Specific detection of Echinococcus spp. from the Tibetan fox (Vulpes ferrilata) and the red fox (V. vulpes) using copro-DNA PCR analysis

Global Distribution of Alveolar and Cystic Echinococcosis

“…The Tibetan fox (V. ferrilata) has been described as an important definitive host in the eastern part of the Tibetan plateau [Qiu et al, 1999;cited in Vuitton et al (2003)]. In Sichuan province, E. multilocularis was detected in 35% of 94 faecal samples of Tibetan foxes collected in the environment (Jiang et al, 2012) and further east, in Qinghai province, 4 of 12 Tibetan foxes were infected . Evidence is accumulating that the Tibetan fox rather than the red fox is the more important definitive host of the Tibetan plateau (Tsukada et al, 2014).…”

Phylogenetic Pattern, Evolutionary Processes and Species Delimitation in the Genus Echinococcus

“…The two taxa occur sympatrically in the Tibetan plateau, with a partial, but not complete, separation of life cycles. Mixed infections of E. multilocularis and E. shiquicus have been found in the Tibetan fox (Vulpes ferrilata) (Jiang et al, 2012) and the plateau pika (Xiao et al, 2006). Importantly, differences in nuclear DNA sequences, as well as mtDNA sequences, have been found in strict sympatry, providing strong evidence for a lack of genetic exchangeability (Xiao et al, 2005).…”

Molecular detection of Taenia spp. in dogs' feces in Zanjan Province, Northwest of Iran

“…Despite post-mortem (necropsy) detection is highly sensitive and specific, it is evidently laborious, raises ethical issues, and is risky [10,11]. Two alternative approaches based on the detection of the parasitic copro-DNA molecules by polymerase chain reaction (PCR) and copro-antigens by enzyme-linked immunosorbent assay in animal fecal samples have been successfully developed and evaluated [12,13]. The detection and identification of copro-DNA may be advantageous over the detection of copro-antigens, as it provides the possibility of post-detection analysis of the sample DNA for precise identification of the proposed species.…”