Spectral approach to recognize spherical particles among non-spherical ones by angle-resolved light scattering

Yastrebova, Ekaterina S.; Dolgikh, I. I.; Gilev, Konstantin V.; Vakhrusheva, Irina V.; Liz, Elizaveta; Litvinenko, Alena L.; Nekrasov, Vyacheslav M.; Strokotov, Dmitry I.; Карпенко, А. А.; Maltsev, Valeri P.

doi:10.1016/j.optlastec.2020.106700

Cited by 13 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The median errors play a role of the numerical indicator of analytical quality or reliability formed by three factors above mentioned. We assume that difference between the geometry of the real erythrocyte and its optical model gives maximal contribution into the error because the near‐perfect sphered erythrocytes can be analyzed with the SFC technology with the median errors of 1.2 fl and 0.6 pg for volume and hemoglobin content, respectively [50].…”

Section: Resultsmentioning

confidence: 99%

“…The points with error bar relate to the relative number of cells measured per time unit. The line is the fitted curve described by equations from the Reference[3] with parameters shown in the frame of the real erythrocyte and its optical model gives maximal contribution into the error because the near-perfect sphered erythrocytes can be analyzed with the SFC technology with the median errors of 1.2 fl and 0.6 pg for volume and hemoglobin content, respectively[50].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

et al. 2022

Self Cite

View full text Add to dashboard Cite

Molecular/cell level of gas exchange function assumes the accurate measurement of erythrocyte characteristics and rate constants concerning to molecules involved into the CO 2 /O 2 transport. Unfortunately, common hematology analyzers provide the measurement of eight indices of erythrocytes only and say little about erythrocyte morphology and nothing about rate constants of cellular function. The aim of this study is to demonstrate the ability of the Scanning Flow Cytometer (SFC) in the complete morphological analysis of mature erythrocytes and characterization of erythrocyte function via measurement of lysing kinetics. With this study we are introducing 48 erythrocyte indices. To provide the usability of application of the SFC in clinical diagnosis, we formed four categories of indices which are as follows: content/ concentration (9 indices), morphology (26 indices), age (5 indices), and function (8 indices). The erythrocytes of 39 healthy volunteers were analyzed with the SFC to fix the first-ever reference intervals for the new indices introduced. The essential measurable reliability of the presented method is expressed in terms of errors of characteristics of single erythrocytes retrieved from the solution of the inverse lightscattering problem and errors of parameters retrieved from the fitting of the experimental kinetics by molecular-kinetics model of erythrocyte lysis.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…where 𝑤(𝜉) is a Hann window function. Other window functions can also be used, for example, the Blackman-Nuttall window [18,20]. The numerical calculations are performed with the fast Fourier transform.…”

Section: Measured Signal and Fourier Transformationmentioning

confidence: 99%

“…Later these two parameters were tuned to obtain a solution in a wider range of characteristics [18]. The phase spectrum found applications in non-sphericity estimation [19] and classification [20]. Here we consider the use of the phase spectrum for characterization and start with the results obtained in Ref.…”

Section: Spectral Parametersmentioning

confidence: 99%

See 1 more Smart Citation

Retrieving the refractive index of a sphere from the phase spectrum of its light-scattering profile

Romanov

Yurkin

2021

J. Phys.: Conf. Ser.

View full text Add to dashboard Cite

We studied the Fourier spectrum of the light-scattering profiles of single particles in the Rayleigh-Gans-Debye (RGD) and Wentzel–Kramers–Brillouin (WKB) approximations. In the case of a homogeneous sphere, we found the relationship between the key parameters of the spectrum (including its phase) and the sphere characteristics – both analytically and numerically in the framework of the approximations and the rigorous Lorentz–Mie theory, respectively. Based on these results, we have improved the existing spectral characterization method for spheres extending the applicability range to particles with a higher refractive index.

show abstract

Ultraviolet light scattering scanning flow cytometry in the characterization of submicron microparticles

et al. 2023

Self Cite

View full text Add to dashboard Cite

Ultraviolet lasers are commonly used in flow cytometry to excite fluorochrome molecules with subsequent measurement of the specific fluorescence of individual cells. In this study, the performance of the ultraviolet light scattering (UVLS) in the analysis of individual particles with flow cytometry has been demonstrated for the first time. The main advantage of the UVLS relates to the improvement of the analysis of submicron particles due to the strong dependence of the scattering efficiency on the wavelength of the incident light. In this work, submicron particles were analyzed using a scanning flow cytometer (SFC) that allows measurements of light scattering in an angle‐resolved regime. The measured light‐scattering profiles of individual particles were utilized in solution of the inverse light‐scattering problem to retrieve the particle characteristics using a global optimization. The standard polystyrene microspheres were successfully characterized from the analysis of UVLS which provided the size and refractive index (RI) of individual beads. We believe that the main application of UVLS relates to the analysis of microparticles in a serum, in particular in the analysis of chylomicrons (CMs). We have demonstrated the performance of the UVLS SFC in the analysis of CMs of a donor. The RI versus size scatterplot of CMs was successfully retrieved from the analysis. The current set‐up of the SFC has allowed us to characterize individual CMs starting from the size of 160 nm that provides determination of the CM concentration in a serum with flow cytometry. This feature of the UVLS should help with the analysis of lipid metabolism measuring RI and size map evolution after lipase action.

show abstract

Spectral approach to recognize spherical particles among non-spherical ones by angle-resolved light scattering

Cited by 13 publications

References 38 publications

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

Retrieving the refractive index of a sphere from the phase spectrum of its light-scattering profile

Ultraviolet light scattering scanning flow cytometry in the characterization of submicron microparticles

Contact Info

Product

Resources

About