Specific Induction of Metallothionein in Hairless Mouse Skin by Zinc and Dexamethasone

Mullin, Catherine H.; Frings, Gudrun; Abel, Josef; Kind, Peter; Goerz, G

doi:10.1111/1523-1747.ep12470553

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…24 MT up-regulation can be induced in vivo by exposure to zinc. 25 Collectively, all of these observations argue that the requirement for zinc is higher in skin during the process of healing. Improvement in wound healing on exposure to zinc is not limited to oral supplementation: topically applied zinc compounds are also effective.…”

Section: Clinical Observations: Evidence For the Importance Of Zincmentioning

confidence: 99%

“…Finally, expression of MT, which serves as a reservoir of zinc, is higher in healing wounds than in normal skin 23 ; treatment of keratinocytes in vitro with a zinc chelator [tetrakis‐(2‐pyridylmethyl) ethylenediamine] inhibits the up‐regulation of MT and also cellular proliferation 24 . MT up‐regulation can be induced in vivo by exposure to zinc 25 . Collectively, all of these observations argue that the requirement for zinc is higher in skin during the process of healing.…”

Section: Role Of Zinc In Skin Physiologymentioning

confidence: 99%

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Zinc and Skin Health

Schwartz

Marsh²,

Draelos³

2005

Dermatologic Surgery

111

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Section: Clinical Observations: Evidence For the Importance Of Zincmentioning

confidence: 99%

Section: Role Of Zinc In Skin Physiologymentioning

confidence: 99%

Zinc and Skin Health

Schwartz

Marsh²,

Draelos³

2005

Dermatologic Surgery

111

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show abstract

“…Immunohistochemical staining with monoclonal reactive antibodies reveals that, while the genes of the isoform IV are expressed in the cornified and stratified epithelium of the skin, tongue and upper part of the alimentary tract (2), those of MT-I and -II occurs in some skin tumors, following exposure to glucocorticoids, to metallic and nonmetallic compounds, or in oxidative stress and inflammatory processes (5)(6)(7)(8). Van deer Oord & De Ley (4), studying the expression and distribution of MT in frozen sections of normal and pathological human skin, showed that the genes of MT-I and II were expressed in the basal keratinocytes of the epidermis, outer hair root sheath, hair matrix cells, secretory coils of eccrine glands and myoepitelial cells around apocrine sweat glands of normal fetal and adult skin.…”

mentioning

confidence: 99%

Expression of metallothioneins‐I and ‐II isoforms at positive patch‐test sites

et al. 2000

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The expression and the distribution of metallothioneins (MT)-I and II isoforms were evaluated in 5 healthy volunteers and in 16 subjects with positive patch test reactions to various compounds. Skin specimens taken both from the healthy skin of the back and at positive patch test sites (at 48 h), were treated using a 3-step indirect immunoperoxidase procedure with a mouse monoclonal IgG1 antibody reactive against I and II isoforms of human, rat and horse MT. MT were expressed in the basal layer of the healthy skin of both controls and sensitive subjects, without any significant difference. At positive patch test sites, there was an overexpression of MT in basal and suprabasal layers of the epidermis. Overexpression of MT, related to the degree of the inflammatory reactions elicited by the penetrating compounds was observed in the dermis. The cells expressing MT in the dermis were mostly T lymphocytes and cells with dendritic morphology which positively stained in part for CD34 and in part for XIIIa markers and negatively for KP1, S100 and HLA-DR. Taken together, these results seem to indicate that MT represent a constitutive mechanism of defence expressed by different types of cells in the skin, which is triggered by contact with both metallic and non-metallic compounds. The biological significance of MT in the skin remains to be elucidated. Our preliminary findings do not permit evaluation of whether these nearly ubiquitous proteins exert their cytoprotective effects in the skin acting simultaneously as antioxidant, metal binding or zinc suppliers, or if they display these activities mainly depending on the nature of the penetrating substances.

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