Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton

Yamaguchi, Haruo; Yamaguchi, Mineo; Adachi, Masao

doi:10.3800/pbr.1.214

Cited by 6 publications

(4 citation statements)

References 17 publications

(10 reference statements)

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“…Phytoplankton incorporate P by direct assimilation of DIP (Cembella et al 1984, Chróst & Overbeck 1987, González-Gil et al 1998. In marine waters, the pool of DOP can comprise 25 to 80% of the dissolved P pool (Hoppe 2003, Suzumura et al 2012, though not all of it is biologically available (Karl & Bjorkman 2002), When DIP concentrations are depleted, phytoplankton may use reservoirs of DOP, like sugar phosphates and esters, for P deficiencies (Jackson & Williams 1985, Cotner & Wetzel 1992, Yamaguchi et al 2006. There is evidence to suggest that DOP compounds such as phosphomonoesters and less-labile phosphonates play an important role in phytoplankton (Dyhr man & Haley 2006, Dyhrman & Ruttenberg 2006, though the production of P-cleaving enzymes is more energetically costly than incorporation of DIP (Me seck et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Two methods are routinely used to determine APA in marine environments. The first, a bulk-assay enzyme method, where a specific phosphomonoester substrate is hydrolyzed in the presence of AP (Nicholson et al 2006), determines total APA (dissolved or particulate) for mixed assemblages (Koike & Nagata 1997, Li et al 1998) by measuring the hydrolyzed ester substrate in the water (González-Gil et al 1998, Yamaguchi et al 2006. It cannot readily distinguish species-specific contributions to APA (González-Gil et al 1998) and frequently overestimates phytoplankton APA by attributing heterotrophic bacterial APA, which is often greater than phytoplankton production, to the phytoplankton community (Sebastián et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Alkaline phosphatase activity detected in distinct phytoplankton communities in the northern Gulf of Alaska

Peacock¹,

Kudela²

2013

Mar. Ecol. Prog. Ser.

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Alkaline phosphatase activity (APA) has been proposed as a proxy to determine phosphorus (P) stress in marine phytoplankton. Phytoplankton typically meet P requirements by incorporating dissolved inorganic P (DIP), but can use reservoirs of dissolved organic P (DOP) by expressing alkaline phosphatase (AP). Enzyme-labeled fluorescence (ELF) was used to determine group-specific APA within 4 phytoplankton communities associated with mesoscale eddies, which are common to the northern Gulf of Alaska (nGoA). Nutrient stress in relation to eddies in the nGoA has not been well established and these anti-cyclonic eddies may be influencing P status in phytoplankton by isolating distinct biological communities. In the nGoA (fall 2007), APA was greatest in the northwest transitional waters of the Sitka eddy and the western edge of the Kenai eddy compared to coastal (shelf), eddy core waters, and open ocean waters. The APA signal was dominated by picoplankton (0.6 to 5 µm) and nanoplankton (5 to 20 µm), primarily nanoflagellates, though AP-producing phytoplankton were not the dominant phytoplankton at any station. APA did not correlate with DIP levels, and macronutrient concentrations suggest that DIP was not limiting for the bulk phytoplankton assemblage. Dissolved organic P (DOP) was not measured. These results suggest that P status within a community is more nuanced than typical Redfield or in organic nutrient indices indicate and that organic P sources could influence community composition of phytoplankton.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alkaline phosphatase activity detected in distinct phytoplankton communities in the northern Gulf of Alaska

Peacock¹,

Kudela²

2013

Mar. Ecol. Prog. Ser.

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“…Alkaline phosphatase activity was localized utilizing the ELF_97 Endogenous Phosphatase Detection Kit (E6601) (Thermo Fisher Scientific, Waltham USA) as described earlier [ 15 , 54 , 55 ]. Live cells from cultures where incubated with the alkaline phosphatase substrate.…”

Section: Methodsmentioning

confidence: 99%

Insights into the life strategy of the common marine diatom Chaetoceros peruvianus Brightwell

et al. 2018

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Chaetoceros peruvianus is a marine diatom species with circumglobal distribution. While frequently observed, it appears never to dominate the marine phytoplankton community hence it can be characterized as a rather opportunistic, generalistic species. Here we present ecological interpretations from a long-term data set on marine microphytoplankton in the northern Adriatic Sea, where the abundancies and relative contributions of C. peruvianus were observed along a set of steep ecological gradients. Limited supply of dissolved inorganic phosphate was identified as the driving ecological factor for this ecosystem. In parallel C. peruvianus was cultivated in monoclonal cultures and its morphological and physiological reaction to replete and phosphorus depleted medium was analysed. C. peruvianus reacted to phosphorus depletion by an increase in cell height and length as well as thickness and length of setae. This morphological reaction included an increase in cellular volume and calculated carbon content. Additionally, it represents the transition between two described morphological varieties, C. peruvianus and C. peruvianus var. robusta. C. peruvianus showed a significant induction of extracellular alkaline phosphatase activity if grown in phosphate depleted medium. Microscopical analysis demonstrated this activity to be located exclusively on the setae of the cells.

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“…APA is generally assumed to indicate a deficiency of bioavailable inorganic P and so cells attempt to utilize organically bound P (Cao et al, 2010;Sebastian et al, 2004). However, Yamaguchi et al (2006) found APA to be widely prevalent in coastal waters. The activity has been shown to increase when the orthophosphate concentration in the waters decreases to b 0.2 μM (Nausch, 1998).…”

Section: Introductionmentioning

confidence: 99%

Alkaline phosphatase activity at the southwest coast of India: A comparison of locations differently affected by upwelling

Mamatha

Malik

Varik

et al. 2015

Journal of Sea Research

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Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton

Cited by 6 publications

References 17 publications

Alkaline phosphatase activity detected in distinct phytoplankton communities in the northern Gulf of Alaska

Alkaline phosphatase activity detected in distinct phytoplankton communities in the northern Gulf of Alaska

Insights into the life strategy of the common marine diatom Chaetoceros peruvianus Brightwell

Alkaline phosphatase activity at the southwest coast of India: A comparison of locations differently affected by upwelling

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