Production of Human Monoclonal Antibodies

Buck, David; Larrick, James W.; Raubitschek, Andrew; Truitt, Kenneth E.; Senyk, George; Wang, J.; Dyer, Bradley J.

doi:10.1007/978-1-4684-4673-9_11

Cited by 11 publications

(4 citation statements)

References 95 publications

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“…Activated B lymphocytes are fused with HAT-sensitive myeloma cells using polyethylene glycol (PEG), which promotes the fusion of the plasma membranes. Electrofusion, using an electric field, is another method that is more efficient [77,78].…”

Section: Procedures Of Using Hybridoma To Produce Mabsmentioning

confidence: 99%

Production of Monoclonal Antibodies for Therapeutic Purposes: Applications, Techniques, and Improvement

Ding,

Huang

2024

Preprint

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Monoclonal antibodies (mAbs) have become an increasingly important therapeutic modality in recent years, with applications in oncology, hematology, and immunology. This review discusses the production of therapeutic mAbs, including the various types of mAbs (murine, chimeric, humanized, and fully human), their key applications, and the techniques used for their production, including hybridoma technology, single B cell antibody technology, and phage display technology. The review then proposes advanced techniques to overcome them. These include microfluidic sorting, alternative expression systems (including plants, bacteria, and yeast, and cell-free production systems), and the development of complex antibody formats like bi-specific monoclonal antibodies, antibody fragments, antibody-drug conjugates, and nanobody development. These advanced techniques promise to enhance the efficiency, cost-effectiveness, and scalability of mAb production, paving the way for more effective therapeutic applications. This review highlights the theoretical significance of integrating innovative technologies to overcome existing challenges in mAb production, ultimately advancing the field of precision medicine.

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Section: Procedures Of Using Hybridoma To Produce Mabsmentioning

confidence: 99%

Production of Monoclonal Antibodies for Therapeutic Purposes: Applications, Techniques, and Improvement

Ding,

Huang

2024

Preprint

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“…Another technique used for fusion is electrofusion, within which cells area unit consolidated beneath the result of an electrical field. This technique is additional deficient than the previous technique [76,77].…”

Section: Main Steps Of Hybridoma Technologymentioning

confidence: 99%

The Sars-Cov2 Epoch and Proper Managing Strategies to Face the Challenges Both in Viral Research and Treatment

Khaidarov

2023

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Пішімі 60х84/16. Көлемі 21,0 б.т. Тапсырыс № 7045. Әл-Фараби атындағы Қазақ ұлттық университетінің "Қазақ университеті" баспа үйі. 050040, Алматы қаласы, әл-Фараби даңғылы, 71. "Қазақ университеті" баспа үйінің баспаханасында басылды.

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“…The production of human monoclonal antibodies against predefined specificities has recently been achieved by hybridization of normal B cells to suitable mouse myelo ma, human/mouse hybridoma, human myeloma, or human lymphoblastoid cell lines and by direct transformation with Ep stein-Barr virus (EBY) [1,2]. We report in this communication a human anti-A red blood cell monoclonal antibody produced by EBV transformation of splenic B-lymphocytes after in vitro activation with group A red blood cells.…”

Section: Methodsmentioning

confidence: 99%

Human Monoclonal Antibodies against Group A Red Blood Cells

Raubitschek¹,

Senyk²,

Larrick³

et al. 1985

Vox Sanguinis

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A human monoclonal antibody with anti-A specificity was generated by Epstein-Barr virus transformation of lymphocytes isolated from splenic tissue after in vitro stimulation with group A red blood cells. This antibody is of the IgM class and directly agglutinates group A red blood cells. Type A(1), A(2), A(int), A3, A(x), A(end) and A(5) cells were agglutinated by the reagent indicating a single determinant is shared by these A subgroups.

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Production of Human Monoclonal Antibodies

Cited by 11 publications

References 95 publications

Production of Monoclonal Antibodies for Therapeutic Purposes: Applications, Techniques, and Improvement

Production of Monoclonal Antibodies for Therapeutic Purposes: Applications, Techniques, and Improvement

The Sars-Cov2 Epoch and Proper Managing Strategies to Face the Challenges Both in Viral Research and Treatment

Human Monoclonal Antibodies against Group A Red Blood Cells

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