Specificity of Chromosomal Changes Induced with X Rays in a Human T-Cell Line

Shiraishi, Yukimasa; Holdsworth, Robert N.; Minowada, Jun; Sandberg, Avery A.

doi:10.2307/3574949

Cited by 5 publications

(7 citation statements)

References 10 publications

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“…The much higher frequency of chromosomal aberrations seen in HSB2 suggests a slower or poorer repair of DNA strand breaks in this T-cell line. This reinforces our previous suggestions that the high sensitivity of HSB2 to radiation is due to the def ectiveness of repair mechanisms necessary to overcome the lesions produced by X-ray (Shiraishi et al 1976(Shiraishi et al , 1978Taguchi and Shiraishi 1983).…”

Section: Discussionsupporting

confidence: 78%

“…This suggests that a high frequency of chromatid exchanges can be an index of superior repair-ability of such normal and revertant cells, though chromatid exchanges themselves represent erroneous reunion of break points. Shiraishi et al (1978) reported an interesting observation using HSB2 that there is non-randomness in the distribution of X-ray-induced chromosome and chromatid breaks and their reunion between and within chromosomes or chromosome groups. Site-specific sensitivity of chromosomes to chemical agents including bleomycin in HSB2 remains to be studied.…”

Section: Discussionmentioning

confidence: 99%

“…The increased sensitivity of the human leukemic T-cell line (HSB2) to bleomycin, as well as to radiation (Shiraishi et al 1976(Shiraishi et al , 1978Taguchi and Shiraishi 1983), indicates that HSB2 resembles to ataxia telangiectasia cells in their susceptibility to chromosomal aberration-inducing treatments.…”

Section: Introductionmentioning

confidence: 99%

“…We have reported that this cell line is highly sensitive to radiation with regard to cell proliferation and chromosome damage, when compared with normal lymphocytes, a normal B-cell line and CCRF-HSB2-M which is a radiation-resistant mutant clone derived from HSB2 (Shiraishi et al 1976(Shiraishi et al , 1978Taguchi and Shiraishi 1983).…”

Section: Introductionmentioning

confidence: 99%

“…Considering the high sensitivity of HSB2 to radiation (Shiraishi et al 1976(Shiraishi et al , 1978Taguchi and Shiraishi 1983), it is of interest to examine the sensitivity of this cell line to bleomycin. The present paper demonstrates hypersensitivity of HSB2 to bleomycin in terms of a reduction in cell viability and an increase in chromosomal aberrations.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of bleomycin-induced chromosome damage in a human leukemic T-cell line.

Taguchi

1987

The Japanese journal of genetics

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A sensitivity to bleomycin was examined in a human leukemic T-cell line (CCRF-HSB2) established from the peripheral blood of a patient with acute lymphoblastic leukemia. At all concentrations of bleomycin tested, HSB2 showed reduced survival and increased chromosome breakage.A normal Bcell line (KS86) and a radiation-insensitive CCRF-HSB2-M cell line were found to be resistant against the effects of bleomycin. The increased sensitivity of the human leukemic T-cell line (HSB2) to bleomycin, as well as to radiation (Shiraishi et al. 1976(Shiraishi et al. , 1978Taguchi and Shiraishi 1983), indicates that HSB2 resembles to ataxia telangiectasia cells in their susceptibility to chromosomal aberration-inducing treatments.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhancement of bleomycin-induced chromosome damage in a human leukemic T-cell line.

Taguchi

1987

The Japanese journal of genetics

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Distribution of radiation induced lesions in human chromosomes and dose-effect relation analysed with G-banding

Bauchinger

Götz

1979

Radiat Environ Biophys

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Human female lymphocytes were exposed to X-rays in vitro at 7 different doses between 40-280 R. In 830 metaphases chromosome analyses were carried out with either conventional staining or G-banding, respectively. 486 breakpoints are non-randomly distributed between chromosomes and chromosome arms. An excess of lesions was present in chromosomes 1 and 5 or in 1p. 85% of the lesions were located in G-negative bands (pale G-bands). 29% of all lesions appeared in either the last terminal pale band (21%) or in the centromere region (8%). With regard to an application of G-banding for a biological dose-estimation, the dose-response relations of dic and ace were analysed. Although G-banding enables detailed analysis of the whole karyotype it cannot be recommended for cytogenetic routine analyses in medical radioprotection monitoring, without suitable automated scoring techniques. Dose estimations based on the frequency of dic and carried out with conventional staining cannot be essentially improved at present with banding. Nevertheless, by banding criteria for a correct evaluation of other aberration types, e.g. ace, can be provided. This is a prerequisite for the calculation of representative dose-effect curves.

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Inter- and intra-chromosomal distribution of chromatid breaks induced by X-rays during G2 in human lymphocytes

Pelliccia¹,

Micheli²,

Olivieri³

1985

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Specificity of Chromosomal Changes Induced with X Rays in a Human T-Cell Line

Cited by 5 publications

References 10 publications

Enhancement of bleomycin-induced chromosome damage in a human leukemic T-cell line.

Enhancement of bleomycin-induced chromosome damage in a human leukemic T-cell line.

Distribution of radiation induced lesions in human chromosomes and dose-effect relation analysed with G-banding

Inter- and intra-chromosomal distribution of chromatid breaks induced by X-rays during G2 in human lymphocytes

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