Specificity in RNA Processing Reactions in Escherichia Coli

Apirion, David; Dallmann, Géza; Gurevitz, Michael; Miczák, András; Szeberenyi, Jozeff; Tomcsányi, T; Subbarao, M N; Watson, Ned

doi:10.1007/978-3-642-73139-6_7

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Bacterial and phage strains E.coli RNase E+ and E-isogenic strains N3433 (rne') and N3431 (me-3071 Ts) (Goldblum and Apirion, 1981), were obtained from B. Bachmann at the E. coli Genetic Stock Center (Yale University, USA). The RNase P-deficient (rnpA49) strain N2020 (Apirion, 1980) was from M. Belfort. U.Schmeissner provided the RNase MI+ and III isogenic pair A0159 mclO5 and A0160 rnc+ (Schmeissner et al, 1984); these had been constructed by A.Oppenheim from the rnclO5 mutant of Studier (Studier, 1975) in the SA500 (Adhya et al, 1974) background.…”

Section: Methodsmentioning

confidence: 99%

Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.

et al. 1988

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Gene 32 from bacteriophage T4 is transcribed as precursor transcripts which are processed to a stable product. This processing of the gene 32 mRNA was observed in RNase III or P‐deficient strains of Escherichia coli. However, after infection of an RNase E‐deficient strain, the amount of processed transcript was significantly reduced while the levels of the precursor transcripts remained high. RNase E therefore appears to have an essential role in the processing of the gene 32 mRNA. We have mapped the exact 5′ end of the processed transcript by primer extension. The cleavage occurs near a stem‐loop structure at a site which shows some similarity to other known RNase E cleavage sites. The effects of the processing on the differential stability of the upstream and downstream sequences, and on gene expression, are discussed.

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Section: Methodsmentioning

confidence: 99%

Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.

et al. 1988

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“…The strains used were N2076 (rnc'), N20777 ( m c ) [12]; N2018 (mc, r p ) [13]; N3589 is an rnc me strain like N3520 [14] [15]. These strains were infected with the T4427 deletion mutant [16] at a multiplicity of 15 -20 phages per bacterium.…”

Section: Bacterial Strainsmentioning

confidence: 99%

The ribonuclease-III-processing site near the 5′ end of an RNA precursor of bacteriophage T4 and its effect on termination

Gurevitz

Apirion

2008

European Journal of Biochemistry

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Infection of RNase III− (rnc) Escherichia coli cells with bacteriophage T4Δ27, a deletion mutant missing seven out of the ten genes in the tRNA transciption unit, results in the accumulation of tRNA precursor (10.5‐SRNA) that contains the sequence of tRNAGln, tRNALeu and species 1 RNA [Pragai and Apirion (1981) J. Mol. Biol. 153, 619–630]. In vitro studies, using partially purified RNase III or cell extracts and 10.5‐S RNA as substrate, have revealed a cleavage site at the 5′ side of the molecule. A computerized secondry structure suggests that the RNase III cleavage site can be placed in a small bulge which could be part of a duplex structure and is adjacent to A‐A‐G and its complementry sequence U‐U‐U in the same relative relationship found for most RNase III cleavage sites were the adjacent sequences are (A‐A‐G/U‐U‐C). Under normal processing conditions (presence of RNase III) the 3′ end of the processed intermediate precursors, 10.1‐S and p2Sp1 RNAs, is C‐U‐U‐(U1–2)‐UOH, which is determined by a stem and loop structure that could serve as a rho‐independent termination single site. However, in the absence of RNase III, the accumulated 10.5‐S precursor RNA does not terminate at the same site and its 3′ end is shifted a few nucleotides downstream. Thus, RNase III, besides playing a role in processing of 10.5‐S RNA, also affects the termination of that molecule, even though both sites, the RNase III cleavage site and the termination site, are about 390 nucleotides apart.

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RNA processing in prokaryotic cells

Apirion

Miczák

1993

BioEssays

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RNA processing in Escherichia coli and some of its phages is reviewed here, with primary emphasis on rRNA and tRNA processing. Three enzymes, RNase III, RNase E and RNase P are responsible for most of the primary endonucleolytic RNA processing events. The first two are proteins, while RNase P is a ribozyme. These three enzymes have unique functions and in their absence, the cleavage events they catalyze are not performed. On the other hand a relatively large number of exonucleases participate in the trimming of the 3' ends of tRNA precursor molecules and they can substitute for each other. Primary processing is the first event that happens to the nascent RNA molecule, while in secondary RNA processing, the substrate is a product of a primary processing event. Although most RNA processing occurs in RNP particles, it seems that only in secondary RNA processing is the RNP particle required for the reaction. Bacteria and especially bacteriophages contain self-splicing introns which in cases were probably acquired from other species.

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Specificity in RNA Processing Reactions in Escherichia Coli

Cited by 3 publications

References 25 publications

Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.

Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.

The ribonuclease-III-processing site near the 5′ end of an RNA precursor of bacteriophage T4 and its effect on termination

RNA processing in prokaryotic cells

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