Abstract:The work is targeted to confirm participation of microscopic fungi in transformation of humus substances in aquatic environments. The research is focused on the spectroscopic study of the collection of fungal strains with different pigmentation of mycelium. Spectral properties of fungal metabolites were measured and compared to that of natural aquatic nonliving organic matter and commercial humus substances in aqueous solutions. The experiments revealed that the effect of microscopic fungi growing in the cultu… Show more
“…Therefore, the absorption spectra of macromolecules' set with various compositions are structure-free. The particularity manifested as local maxima in the UV spectrum only in certain types of absorption spectra of aquatic humic substances (such as from the peat or lignosulphate) according to these investigations [1,6]. Humic substances determine the spectral properties of the DOS (dissolved organic substance) of natural water.…”
Section: Issn 2224-5278mentioning
confidence: 88%
“…The quantitative and qualitative compositions of organic contaminants presented in the aquatic ecosystems with sufficient accuracy are established by spectral methods [1] and method of georadiolocation in complex scientific applied researches [2].…”
mentioning
confidence: 99%
“…The research objects were Almaty city soils, also their samples and extracts. The soil samples have been taken at 5 patches: four in the city (P. [1][2][3][4] and one from outside the city (P. 5) by the "envelope" method at a depth of 0-25 cm [3] for determination of geological and morphological and fluorescent characteristics.…”
mentioning
confidence: 99%
“…Calculation of the fluorescence quantum efficiency F fl was carried out by the method of the reference compound, applied for samples of natural water and commercial humic preparations earlier [1, 6, etc.]. An aqueous solution of quinine sulfate was used as a reference compound, because according to the shape of the spectral line and the position of the fluorescence maximum it's close to natural humic compounds [8,1].…”
NAS RK is pleased to announce that News of NAS RK. Series of geology and technical sciences scientific journal has been accepted for indexing in the Emerging Sources Citation Index, a new edition of Web of Science. Content in this index is under consideration by Clarivate Analytics to be accepted in the Science Citation Index Expanded, the Social Sciences Citation Index, and the Arts & Humanities Citation Index. The quality and depth of content Web of Science offers to researchers, authors, publishers, and institutions sets it apart from other research databases. The inclusion of News of NAS RK. Series of geology and technical sciences in the Emerging Sources Citation Index demonstrates our dedication to providing the most relevant and influential content of geology and engineering sciences to our community. Қазақстан Республикасы Ұлттық ғылым академиясы "ҚР ҰҒА Хабарлары. Геология жəне техникалық ғылымдар сериясы" ғылыми журналының Web of Science-тің жаңаланған нұсқасы Emerging Sources Citation Index-те индекстелуге қабылданғанын хабарлайды. Бұл индекстелу барысында Clarivate Analytics компаниясы журналды одан əрі the Science Citation Index Expanded, the Social Sciences Citation Index жəне the Arts & Humanities Citation Index-ке қабылдау мəселесін қарастыруда. Webof Science зерттеушілер, авторлар, баспашылар мен мекемелерге контент тереңдігі мен сапасын ұсынады. ҚР ҰҒА Хабарлары. Геология жəне техникалық ғылымдар сериясы Emerging Sources Citation Index-ке енуі біздің қоғамдастық үшін ең өзекті жəне беделді геология жəне техникалық ғылымдар бойынша контентке адалдығымызды білдіреді. НАН РК сообщает, что научный журнал «Известия НАН РК. Серия геологии и технических наук» был принят для индексирования в Emerging Sources Citation Index, обновленной версии Web of Science. Содержание в этом индексировании находится в стадии рассмотрения компанией Clarivate Analytics для дальнейшего принятия журнала в the Science Citation Index Expanded, the Social Sciences Citation Index и the Arts & Humanities Citation Index. Web of Science предлагает качество и глубину контента для исследователей, авторов, издателей и учреждений. Включение Известия НАН РК. Серия геологии и технических наук в Emerging Sources Citation Index демонстрирует нашу приверженность к наиболее актуальному и влиятельному контенту по геологии и техническим наукам для нашего сообщества.
