Specific fluorescent detection of fibrillar α-synuclein using mono- and trimethine cyanine dyes

Volkova, Kateryna D.; Kovalska, Vladyslava; Balanda, A.O.; Losytskyy, Mykhaylo Yu.; Golub, Andriy G.; Vermeij, Rudolf J.; Subramaniam, Vinod; Tolmachev, OI; Yarmoluk, S. M.

doi:10.1016/j.bmc.2007.10.051

Cited by 64 publications

(40 citation statements)

References 15 publications

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“…4B). Fibril formation was studied using the thioflavin T (ThT) fluorescence assay, where an increase in ThT fluorescence intensity is considered indicative of this process [21,22]. Fluorescence variations of ThT for ASN in the absence and presence of PAMAM dendrimers are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

PAMAM G4 dendrimers affect the aggregation of α-synuclein

Miłowska¹,

Malachowska²,

Gabryelak³

2011

International Journal of Biological Macromolecules

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Section: Resultsmentioning

confidence: 99%

PAMAM G4 dendrimers affect the aggregation of α-synuclein

Miłowska¹,

Malachowska²,

Gabryelak³

2011

International Journal of Biological Macromolecules

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“…However, in the last decade, various molecular probes have been developed for detection of amyloid fibrils (Table 1). In many cases, various derivatives or related structures of both CR and ThT have been shown to be useful for in vitro and ex vivo monitoring of amyloid fibrils [63][64][65][66][67][68]. In addition, development of noninvasive methods to quantitate amyloid deposits is of great use in the diagnosis of amyloid diseases.…”

Section: Overview Of Molecular Probes For Amyloid Fibril Detectionmentioning

confidence: 98%

“…Radioactively labelled [55,56] PTAA Fluorescence [57] Resveratrol Fluorescence [58] SH-516 Fluorescence [59][60][61] T-284 Fluorescence [59,61] Thiazin red Fluorescence [62] Thioflavin S* Fluorescence [63,64] Thioflavin T Fluorescence [65,66] X-34 Fluorescence [67] *Mixture of planar asymmetric dyes with charged group at the end of the molecule.…”

Section: Overview Of Molecular Probes For Amyloid Fibril Detectionmentioning

confidence: 99%

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

2009

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Because understanding amyloid fibrillation in molecular detail is essential for development of strategies to control amyloid formation and overcome neurodegenerative disorders, increased understanding of present molecular probes as well as development of new probes are of utmost importance. To date, the binding modes of these molecular probes to amyloid fibrils are by no means adequately described or understood, and the large number of studies on Thioflavin T (ThT) and Congo Red (CR) binding have resulted in models that are incomplete and conflicting. Different types of binding sites are likely to be present in amyloid fibrils with differences in binding modes. ThT may bind in channels running parallel to the long axis of the fibril. In the channels, ThT may bind in either a monomeric or dimeric form of which the molecular conformation is likely to be planar. CR may bind in grooves formed along the β-sheets as a planar molecule in either a monomeric or supramolecular form.

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“…If the molecular dimension could insert into "binding channels" of BSA, the interaction would restrict dye molecules torsion/rotation, which resulted in fluorescence intensity enhancement. 18,19 The molecular dimension of Dye2 could preferentially insert into "binding channels" of BSA, so the fluorescence intensity of Dye2-BSA was stronger. Dye2 could be proposed as fluorescent dyes for BSA detection in aqueous solution.…”

Section: Spectral Properties Of the Dyes In The Presence Of Bsamentioning

confidence: 99%

Synthesis and Spectral Properties of Novel Water‐soluble Indocyanine Dyes as Fluorescent Dyes for Proteins Detection

Wang

Zhang

et al. 2011

Chin. J. Chem.

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Two novel water-soluble indocyanine dyes were synthesized. Their chemical structures were confirmed by 1 H NMR, IR, MS and UV-vis techniques. The absorptions of the prepared dyes in different solvents were measured. Two dyes exhibited negative solvatochromism, with a blue shift of the absorption and an emission maximum in (bovine serum albumin) protonic solvent relative to non protonic solvent. The fluorescence properties of the dyes in both free state and BSA were investigated. Significant enhancement of the fluorescence intensity for the Dye2 was observed in the presence of BSA. Dye2 could be proposed as fluorescent dyes for BSA detection in aqueous solution.

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Specific fluorescent detection of fibrillar α-synuclein using mono- and trimethine cyanine dyes

Cited by 64 publications

References 15 publications

PAMAM G4 dendrimers affect the aggregation of α-synuclein

PAMAM G4 dendrimers affect the aggregation of α-synuclein

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

Synthesis and Spectral Properties of Novel Water‐soluble Indocyanine Dyes as Fluorescent Dyes for Proteins Detection

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