Specific Heterologous Enhancement of Immune Responses

Rubin, Arnold S.; Coons, Albert H.

doi:10.1084/jem.135.2.437

Cited by 20 publications

(8 citation statements)

References 11 publications

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“…Therefore, it is possible that ethanol may inhibit macrophage as well as granulocyte production. Since macrophages may be involved in antigen processing (20), this, too, might contribute to suppression of immunocompetence in vitro.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Myelosuppression and Immunosuppression by Ethanol

Tisman¹,

Herbert²

1973

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

In Vitro Myelosuppression and Immunosuppression by Ethanol

Tisman¹,

Herbert²

1973

J. Clin. Invest.

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“…Specific stimulation in vitro of t h y m o c y t e s from T T -p r i m e d mice, cultured with S R B C -s t i m u l a t e d normal spleen cells, produced a significant increase in the a n t i -S R B C hemolysin response as measured b y plaque formation (14). In order to q u a n t i t a t e the n u m b e r of t h y m o c y t e s necessary to produce this amplifying effect in vitro, cell mixtures containing 10 million normal spleen cells and graded n u m b e r s of syngeneic thymocytes from mice immunized with TT were stimulated with SRBC and the specific priming antigen.…”

Section: Relative Numbers Of Primed Thymocytes or Spleen Cells Neededmentioning

confidence: 99%

“…Cultures.--In a previous paper we reported t h a t at least some components of antigen-specific immunological m e m o r y reside in the t h y m u s of mice immunized 1-2 m o n t h s earlier (14). Specific stimulation in vitro of t h y m o c y t e s from T T -p r i m e d mice, cultured with S R B C -s t i m u l a t e d normal spleen cells, produced a significant increase in the a n t i -S R B C hemolysin response as measured b y plaque formation (14).…”

Section: Relative Numbers Of Primed Thymocytes or Spleen Cells Neededmentioning

confidence: 99%

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Specific Heterologous Enhancement of Immune Responses

Rubin

Coons

1972

The Journal of Experimental Medicine

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In short-term cultures of thymocytes from tetanus toxoid-immunized mice, the addition of 1 ng of toxoid generated the release of a soluble factor which was capable of enhancing the immune response to a heterologous immunogen. The addition of supernatants from such cultures to assay cultures of sheep erythrocyte-stimulated normal spleen cells produced a significant augmentation of the hemolytic plaque response. Culture fluid from similar cultures of normal thymocytes or primed thymocytes cultured without the priming antigen were inactive. The enhancing factor was nondialyzable, heat stable (56°C, 30 min), resistant to DNAse and RNAse, but was inactivated by protease. A factor produced by specifically stimulated primed spleen cells had similar characteristics. In toxoid-stimulated, mixed cell cultures containing primed thymocytes or spleen cells and normal spleen cells, tenfold fewer thymocytes than spleen cells were needed to produce a comparable degree of enhancement of the anti-sheep erythrocyte plaque-forming cell response.

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“…Although these findings appear to establish a specific memory function of T cells, it should be realized that nonspecific enhancement of B cell function has also been accomplished by a variety of means in vivo (31)(32)(33)(34) and in vitro (33,(35)(36)(37)(38)(39). It is, therefore, important to study the reconstituting effect of various doses of cells sensitized to other antigens on the response of anti-0-treated SE-immune spleen cells.…”

Section: Complemen Tatio N Of a Nti-o-treated With A N Ti-i G-treatedmentioning

confidence: 99%

Surface Antigens of Immunocompetent Cells

Mond

Takahashi

Thorbecke

1972

The Journal of Experimental Medicine

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The effect of preincubation with anti-θ or anti-mouse immunoglobulin (Ig) and complement (C') on immune responsiveness of spleen cells from BALB/c mice immunized with sheep erythrocytes (SE) was investigated. Both treatments greatly depressed the remaining ability to produce a secondary response to SE in vitro. Normal BALB/c spleen cells were far less effective in reconstituting the responses of such depleted cell populations than were much smaller numbers of untreated immune spleen cells. Thymus-derived cell (T cell) memory appeared early after immunization and showed specificity for the immunizing antigens. Recombination of anti-Ig-treated with anti-θ-treated immune spleen cells resulted in virtually complete reconstitution of responsiveness. The presence of immunological memory in T cells and the nature of their surface receptors are discussed.

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Specific Heterologous Enhancement of Immune Responses

Cited by 20 publications

References 11 publications

In Vitro Myelosuppression and Immunosuppression by Ethanol

In Vitro Myelosuppression and Immunosuppression by Ethanol

Specific Heterologous Enhancement of Immune Responses

Surface Antigens of Immunocompetent Cells

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