“…Therefore, the absorption spectra of macromolecules' set with various compositions are structure-free. The particularity manifested as local maxima in the UV spectrum only in certain types of absorption spectra of aquatic humic substances (such as from the peat or lignosulphate) according to these investigations [1,6]. Humic substances determine the spectral properties of the DOS (dissolved organic substance) of natural water.…”
Section: Issn 2224-5278mentioning
confidence: 88%
“…The quantitative and qualitative compositions of organic contaminants presented in the aquatic ecosystems with sufficient accuracy are established by spectral methods [1] and method of georadiolocation in complex scientific applied researches [2].…”
mentioning
confidence: 99%
“…The research objects were Almaty city soils, also their samples and extracts. The soil samples have been taken at 5 patches: four in the city (P. [1][2][3][4] and one from outside the city (P. 5) by the "envelope" method at a depth of 0-25 cm [3] for determination of geological and morphological and fluorescent characteristics.…”
mentioning
confidence: 99%
“…Calculation of the fluorescence quantum efficiency F fl was carried out by the method of the reference compound, applied for samples of natural water and commercial humic preparations earlier [1, 6, etc.]. An aqueous solution of quinine sulfate was used as a reference compound, because according to the shape of the spectral line and the position of the fluorescence maximum it's close to natural humic compounds [8,1].…”
NAS RK is pleased to announce that News of NAS RK. Series of geology and technical sciences scientific journal has been accepted for indexing in the Emerging Sources Citation Index, a new edition of Web of Science. Content in this index is under consideration by Clarivate Analytics to be accepted in the Science Citation Index Expanded, the Social Sciences Citation Index, and the Arts & Humanities Citation Index. The quality and depth of content Web of Science offers to researchers, authors, publishers, and institutions sets it apart from other research databases. The inclusion of News of NAS RK. Series of geology and technical sciences in the Emerging Sources Citation Index demonstrates our dedication to providing the most relevant and influential content of geology and engineering sciences to our community. Қазақстан Республикасы Ұлттық ғылым академиясы "ҚР ҰҒА Хабарлары. Геология жəне техникалық ғылымдар сериясы" ғылыми журналының Web of Science-тің жаңаланған нұсқасы Emerging Sources Citation Index-те индекстелуге қабылданғанын хабарлайды. Бұл индекстелу барысында Clarivate Analytics компаниясы журналды одан əрі the Science Citation Index Expanded, the Social Sciences Citation Index жəне the Arts & Humanities Citation Index-ке қабылдау мəселесін қарастыруда. Webof Science зерттеушілер, авторлар, баспашылар мен мекемелерге контент тереңдігі мен сапасын ұсынады. ҚР ҰҒА Хабарлары. Геология жəне техникалық ғылымдар сериясы Emerging Sources Citation Index-ке енуі біздің қоғамдастық үшін ең өзекті жəне беделді геология жəне техникалық ғылымдар бойынша контентке адалдығымызды білдіреді. НАН РК сообщает, что научный журнал «Известия НАН РК. Серия геологии и технических наук» был принят для индексирования в Emerging Sources Citation Index, обновленной версии Web of Science. Содержание в этом индексировании находится в стадии рассмотрения компанией Clarivate Analytics для дальнейшего принятия журнала в the Science Citation Index Expanded, the Social Sciences Citation Index и the Arts & Humanities Citation Index. Web of Science предлагает качество и глубину контента для исследователей, авторов, издателей и учреждений. Включение Известия НАН РК. Серия геологии и технических наук в Emerging Sources Citation Index демонстрирует нашу приверженность к наиболее актуальному и влиятельному контенту по геологии и техническим наукам для нашего сообщества.
“…Multiplexed detection of antigens in immunoassays has been the most successful strategy using fluorescent probe molecules which are spatially isolated from each other on beads or different locations on a surface [1][2][3]. Detection schemes for multiple targets have been extensively studied and are commercially available [4][5][6]. The greatest successes have involved spatially isolated formats such as planar arrays, beads, and separate assays which run concurrently [7][8][9][10].…”
This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method. First, goat anti-human IgG was used to capture human IgG (model antigen); goat anti-human IgG (Cy3 or FITC) was used to detect the model antigen; a saturating level of model antigen was then added followed by unlabelled goat anti-human IgG (model antibody); finally, Cy3 labelled rabbit anti-goat IgG was used to detect the model antibody. Two approaches were applied to the concomitant assay to analyze the feasibility. The first approach applied FITC and Cy3 when both targets were present at the same time, resulting in 50 ng/mL of the antibody detection limit and 10 ng/mL of antigen detection limit in the quantitative measurements of target concentration, taking the consideration of FRET efficiency of 68% between donor and acceptor. The sequential approach tended to lower the signal/noise (S/N) ratio and the detection of the model antigen (lower than 1 ng/mL) had better sensitivity than the model antibody (lower than 50 ng/mL). This combined antigen/antibody method might be useful for combined detection of antigens and antibodies. It will be helpful to screen for both antigen and antibody particularly in the situations of the multiserotype and high-frequency mutant virus infections.
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