Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina

Agaras, Betina Cecilia; Wall, Luis Gabriel; Valverde, Claudio

doi:10.1016/j.apsoil.2011.11.016

Cited by 22 publications

(32 citation statements)

References 57 publications

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“…In our experience (Agaras et al 2012 ), Gould's S1 medium has proven effective to recover a wide variety of pseudomonad isolates from soil and rhizosphere material, without signifi cant phylogenetic bias, and with a higher recovery and more colony morphotypes than the cetrimide agar medium commonly used in clinical laboratories for isolation of pseudomonads of clinical relevance. Both media are also differential because they induce siderophore production allowing distinction of fl uorescent from non-fl uorescent pseudomonad colonies.…”

Section: Isolating and Characterizing Pseudomonadsmentioning

confidence: 95%

“…16S rDNA sequencing confi rmed that 100 % of the colonies developed on Gould's S1 plates are positive for the oprF PCR and are members of the genus Pseudomonas (Agaras et al 2012 ). The typical protocol used in our lab is described below, and it may be subject to subtle adaptations depending on the available source of materials (Tables 21.3 and 21.4 ).…”

Section: Molecular Confi Rmation Of Isolatesmentioning

confidence: 98%

“…21.2.1 and 21.2.4 ). If both tests were positive, the endonuclease restriction pattern of the oprF amplicon may quickly allow distinguishing among two pseudomonad strains (Agaras et al 2012 ).…”

Section: Phase Variationmentioning

confidence: 98%

“…Isolated colonies from primary platings on Gould's S1 agar may serve as DNA source for a direct PCR detection procedure that is highly specifi c for the genus Pseudomonas , and that is based on the amplifi cation of an internal fragment of the oprF gene encoding an outer membrane porin (Agaras et al 2012 ). 16S rDNA sequencing confi rmed that 100 % of the colonies developed on Gould's S1 plates are positive for the oprF PCR and are members of the genus Pseudomonas (Agaras et al 2012 ).…”

Section: Molecular Confi Rmation Of Isolatesmentioning

confidence: 98%

“…21.1 ). The procedure described below (Agaras et al 2012 ) may be readily adapted to isolate Pseudomonas spp. from other sources like plant leaf, fruit, or grain surfaces.…”

Section: Isolation Of Pseudomonads From Soil and Plant Materialsmentioning

confidence: 99%

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Pseudomonas and Azospirillum

Valverde

Anta²,

Ferraris³

2015

Handbook for Azospirillum

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Pseudomonas strains are fast growing, genetically diverse and metabolically versatile bacteria. Many pseudomonad species are preferential inhabitants of the rhizosphere of plants, reaching up to 10 8 CFU/g of roots for crop species like soybean or maize in the fi eld. Rhizospheric pseudomonads contribute to plant growth and health through a variety of plant probiotic mechanisms, including protection of roots against fungal pathogen attack. Due to their relative ease to isolate and cultivate in the lab, there is an enormous wealth of knowledge about physiological, biochemical, and genetic traits of pseudomonads. Based on their PGPR traits, several inoculant products are commercialized, either for seed, foliar, or postharvest treatment of crops, vegetables, and fruits. Provided that pseudomonads share the rhizosphere niche with Azospirillum species, as well as with many other PGPR microorganisms, combined formulations have also become available for agronomic purposes. However, little information about interspecies and multispecies interactions is available. This chapter describes microbiological, genetic, and agronomic tools that may be applied to isolate and characterize novel Pseudomonas spp. from diverse source materials, to study their interaction with Azospirillum cells in the context of dual or multispecies inoculants, and to evaluate the quality and effectiveness of formulated products.

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Section: Isolating and Characterizing Pseudomonadsmentioning

confidence: 95%

Section: Molecular Confi Rmation Of Isolatesmentioning

confidence: 98%

Section: Phase Variationmentioning

confidence: 98%

Section: Molecular Confi Rmation Of Isolatesmentioning

confidence: 98%

“…21.1 ). The procedure described below (Agaras et al 2012 ) may be readily adapted to isolate Pseudomonas spp. from other sources like plant leaf, fruit, or grain surfaces.…”

Section: Isolation Of Pseudomonads From Soil and Plant Materialsmentioning

confidence: 99%

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