty budget for the new NRC Reference…”

Section: Uncertainty Budget For Sphere Geometrymentioning

confidence: 99%

Characterization of a versatile reference instrument for traceable fluorescence measurements using different illumination and viewing geometries specified in practical colorimetry—part 2: sphere geometry (8:d)

et al. 2017

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.nrc-cnrc.gc.ca/eng/view/object/?id=d9682c21-38af-4e8e-8896-f53b13c69d2a http://nparc.nrc-cnrc.gc.ca/fra/voir/objet/?id=d9682c21-38af-4e8e-8896-f53b13c69d2a AbstractIn the second part of this two-part series on the development of a versatile reference instrument at the National Research Council of Canada (NRC), we have extended the characterization of t he NRC Reference Goniospectrofluorimeter to high-accuracy fluorescence measurements in a sphere geometry (8:d) that is specified in standard test methods for many practical applications in colorimetry. This builds upon the work reported in part-one of this series which described in detail the design, characterization and validation of this new instrument for realizing a total spectral radiance factor scale in a bidirectional (45a:0) geometry. To extend the measurement capabilities to a sphere geometry, it was configured with a large diameter integrating sphere accessory.Preliminary results using a substitution-mode operating procedure showed large sphere errors that were characterized and corrected for. To improve this traceability, the sphere was modified to operate in comparison-mode and this effectively eliminated many of the sphere-related errors t hat typically limit the accuracy of sphere-based fluorescence 2 measurements. The performance of the instrument configured for a sphere geometry

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Spectral Fluorescence Measurements

Cited by 17 publications

References 33 publications

Improvement in Thermochromic Offset Print UV Stability by Applying PCL Nanocomposite Coatings

Improvement in Thermochromic Offset Print UV Stability by Applying PCL Nanocomposite Coatings

Acetylated Trifluoromethyl Diboronic Acid Anthracene with a Large Stokes Shift and Long Excitation Wavelength as a Glucose-Selective Probe

Characterization of a versatile reference instrument for traceable fluorescence measurements using different illumination and viewing geometries specified in practical colorimetry—part 2: sphere geometry (8:d)

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