Specific detection of avian influenza H5N2 whole virus particles on lateral flow strips using a pair of sandwich-type aptamers

Kim, Sang Hoon; Lee, Jun-Ho; Lee, Bang Hyun; Song, Chang Seon; Gu, Man Bock

doi:10.1016/j.bios.2019.03.061

Cited by 80 publications

(45 citation statements)

References 48 publications

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“…Detection of intact virus, i.e. whole virus particles (virions), is highly desirable for point-of-care testing platforms, such as LFBs 22,23 . Most approaches for nodavirus diagnosis are based on sample pretreatment for isolation and/or amplification of the target analyte, however the virion detection approach would enable virus detection without additional processing steps.…”

mentioning

confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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Effective analysis of pathogens causing human and veterinary diseases demands rapid, specific and sensitive detection methods which can be applied in research laboratory setups and in field for routine diagnosis. Paper lateral flow biosensors (LFBs) have been established as attractive tools for such analytical applications. In the present study a prototype LFB was designed for whole particles (virions) detection of nodavirus or fish nervous necrosis virus. Nodavirus is an important threat in the aquaculture industry, causing severe economic losses and environmental problems. The LFB was based on polyclonal antibodies conjugated on gold nanoparticles for signal visualization. Brain and retinas from fish samples were homogenized, centrifuged and the supernatant was directly applied on the LFB. formation of a red test line was indicative of nodavirus virions presence. nodavirus visual detection was completed in short time (30 min). Key factors of the LFB development influencing the assays' detection limit were characterized and the optimum parameters were determined, enabling increased efficiency, excluding non-specific interactions. Therefore, the proposed LFB assay consists a robust, simple, low cost and accurate method for detection of nodavirus virions in fish samples. The proposed biosensor is ideal for development of a commercial kit to be used on aquaculture facilities by fish farmers. It is anticipated that disease monitoring and environmental safety will benefit from the simplification of time consuming and costly procedures.Aquaculture is essential to cover fish-product demands, providing seafood in high quantities, and covering more than the half amount of fish consumed worldwide. This fact drives a strong demand for high production efficiency in aquaculture industry in order to cover the feeding needs of the world's growing population, in the middle of an increasing environmental crisis 1,2 . As a result, the aquaculture industry has continuously increased profits in a high rate. However, outbreaks of diseases caused by infectious agents are significantly restricting intensified aquaculture. According to literature 3 , 22.6% of all disease outbreaks are caused by viruses. Among these, viral nervous necrosis (VNN), also named vacuolating encephalopathy and retinopathy or encephalomyelitis, is a devastating disease, which induces cell necrosis accompanied by vacuolation in fish retina and brain. Its clinical symptoms include changes in skin color with abnormal swimming, low feed ingestion and altered buoyancy in affected fish. The disease is caused by nervous necrosis virus (NNV) or nodavirus, affecting more than 30 different fish species, worldwide. VNN causes high mortalities (80-100% in several species e.g. European sea bass), emerging as a major problem especially in the Mediterranean area, since it cannot be prevented by vaccination or effective treatment 4-6 .Fish nervous necrosis virus (belonging to Betanodavirus genus and Nodaviridae family) is icosahedral, and non-enveloped (∼25 nm in diameter). I...

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mentioning

confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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“…Another study used a pair of aptamers that specifically bound to avian influenza H5N2 viruses at multiple sites simultaneously [ 48 ]. This study was one of the few studies that used a homologous pair of aptamers to detect whole H5N2 virus particles instead of specific viral proteins, such as hemagglutinins.…”

Section: Noble Metal Nanoparticle-mediated Colorimetric Pathogen Detementioning

confidence: 99%

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Nguyen

Kim

2020

TrAC Trends in Analytical Chemistry

146

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The pervasive spread of infectious diseases and pandemics, such as the 2019 coronavirus disease (COVID-19), are becoming increasingly serious and urgent threats to human health. Preventing the spread of such diseases prioritizes the development of sensing devices that can rapidly, selectively, and reliably detect pathogens at minimal cost. Paper-based analytical devices (PADs) are promising tools that satisfy those criteria. Numerous paper-based biosensors have been established that rival conventional pathogen detection methods. Among them, colorimetric strategies are promising since results can be interpreted by eye, and are simple to operate, which is advantageous for point-of-care testing (POCT). Particularly, the application of nanomaterials on paper-based biosensors has become important as these materials are capable of converting signals from pathogens through unique mechanisms to yield an amplified colorimetric readout. To highlight the research progress on using nanomaterials in colorimetric paper-based biosensor for pathogen detection, we discuss the sensing mechanisms of how they work, structural and analytical characteristics of the devices, and representative recent applications. Current challenges and future directions of using PADs and nanomaterial-mediated strategies are also discussed.

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“…In other words, little research has been done on aptamerbased sandwich-type virus sensors. Kim's group recently developed immobilization-free SELEX and developed lateral flow strips that detect entire H5N2 virus particles using a pair of aptamers capable of binding to target molecules in a sandwich manner 79 . Figure 6(A) shows the graphene-oxide based immobilization-free screening method for developing an ssDNA aptamer capable of binding to the avian influenza virus particle.…”

Section: Optical-based Aptasensor For IV Detectionmentioning

confidence: 99%

Recent Development of Aptasensor for Influenza Virus Detection

Kim

Noh

et al. 2020

BioChip J

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In nowadays, we have entered the new era of pandemics and the significance of virus detection deeply impacts human society. Viruses with genetic mutations are reported nearly every year, and people have prepared tools to detect the virus and vaccines to ensure proper treatments. Influenza virus (IV) is one of the most harmful viruses reporting various mutations, sub-types, and rapid infection speed for humans and animals including swine and poultry. Moreover, IV infection presents several harmful symptoms including cough, fever, diarrhea, chills, even causing death. To reduce the IV-induced harm, its proper and rapid detection is highly required. Conventional techniques were used against various IV sub-types including H1N1, H3N2, and H5N1. However, some of the techniques are time-consuming, expensive, or labor-intensive for detecting IV. Recently, the nucleic acid-based aptamer has gained attention as a novel bioprobe for constructing a biosensor. In this review, the authors discuss the recent progress in aptasensors for detecting IV in terms of an electrochemical and an optical biosensor.

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Specific detection of avian influenza H5N2 whole virus particles on lateral flow strips using a pair of sandwich-type aptamers

Cited by 80 publications

References 48 publications

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Recent Development of Aptasensor for Influenza Virus Detection

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