Speckle correlation resolution enhancement of wide-field fluorescence imaging

Abstract

High-resolution fluorescence imaging is essential in nanoscience and biological sciences. Due to the diffraction limit, conventional imaging systems can only resolve structures larger than 200 nm. Here, we introduce a new fluorescence imaging method that enhances the resolution by using a high-index scattering medium as an imaging lens. Simultaneously, we achieve a wide field of view. We develop a new image reconstruction algorithm that converges even for complex object structures. We collect two-dimensional fluorescence images of a collection of 100 nm diameter dye-doped nanospheres, and demonstrate a deconvolved Abbe resolution of 116 nm with a field of view of 10 μm × 10 μm. Our method is robust against optical aberrations and stage drifts, and therefore is well suited to image nanostructures with high resolution under ambient conditions.

Lasing in quasi-periodic and aperiodic plasmon lattices

“…We note that the speckle patterns, even if seemingly random, in fact can be used for applications due to their autocorrelation function. For instance, large improvements to microscopy have recently been shown on the basis of using speckle [54][55][56]. Speckle provides a form of structured illumination microscopy where information is retrievable through the speckle autocorrelation that acts as point-spread function.…”

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

“…1(d), but other wave front shaping methods, computational methods, or a combination of the two can also be used. 27,28 The above rationale holds for waveguides with many degrees of freedom, up to the spatial and Fourier filtering effects associated with coupling into the waveguide. We might also add that the above concept really is a generalization of the 4f imaging setup; in the 4f setup, the first lens performs a physical Fourier transform of the object, and the second lens performs the inverse Fourier transform, which reconstitutes the image of the object; in the generalization, the waveguide performs some complex transformation and the wave front shaping/computation stage performs the inverse of the same transformation, again, reconstituting the image.…”

Analytical modeling of light transport in scattering materials with strong absorption

“…Over the years, however, the realization has arisen that spatial inhomogeneities that scatter light have advantageous properties and allow applications that are otherwise impossible, for instance, an optical diffuser or a high-numerical aperture objective [2][3][4]. While both the know-how of and the control over optics that strongly scatters light has greatly advanced [5][6][7][8][9][10], the state-of-the-art is much less developed regarding optical systems that also strongly absorb light (or even re-emit light of a different color), even though important application fields occur in this regime, for instance solid-state lighting [11][12][13], biomedical optics [14][15][16][17][18][19], or powder technology [20][21][22].…”