Alkaline phosphatase activity at the southwest coast of India: A comparison of locations differently affected by upwelling

Mamatha, S.S.; Malik, Ashish; Varik, S.; Parvathi, Vallivattathillam; Jineesh, V.K.; Gauns, Mangesh; LokaBharathi, P.A.

doi:10.1016/j.seares.2014.06.002

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…AP activity is commonly used as an indicator for algal P-deficiency in natural aquatic ecosystems [ 43 , 44 , 45 ]. However, this study shows that AP activity also detects P-deficiency in laboratory algal species grown in real wastewater.…”

Section: Resultsmentioning

confidence: 99%

Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

et al. 2021

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Four microalgal species, Chlorella vulgaris, Botryococcus braunii, Ankistrodesmus falcatus, and Tetradesmus obliquus were studied for enhanced phosphorus removal from municipal wastewater after their exposure to phosphorus starvation. Microalgae were exposed to phosphorus starvation conditions for three and five days and then used in a batch experiment to purify an effluent from a small WWTP. After 3-day P-starvation, C. vulgaris biomass growth rate increased by 50% and its PO4 removal rate reached >99% within 7 days. B. braunii maintained good biomass growth rate and nutrient removal regardless of the P-starvation. All species showed 2–5 times higher alkaline phosphatase activity increase for P-starved biomass than at the reference conditions, responding to the decline of PO4 concentration in wastewater and biomass poly-P content. The overall efficiency of biomass P-starvation on enhanced phosphorus uptake was found to be dependent on the species, N/P molar ratio in the wastewater, as well as the biomass P content.

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Section: Resultsmentioning

confidence: 99%

Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

et al. 2021

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“…Four substrates have been used in the APA measurements, i.e., MUF-P 8,9,15-19,25-72 , DiFMUP 10,12,73-80 , MFP 13,[81][82][83][84][85] and pNPP [86][87][88][89][90][91][92][93][94] . Since a fluorescent (or colored) product is released when a substrate is hydrolysed by AP in a seawater sample, APA can be measured by detecting the changes in fluorescence (or color) over time.…”

Section: Methodsmentioning

confidence: 99%

A dataset of global ocean alkaline phosphatase activity

Song

Duhamel

et al. 2023

Sci Data

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Utilisation of dissolved organic phosphorus (DOP) by marine microbes as an alternative phosphorus (P) source when phosphate is scarce can help sustain non-Redfieldian carbon:nitrogen:phosphorus ratios and efficient ocean carbon export. However, global spatial patterns and rates of microbial DOP utilisation are poorly investigated. Alkaline phosphatase (AP) is an important enzyme group that facilitates the remineralisation of DOP to phosphate and thus its activity is a good proxy for DOP-utilisation, particularly in P-stressed regions. We present a Global Alkaline Phosphatase Activity Dataset (GAPAD) with 4083 measurements collected from 79 published manuscripts and one database. Measurements are organised into four groups based on substrate and further subdivided into seven size fractions based on filtration pore size. The dataset is globally distributed and covers major oceanic regions, with most measurements collected in the upper 20 m of low-latitude oceanic regions during summer since 1997. This dataset can help support future studies assessing global ocean P supply from DOP utilisation and provide a useful data reference for both field investigations and modelling activities.

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“…However, high rates of APA can occur in DIP-replete coastal waters and estuaries, which has been attributed to heterotrophic microbes breaking down organic matter to access carbon and nitrogen (Davis and Mahaffey, 2017;Labry et al, 2016;Nicholson et al, 2006). High APA rates were reported for coastal waters affected by upwelling and run-off from land off south-western India (0-3180 nmol l -1 h -1 , Mamatha et al, 2015), which were not correlated with DIP, however, the use of pNPP assay substrate at a much higher concentration (1 mM) precludes a direct comparison to our data. In their study of coastal waters around Malaysia, Lim et al (2018) reported a correlation between bacterial abundance and water column APA, and consequently attributed the APA bacterial alkaline phosphatases.…”

Section: Drivers Of Water Column Apamentioning

confidence: 99%

High rates of alkaline phosphatase activity in an equatorial coral reef ecosystem unrelated to seasonal nutrient dynamics

Nichols

Moynihan

Martin

2022

Preprint

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Coral reef productivity depends on fast nutrient cycling, mediated largely by enzymatic breakdown of organic matter. Alkaline phosphatases hydrolyse phosphomonoesters and are one of the key enzymes involved in marine phosphorus cycling. They are expressed by a plethora of marine organisms including both planktonic microbes and metazoans such as corals, often in response to phosphate limitation, and are potentially important for coral P nutrition and reef biogeochemical cycling. However, most alkaline phosphatase activity (APA) data are from open-ocean environments, and the rates and drivers of APA in coastal waters are not well understood. Here, we measured APA both in the water column and associated with three coral species at reefs in Singapore, where the monsoonal ocean current reversal creates strong seasonal changes in dissolved nutrient availability. Water column APA was consistently high, averaging 9 ± 10 nmol l-1 h-1, but was not correlated with dissolved phosphate or other biogeochemical parameters. Experimental phosphate addition did not reduce seawater APA but addition of labile organic carbon did increase seawater APA, indicating that the increase in APA was driven by heterotrophic activity rather than phosphate stress. Coral APA ranged from 12–163 µmol m-2 h-1 depending on species and was equivalent to the APA in several meters of overlying water. While most coral APA was associated with the coral holobiont rather than the coral mucus, corals released 12 – 55 µmol h-1 per m2 of mucus-associated APA into the water column, which is potentially significant for water column DOP cycling.

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Alkaline phosphatase activity at the southwest coast of India: A comparison of locations differently affected by upwelling

Cited by 9 publications

References 42 publications

Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

Increasing Phosphorus Uptake Efficiency by Phosphorus-Starved Microalgae for Municipal Wastewater Post-Treatment

A dataset of global ocean alkaline phosphatase activity

High rates of alkaline phosphatase activity in an equatorial coral reef ecosystem unrelated to seasonal nutrient dynamics

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