Synthesis and Spectral Properties of Novel Water‐soluble Indocyanine Dyes as Fluorescent Dyes for Proteins Detection

Abstract

Two novel water-soluble indocyanine dyes were synthesized. Their chemical structures were confirmed by 1 H NMR, IR, MS and UV-vis techniques. The absorptions of the prepared dyes in different solvents were measured. Two dyes exhibited negative solvatochromism, with a blue shift of the absorption and an emission maximum in (bovine serum albumin) protonic solvent relative to non protonic solvent. The fluorescence properties of the dyes in both free state and BSA were investigated. Significant enhancement of the fluorescence intensity for the Dye2 was observed in the presence of BSA. Dye2 could be proposed as fluorescent dyes for BSA detection in aqueous solution.