Enhancement of bleomycin-induced chromosome damage in a human leukemic T-cell line.

Abstract

A sensitivity to bleomycin was examined in a human leukemic T-cell line (CCRF-HSB2) established from the peripheral blood of a patient with acute lymphoblastic leukemia. At all concentrations of bleomycin tested, HSB2 showed reduced survival and increased chromosome breakage.A normal Bcell line (KS86) and a radiation-insensitive CCRF-HSB2-M cell line were found to be resistant against the effects of bleomycin. The increased sensitivity of the human leukemic T-cell line (HSB2) to bleomycin, as well as to radiation (Shiraishi et al. 1976(Shiraishi et al. , 1978Taguchi and Shiraishi 1983), indicates that HSB2 resembles to ataxia telangiectasia cells in their susceptibility to chromosomal aberration-inducing treatments.

Sister chromatid exchanges and chromosome aberrations in lymphocytes of nurses handling antineoplastic drugs

“…Many widely used anti-neoplastic drugs have been shown to be mutagens in bacteria (IARC, 1981;La Iglesia et al, 1984), in mammalian in vitro (Taguchi, 1987;Shinkai et al, 1988;Chibber and Ord, 1989) and in vivo assays (Tominaga et al, 1986;Singh and Gupta, 1988;Zhang et al, 1988). Therefore there is concern as to whether the handling of these agents may present an occupational health hazard.…”