Specific nutrient uptake during initiation of somatic embryogenesis in coconut calluses

Dussert, Stéphane; Verdeil, Jean‐Luc; Buffard-Morel, Jacqueline

doi:10.1016/0168-9452(95)04248-s

Cited by 21 publications

(10 citation statements)

References 16 publications

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“…Thereafter, the formation of a meristematic zone was observed, very similar to those described in coconut primary callus (Dussert et al 1995;Fernando et al 2003), and this was associated with primary callus development.…”

Section: Discussionmentioning

confidence: 54%

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Somatic embryogenesis from peach palm zygotic embryos

Steinmacher

Cangahuala-Inocente

Clement

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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The factors affecting the induction and development of somatic embryos and plantlet acclimatization of peach palm (Bactris gasipaes Kunth) were evaluated to establish an efficient regenerative protocol based on somatic embryogenesis. Mature zygotic embryos were cultured in Murashige and Skoog (MS) medium supplemented with 0-40 μM of picloram (4-amino-3,5,6-trichloropicolinic acid) and 0 or 5 μM of 2-isopentyladenine (6-dimethylaminopurine) (2-iP). After 5 mo. in culture embryogenic callus arose from primary calli. Picloram (10 μM) was effective in inducing embryogenic calli in 9.8% of the explants. The use of 1 μM of AgNO 3 enhanced embryogenic competence. Embryogenic calli showed an organized structure, a globular aspect, and were white to yellowish in color. Histological analyses showed that cell proliferation arose from subepidermal cells adjacent to vascular bundles, resulting in primary callus formed by a meristematic zone from which somatic embryos arose. Protein profile analyses revealed two high molecular mass bands in these embryogenic calli, but not in other tissues. Embryogenic calli were transferred to a culture medium containing 40 μM of 2,4-dichlorophenoxyacetic acid, 10 μM of 2-iP, plus 1 g l −1 of glutamine, hydrolyzed 0.5 g l −1 casein, and activated 1.5 g l −1 of charcoal. Morphogenetic responses achieved in this medium were the development of somatic embryos, rooting, and loss of embryogenic capacity. Somatic embryos were converted to plantlets on MS medium plus 24.6 μM of 2-iP and 0.44 μM of naphthalene acetic acid. Plantlets were maintained in MS medium with activated charcoal (1.5 g l −1 ) until they were 6 cm tall, and then acclimatized. After 16 wk, 84.2±6.4% survival was observed.

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Section: Discussionmentioning

confidence: 54%

“…These somatic embryos originated from the meristematic zone, following the multicellular pattern (Williams and Maheswaran 1986). In coconut, somatic embryos of unicellular (Dussert et al 1995) or multicellular (Fernando et al 2003) origin were described arising from the meristematic zone.…”

Section: Discussionmentioning

confidence: 97%

Somatic embryogenesis from peach palm zygotic embryos

Steinmacher

Cangahuala-Inocente

Clement

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…The initiation of somatic embryogenesis in Cocos nucifera was increased by the uptake of magnesium [53]. Earlier scientists also observed that a reduction in the concentration of macronutrients in MS medium suppressed the growth of embryogenesis [20].…”

Section: Discussionmentioning

confidence: 95%

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

Saha

Bandyopadhyay

Raychaudhuri

2010

Biol Trace Elem Res

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A nutrient medium has been formulated by altering the macro- and micro-elemental concentration in the culture medium for in vitro somatic embryo induction of economically important medicinal plant Plantago ovata Forsk .A comparison was made between induced embryos with normal embryos (produced in Murashige and Skoog (MS) medium) to observe frequency of embryo induction and also to determine regeneration efficiency. In the present investigation, three different media have been formulated. Among them, FM3 (formulated media, treatment 3) was the most suitable for increasing the frequency of somatic embryo production and regeneration of P. ovata Forsk. Better result was obtained using formulated medium than with MS medium.

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“…Due to the importance of Ca, K and Mg in some biochemical processes, [12][13][14] their quantification is of utmost importance for the understanding of these processes. In this way, efficient methods of sample preparation, mainly those presenting accuracy and fastness, are necessary for appropriate metal quantification.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

Arruda¹,

Rodriguez²,

Arruda³

2003

J. Braz. Chem. Soc.

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Apresenta-se um procedimento para extração de Ca, K e Mg de amostras de cultivo in vitro de plantas, comparando-se culturas de calo com diferentes potenciais embriogênicos de Citrus sinensis e Citrus limonia, com o emprego de energia ultra-som. Foram investigados os parâmetros relacionados com a extração de metais, tais como tipo de amostragem do material, concentração do ácido e tempo de sonicação. Visando a comprovação da exatidão, o procedimento de extração ultra-sônica foi comparado com o de digestão ácida assistida por microondas, e não foram observadas diferenças estatísticas significativas em um nível de confiança de 95%. Com este procedimento de extração simples e preciso, foi possível estabelecer diferenças nas concentrações de Ca, K e Mg durante a formação/desenvolvimento dos embriões de Citrus, bem como entre as culturas (embriogênicas e não-embriogênicas). Finalmente, a extração ultra-sônica demonstrou-se uma excelente alternativa para menor manipulação das amostras e redução de custos operacionais.An ultrasound extraction procedure for Ca, K and Mg from in vitro plant cultures is proposed, comparing cultures of different embryogenic levels of Citrus sinensis and Citrus limonia, employing ultrasound energy. Parameters related to metals extraction, such as plant material sampling, acid concentration and sonication time were investigated. For accuracy check, the proposed ultrasound extraction procedure was compared with a microwave-assisted digestion procedure and no differences in the results were verified at 95% of the confidence level. With this simple and accurate extraction procedure, it was possible to determine differences in Ca, K and Mg concentrations during Citrus embryo formation/development and between cultures (embryogenic and non-embryogenic). Finally, the ultrasound extraction method demonstrated to be an excellent alternative for handless sampling and operational costs. Keywords: ultrasound extraction, FAAS, FAES, Ca, K and Mg determination IntroductionClassical sample preparation methods commonly used for biological studies are those based on dry or wet decomposition, 1,2 which, in general, require intensive sample handling and time/reagent consumption. In this context, the use of ultrasound can be an excellent alternative to minimize sample preparation [3][4][5] and to avoid solvent extraction, 6,7 because its energy facilitates and accelerates some analytical steps, permitting less time consumption and decreasing the cost per analysis. Besides these characteristics, accuracy, precision, and handless manipulation can be achieved when ultrasound-assisted extraction methods are used. 4 As an example, the application of ultrasound as an auxiliary energy source for solid sample treatment was made by Luque de Castro and Silva. 8 The authors emphasized different applications of ultrasound mainly related to sample treatment of agricultural, biological and environmental samples. Carvalho et al. 9 pointed out the sonochemical degradation that occurs inside extracted solutions when using the ...

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Specific nutrient uptake during initiation of somatic embryogenesis in coconut calluses

Cited by 21 publications

References 16 publications

Somatic embryogenesis from peach palm zygotic embryos

Somatic embryogenesis from peach palm zygotic embryos

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

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