Spectral analysis and anti-bacterial activity of methanolic fruit extract of Citrullus colocynthis using gas chromatography-mass spectrometry

Abstract

Phytochemicals are chemical compounds often referred to as secondary metabolites. Thirty three bioactive phytochemical compounds were identified in the methanolic extract of Citrullus colocynthis. The identification of phytochemical compounds is based on the peak area, retention time molecular weight and molecular formula. Gas chromatography-mass spectrometry (GC-MS) analysis of C. colocynthis revealed the existence of the methyl 6-oxoheptanoate, hexanoic acid, 2-isopropyl-2-methyl-5-oxo-, methyl ester, dodecanoic acid, 3-hydroxy, benzofuran,2,3-dihydro, 1,1-Cyclopropanedimethanol, 2-methyl-α-phenyl, 1,1-cyclopropanedimethanol, 2-methyl-α-phenyl, 12,15-octadecadiynoic acid, methyl ester,pregnane-3,20ß-diol, 14α,18α-[4-methyl-3-oxo-(1-oxa-4-azabutan, 3-(N,Ndimethyllaurylammonio)propanesulfonate, 2H-1-benzopyran-3,4-diol,2-(3,4-dimethoxyphenyl)-3,4dihydro-6-met, 11,13-dihydroxy-tetradec-5-ynoic acid, methyl ester, Cyclopenta[1,3]cyclopropa[1,2]cycloheptan-3(3aH)-one,1,2,3b,6,7, 4-(2,4,4-trimethyl-cyclohexa-1,5dienyl)-but-3-en-2-one, 1-tetradecanamine,N,N-dimethyl, α-D-glucopyranoside, O-α-D-glucopyranosyl-(1,fwdarw.3)-ß-D-fructo, acetamide, N-methyl-N-[4-(3-hydroxypyrrolidinyl)-2-butynyl]-, 9octadecenamide,(z)- , butyrophenone,2´,3,4´,6´-tetramethyl-, ethyl 5,8,11,14,-eicosatetraenoate, 9,12,15octadecatrienoic acid, 2,3,-dihydroxypropyl ester, (Z,Z,Z) 5,7b,9,9a 476.241018tetrol,1a,1b,4,4a, 9,12,15-octadecatrienoic acid, 9,10-Secocholesta -5,7,10(19)triene-3,24,25,-triol,(3ß,5Z,7E)-, 9,12,15-Octadecatrienoic acid,2,3-dihydroxypropyl ester, (Z,Z,Z)-, triazido-(1,2,3,4,5-pentamethylcyclopenta-2,4-dienyl)-german, ethyl iso-allocholate, α-N-Normethadol, Octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, phthalic acid , decyl oct-3-yl ester, 1,2-Benzenedicarboxylic acid , bis(8-methylnonyl)ester, phthalic acid, di(6-ethyl-3-octyl)ester, y-tocopherol, 1,4-ethanonaphthalene -6,9(4H)-dione,1,4a,5,8a-tetrahydro-4,5,7,10 and vitamin E. Methanolic extract of bioactive compounds of C. colocynthis was assayed for in vitro antibacterial activity against Proteus mirabilis, Escherichia coli, Pseudomonas aerogenosa, P. mirabilis, Staphylococcus aureus and Klebsiella pneumonia by using the diffusion method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters of inhibition zones ranged from 4.91±0.260 to 1.03±0.200 mm for all treatments. -, 1H-cyclopropa[3,4]benz[1,2-e]ezulene -

GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod

“…The major constituents found were α‐N‐Normethadol (17.87 %, opioid), (Z,Z,Z)‐9,12,15‐Octadecatrienoic acid 2,3‐dihydroxypropyl ester, (14.02 %, fatty acid ester), l‐Gala‐l‐ido‐octonic lactone (10.59 %, essential oil), 9,12,15‐Octadecatrienoic acid 2‐[(trimethylsilyl)oxy]‐1‐[[(trimethylsilyl)oxy]methyl]ethyl ester, (Z,Z,Z) (9.88 %, fatty acid ester) and 1,2‐benzenedicarboxylic acid butyl 2‐methylpropyl ester (8.86 %, essential oil). The most prevailing component in the extract α ‐N‐Normethadol (17.87 %) is an opioid compound also reported previously in methanolic fruit extract of Citrullus colocynthis [29] . α‐N‐Normethadol is a bioactive compound reported to exhibit several pharmacological activities like anti‐bacterial, anti‐oxidant activity, anti‐inflammatory, and anti‐arthritic properties [30] .…”

“…The most prevailing component in the extract α -N-Normethadol (17.87 %) is an opioid compound also reported previously in methanolic fruit extract of Citrullus colocynthis. [29] α-N-Normethadol is a bioactive compound reported to exhibit several pharmacological activities like anti-bacterial, anti-oxidant activity, antiinflammatory, and anti-arthritic properties. [30] (Z,Z,Z)-9,12,15-Octadecatrienoic acid 2,3-dihydroxypropyl ester has anti-inflammatory and central nervous depressant activity.…”

Optimization and Characterization of Antimicrobial Agents Produced by Marine Aspergillus terreus She05 against Aeromonas hydrophila and other Applications

“…47 Antioxidant activity of Aspergillus species was also recognized in previous study. 48 Marine fungi accompanying with Halopteris scoparia (Linnaeus) Sauvageau has been proven as antioxidant producers. 21 Chemical characterization of ethyl acetate crude extract Five peaks were distinguished in ethyl acetate crude extract of A. terreus SHE05 as shown in GC-MS chromatogram (Fig.…”

Comparative Phytochemical Profiling (GC-MS and HPLC) and Evaluation of Antioxidant Activities of Wild, in Vitro Cultured and Greenhouse Plants of Kaempferia grandifolia Saensouk and Jenjitt and Kaempferia siamensis Sirirugsa; Rare Plant Species in Thailand

“…The compounds identified from extracts; neophytadiene, hexadecane, octadecanamide, lupeol, and betulinaldehyde 3,7,11,15-tetramethyl-2-hexadecen-1-ol are normally reported for their antimicrobial, anti-inflammatory, anticancer, antioxidant, antitumor, chemopreventive, lipoxygenase-inhibitor, and pesticidal abilities. [13][14][15][16][17] The Quantifications of the Phenolic Acid and Flavonoids by HPLC…”