Specificity of a mouse monoclonal antibody raised against acute myeloid leukaemia cells for mast cells in human mucosal and connective tissues

Abstract

Summary.A mouse monoclonal antibody raised against acute myeloid leukaemia cells (YB5.B8 monoclonal antibody; Gadd. S, J. and Ashman, L, K, (1985): Leukaemia Res. 9, 1329-1336) ha,s been found by an indirect immunoperoxidase lechnique to bind lo scattered cells in frozen sections from a number of buman tissues. They have been identified as mast cells in fixed sections of skin, tonsil and duodenum by simultaneous staining of glycosaminoglycan wilh Alcian blue in 0.7 N HCl, The antibody does not distinguish mast cells in mucosal tissues from Ihose in conneclive tissue, altbough ihe level of expression by cells al both sites appears to be heterogeneous. Wilh tbe exception of tow affinity binding lo B lymphocytes, no other bone marrow-derived cells were found to bind the antibody. In particular, basophils and eosinophils were not stained, suggesting that they are not related closely to masi cells and that the antigen detected by YB5.B8 monoclonal aniibody is not an IgE Fc receptor. Therefore, among all mature haemopoieiic lineages, the antibody is specific for mast cells.

c-kit-Immunopositive vascular progenitor cells populate human coronary in-stent restenosis but not primary atherosclerotic lesions

“…However, for the normal coronary artery cross sections, c-kit was expressed in a few adventitial cells that also expressed mast cell tryptase. Inasmuch as c-kit is normally expressed by mast cells, this is an expected finding and served as a positive control that argues against the possibility of false-negative immunolabeling results in the intima and media of these specimens (17). In contrast, all 13 ISR tissue specimens and 4 of 6 RS lesions expressed c-kit (Figs.…”

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

“…Erythrocytes were removed by centrifugation through a 65% continuous Percoll gradient (1.084 g/ml) and T lymphocytes by serial affinity selection using anti-CD2-coated magnetic beads (Dynabeads M-450 Pan-T; Dynal Biotech, Oslo, Norway), a technique which removes Ͼ99% of T lymphocytes. Nucleated cells were then incubated with the anti-c-kit mAb YB5.B8 (13,14) (donated by Dr. L. K. Ashman, Institute of Medical and Veterinary Science, Adelaide, South Australia), washed, and subjected to positive magnetic affinity using goat anti-mouse IgG-coated Dynabeads (15). This procedure yielded mast cells, identified by the mAb AA1 against tryptase (16), in purities of Ͼ95%.…”

Transformation of NIH3T3 fibroblasts by the c-Kit receptor tyrosine kinase: effect of receptor density and ligand-requirement

“…During haemopoiesis the c-KIT receptor is lost as progenitor cells di erentiate into more mature lineage-committed cells, except for mast cells which retain very high levels of c-KIT expression (Mayrhofer et al, 1987;Irani et al, 1992;Galli et al, 1994). Due to a disruption in haemopoiesis, patients with acute myeloid leukaemia (AML) accumulate within their bone marrow blast cells which have been blocked in the di erentiation process, frequently at a stage still retaining c-KIT expression.…”