Specificity of mutations induced by carbon ions in budding yeast Saccharomyces cerevisiae

Matuo, Youichirou; Nishijima, Shigehiro; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Shimizu, Kikuo

doi:10.1016/j.mrfmmm.2006.07.001

Cited by 34 publications

(33 citation statements)

References 29 publications

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“…The incorporation of these modified nucleotides leads to mispairing, producing transversions (Tchou & Grollman 1993). Matuo et al (2006) reported and discussed the generation of transversions in wildtype strains of Saccharomyces cerevisiae irradiated with carbonion beams. This common feature between the NHEJ-defective mus-52 strain in Neurospora and wild-type S. cerevisiae will be discussed in the next section.…”

Section: Mechanisms Of Mutation Formationmentioning

confidence: 99%

The type of mutations induced by carbon-ion-beam irradiation of the filamentous fungus Neurospora crassa

Kazama

Inoue

et al. 2013

Fungal Biology

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Section: Mechanisms Of Mutation Formationmentioning

confidence: 99%

The type of mutations induced by carbon-ion-beam irradiation of the filamentous fungus Neurospora crassa

Kazama

Inoue

et al. 2013

Fungal Biology

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“…In previous studies, the highest mutation frequency obtained by ionbeam irradiation was associated with survival rates of 1 to 10 % in Saccharomyces cerevisiae Meyen ex E.C. Hansen (Matuo et al 2006) and Aspergillus oryzae (Ahlburg) Cohn (Toyoshima et al 2012). Based on this premise, we selected and used doses of 100, 150, 200, 250, 300 and 350 Gy; these doses had resulted in survival rates of between 0.2 and 36.8 % in our wild isolate of B. bassiana (Table 3).…”

Section: Resultsmentioning

confidence: 87%

Benomyl-resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams

et al. 2014

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Fungicides targeted at phytopathogens can be harmful to entomopathogenic fungi. The purpose of this study was to use ion-beam irradiation technology to produce benomyl-resistant mutants of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. After irradiation of conidia at 150 Gy, two mutant isolates, BB22 and BB24, were selected on media containing the fungicide. In an assay of vegetative growth, BB22 and BB24 were over 500 and 800 times more tolerant to benomyl, respectively, compared with the wild isolate. However, in an assay of conidial germination, neither mutant had increased tolerance compared with the wild isolate. Both mutant isolates also had increased tolerance to thiophanate-methyl during vegetative growth, but reduced tolerance to diethofencarb. A mutation was found at position 198 of the β-tubulin gene in the mutant isolates, with a substitution of glutamate for alanine (E198A). Ion beams have great potential as a tool to improve the traits of entomopathogenic fungi such as increasing tolerance to fungicides. Fungicide-resistant mutants produced in this way could be useful agents for biological control within IPM programmes where fungicides are also used.

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“…Heavy ion beams induce changes in miRNA expression levels and the specificity miRNAs in rice [4]. Carbon ions generated more than ten times the number of mutations induced by γ-rays and caused base changes, including transversions (G:C→T:A, 68.7%), transitions (G:C→A:T, 13.7%), and deletions/insertions (17.6%) in budding yeast strain [5]. Recently, heavy ion beams have been used as a novel and efficient method for tumor radiotherapy and mutagenesis in plant breeding [1].…”

mentioning

confidence: 99%

Induction of a High-Yield Lovastatin Mutant of Aspergillus terreus by 12C6+ Heavy-Ion Beam Irradiation and the Influence of Culture Conditions on Lovastatin Production Under Submerged Fermentation

Song

et al. 2011

Appl Biochem Biotechnol

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Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains. Furthermore, the main growth conditions as well as the influences of carbon and nitrogen sources on the growth and the lovastatin production of the mutant and the original strains were investigated comparatively. The spores of A. terreus CA99 were irradiated by 15, 20, 25, and 30 Gy of 80 MeV/u (12)C(6+) heavy-ion beams. Based on the lovastatin contents in the fermentation broth, a strain designated as A. terreus Z15-7 has been selected from the clone irradiated by the heavy-ion beam. When compared with the original strain, the content of lovastatin in the fermentation broth of A. terreus Z15-7 increased 4-fold. Moreover, A. terreus Z15-7 efficiently used the carbon and nitrogen sources for the growth and production of lovastatin when compared to the original strain. The maximum yield of lovastatin, 916.7 μg/ml, was obtained as A. terreus Z15-7 was submerged cultured in the chemically defined medium supplemented with 3% glycerol as a carbon source, 1% corn meal as an organic nitrogen source, and 0.2% sodium nitrate as an inorganic nitrogen source at 30 °C in the shake flask. The result shows that heavy-ion beam irradiation is an effective method for the mutation breeding of lovastatin production of A. terreus.

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Specificity of mutations induced by carbon ions in budding yeast Saccharomyces cerevisiae

Cited by 34 publications

References 29 publications

The type of mutations induced by carbon-ion-beam irradiation of the filamentous fungus Neurospora crassa

The type of mutations induced by carbon-ion-beam irradiation of the filamentous fungus Neurospora crassa

Benomyl-resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams

Induction of a High-Yield Lovastatin Mutant of Aspergillus terreus by 12C6+ Heavy-Ion Beam Irradiation and the Influence of Culture Conditions on Lovastatin Production Under Submerged Fermentation

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