Specific in vitro antibody responses by human blood lymphocytes: apparent nonresponsiveness of PBL is due to a lack of recirculating memory B cells.

Callard, Robin E.; McCaughan, G.W.; Babbage, J; Souhami, Robert L.

doi:10.4049/jimmunol.129.1.153

Cited by 22 publications

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In Vitro Response to HBsAg of Peripheral Blood Lymphocytes from Recipients of Hepatitis B Vaccine

Chang¹,

Celis²,

Miller³

et al. 1984

Hepatology

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Lymphocytes isolated from recipients of hepatitis B vaccine were studied for their immune response to HBsAg in vitro. Peripheral blood mononuclear cells (PBMs) from 70 to 80% of 40 vaccinees yielded proliferative indices larger than 2 after 5 to 7 days incubation with HBsAg. This in vitro proliferative response could be augmented by incubating the cells with HBsAg and supernatants of activated T cells for 2 weeks or longer. After 7 to 10 days, in vitro stimulation with antigen, PBMs (1 X 10(6] could yield 5 to 15 HBsAg-specific antibody-secreting plaque-forming cells. The antibody to HBsAg produced in vitro was greatly increased in cultures that contained antigen-specific B cells enriched by panning with HBsAg-coated plates and a T cell growth factor-dependent, HBsAg-specific autologous T cell line. The results indicate that HBsAg-specific B and T cells are present, although at low frequencies, in the circulation of hepatitis B vaccinees.

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In Vitro Response to HBsAg of Peripheral Blood Lymphocytes from Recipients of Hepatitis B Vaccine

Chang¹,

Celis²,

Miller³

et al. 1984

Hepatology

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A prospective study of in vitro anti‐HBs producing B cells (spot‐ELISA) following primary and supplementary vaccination with a recombinant hepatitis B vaccine in insulin dependent diabetic patients and matched controls

Wismans¹,

Hattum²,

Gast³

et al. 1991

Journal of Medical Virology

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A prospective study of the immune response after hepatitis B vaccination was carried out in 32 insulin dependent diabetes mellitus (IDDM) patients and their age and sex matched healthy controls. A sensitive, immunoenzymatic technique was used, able to detect in vitro specific antibody production by mitogen stimulated individual B cells. In-vivo serologic response after vaccination with a standard scheme (0, 1 and 6 months) of 20 micrograms recombinant hepatitis B (HB) vaccine was significantly impaired in the IDDM patients both with respect to the number of nonresponders (25 versus 3%, P less than 0.05) and antibody titers reached (1,377 vs. 9,060 IU/L, P less than 0.05). The total number of in vitro IgM- and IgG-class immunoglobulin producing B cells as detected by the spot-ELISA, was found to be comparable in both groups. Specific IgG anti-HBs (and to a lesser extent IgM anti-HBs) showed impairment in the diabetic population as a whole. The number of IgG anti-HBs producing B cells was markedly depressed one month following vaccination, which is probably a reflection of homing of B cells outside the circulation. Responding subjects were identified early during their vaccination by the detection of in vitro anti-HBs production using the spot-ELISA. Non-responding healthy subjects and IDDM patients as a group showed a low number of IgG anti-HBs spots, suggesting a reduced specific memory B cell frequency. In 13 of 15 hypo- and nonresponders with positive IgG anti-HBs spots supplementary vaccination(s) resulted in improved anti-HBs levels.

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Production of Human Monoclonal Antibodies

Buck¹,

Larrick²,

Raubitschek³

et al. 1984

Monoclonal Antibodies and Functional Cell Lines

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Specific in vitro antibody responses by human blood lymphocytes: apparent nonresponsiveness of PBL is due to a lack of recirculating memory B cells.

Cited by 22 publications

References 0 publications

In Vitro Response to HBsAg of Peripheral Blood Lymphocytes from Recipients of Hepatitis B Vaccine

In Vitro Response to HBsAg of Peripheral Blood Lymphocytes from Recipients of Hepatitis B Vaccine

A prospective study of in vitro anti‐HBs producing B cells (spot‐ELISA) following primary and supplementary vaccination with a recombinant hepatitis B vaccine in insulin dependent diabetic patients and matched controls

Production of Human Monoclonal Antibodies

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