Specificity determinants for Cry insecticidal proteins: Insights from their mode of action

Jurat-Fuentes, Juan Luís; Crickmore, N.

doi:10.1016/j.jip.2016.07.018

Cited by 149 publications

(112 citation statements)

References 69 publications

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“…In recent years, the trend is to include multiple genes of interest within a single DNA construct (Weber et al, 2012;NAS, 2016). For example, for effective insect control, different mechanisms of action can be identified by a combination of competitive binding experiments and testing new control agents against insects resistant to other insecticides (Pardo-López et al, 2013;Ladics et al, 2015aLadics et al, , 2015bChakroun et al, 2016;Jerga A et al, 2016;Jurat-Fuentes and Crickmore, 2017;Moar et al, 2017). Typically, dozens of versions of an expression cassette are tested in plants, both in greenhouse and confined field trials, to determine which best optimizes the gene expression needed to ensure commercial viability and reproducible efficacy of the trait.…”

Section: Sources Of Candidate Genes For Gm Crops and Early Testingmentioning

confidence: 99%

Bringing New Plant Varieties to Market: Plant Breeding and Selection Practices Advance Beneficial Characteristics while Minimizing Unintended Changes

et al. 2017

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Commercial‐scale plant breeding is a complex process in which new crop varieties are continuously being developed to improve yield and agronomic performance over current varieties. A wide array of naturally occurring genetic changes are sources of new characteristics available to plant breeders. During conventional plant breeding, genetic material is exchanged that has the potential to beneficially or adversely affect plant characteristics. For this reason, commercial‐scale breeders have implemented extensive plant selection practices to identify the top‐performing candidates with the desired characteristics while minimizing the advancement of unintended changes. Selection practices in maize (Zea mays L.) breeding involve phenotypic assessments of thousands of candidate lines throughout hundreds of different environmental conditions over many years. Desirable characteristics can also be introduced through genetic modification. For genetically modified (GM) crops, molecular analysis is used to select transformed plants with a single copy of an intact DNA insert and without disruption of endogenous genes. All the while, GM crops go through the same extensive phenotypic characterization as conventionally bred crops. Data from both conventional and GM maize breeding programs are presented to show the similarities between these two processes.

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Section: Sources Of Candidate Genes For Gm Crops and Early Testingmentioning

confidence: 99%

Bringing New Plant Varieties to Market: Plant Breeding and Selection Practices Advance Beneficial Characteristics while Minimizing Unintended Changes

et al. 2017

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“…They exhibit specific activity against one or several orders of insects belonging to the orders Lepidoptera, Diptera, and Coleoptera [22,25,26], mainly due to the specificity of membrane receptors [27,28]. These receptors are absent in beneficial insects, plants, and mammals [26,29].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria

Djenane

Natèche

Amziane

et al. 2017

Toxins

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This work represents the first initiative to analyze the distribution of B. thuringiensis in Algeria and to evaluate the biological potential of the isolates. A total of 157 isolates were recovered, with at least one isolate in 94.4% of the samples. The highest Bt index was found in samples from rhizospheric soil (0.48) and from the Mediterranean area (0.44). Most isolates showed antifungal activity (98.5%), in contrast to the few that had antibacterial activity (29.9%). A high genetic diversity was made evident by the finding of many different crystal shapes and various combinations of shapes within a single isolate (in 58.4% of the isolates). Also, over 50% of the isolates harbored cry1, cry2, or cry9 genes, and 69.3% contained a vip3 gene. A good correlation between the presence of chitinase genes and antifungal activity was observed. More than half of the isolates with a broad spectrum of antifungal activity harbored both endochitinase and exochitinase genes. Interestingly, 15 isolates contained the two chitinase genes and all of the above cry family genes, with some of them harboring a vip3 gene as well. The combination of this large number of genes coding for entomopathogenic proteins suggests a putative wide range of entomotoxic activity.

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“…However, evolution of pest resistance to Bt crops is rapidly increasing . The primary mechanism of resistance involves mutations that disrupt midgut protein receptors and reduce the binding of Bt toxins . Such genetic changes are expected to compromise the normal functions of the affected midgut proteins, thereby imposing fitness costs that select against resistance in habitats where Bt toxins are absent .…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5] The primary mechanism of resistance involves mutations that disrupt midgut protein receptors and reduce the binding of Bt toxins. [6][7][8] Such genetic changes are expected to compromise the normal functions of the affected midgut proteins, thereby imposing fitness costs that select against resistance in habitats where Bt toxins are absent. [9][10][11][12][13][14][15][16] Knowledge of such costs can enhance the development of proactive resistance management strategies and remedial actions to address resistance evolution.…”

Section: Introductionmentioning

confidence: 99%

Genotype‐specific fitness cost of resistance to Bt toxin Cry1Ac in pink bollworm

Carrière

Williams²,

Crowder

et al. 2018

Pest Management Science

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Specificity determinants for Cry insecticidal proteins: Insights from their mode of action

Cited by 149 publications

References 69 publications

Bringing New Plant Varieties to Market: Plant Breeding and Selection Practices Advance Beneficial Characteristics while Minimizing Unintended Changes

Bringing New Plant Varieties to Market: Plant Breeding and Selection Practices Advance Beneficial Characteristics while Minimizing Unintended Changes

Assessment of the Antimicrobial Activity and the Entomocidal Potential of Bacillus thuringiensis Isolates from Algeria

Genotype‐specific fitness cost of resistance to Bt toxin Cry1Ac in pink bollworm

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