Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Abstract

A group of monoclonal antibodies to the staphylococcal enterotoxins B and C1 without any cross-reactivity to the other known staphylococcal enterotoxins A, C2, C3, D and E was developed. The monoclonal antibodies were compared in competition ELISA's with regard to affinity and epitope recognition. Three different groups could be classified: Group 1, consisting of the MAbs B/3-4 and B/3-8, recognizes in identical manner staphylococcal enterotoxin B. Also group 2 (MAb B/3-5) recognizes only staphylococcal enterotoxin B, yet group 1 and 2 do not compete with each other in the competition ELISA and therefore own different paratopes. Group 3 (MAb C1/2-3 and C1/4-6) reacts exclusively with staphylococcal enterotoxin C1, not even with staphylococcal enterotoxins C2 and C3.

Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B

“…Particularly noticeable was that many of the SEB-selected Fabs cross-reacted with SEC1 and/or SEC2. Similar results were previously demonstrated with rabbit antisera [9], [52] and mouse monoclonal antibodies [7], [53]. SEB shares the highest sequence identity with SECs (68%), versus SEA (33%) or TSST-1 (26%) [54], [55].…”

Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization

“…Absorbances in each case (IgG~ or IgG2b) were markedly higher than in other subclasses (data not shown). The percentage of mAbs belonging to IgGzb subclass (42.85%) is much higher than those described by other authors for SEs [2,9,17] and most authors describe antibodies belonging only to lgG 1 subclass [6,10,14].…”

“…There are many reports on the development and use of monoclonal antibodies (mAbs) produced against SEs [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. mAbs to all of the identified enterotoxins have been prepared [2], either specific or cross-reactive.…”

Development of serum IgM antibodies against superantigens ofStaphylococcus aureusandStreptococcus pyogenesin Kawasaki disease

“…These disparities of kind and number of SAgs involved may be due to the following differences; ages of control subjects, immunological classes in analyses, ELISA methods, the timing of blood collection, and geographical populations. Alternatively, because of molecular mimicry between SEB, SEC, and SPEA [17,18], crossreacted IgG or IgM antibodies [19,20] might be detected in our study. In any case, the specific organism may not be as important as a common mechanism of stimulation to develop the immune system.…”