Specific connectivity between photoreceptors and horizontal cells in the zebrafish retina

Klaassen, Lauw J.; Graaff, Wim de; Asselt, Jorrit B. van; Klooster, Jan; Kamermans, Maarten

doi:10.1152/jn.00449.2016

Cited by 33 publications

(49 citation statements)

References 45 publications

(73 reference statements)

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“…HCs contact cones at specialised invaginations that are tightly sealed against the surrounding extracellular matrix (Chapot et al, 2017b;Regus-Leidig and Brandstätter, 2012) , meaning that their dendrites can act as highly specific and spatially restricted glutamate antennas (Chapot et al, 2017b). As a population, HCs contact all four types of cones in the zebrafish eye (Klaassen et al, 2016;Li et al, 2009;Yoshimatsu et al, 2014), meaning that only a subset of HC dendritic signals correspond to synaptic release from UVcones. To identify these contacts, we coexpressed mCherry in UV-cones ( Fig.…”

Section: Differentialmentioning

confidence: 99%

Cellular and molecular mechanisms of photoreceptor tuning for prey capture in larval zebrafish

Yoshimatsu

Schröder

et al. 2019

Preprint

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Correspondence to t. yoshimatsu@sussex.ac.uk and t.baden@sussex.ac.uk In the eye, the function of same-type photoreceptors must be regionally adjusted to process a highly asymmetrical natural visual world. Here we show that UV-cones in the larval zebrafish area temporalis are specifically tuned for UV-bright prey capture in their upper frontal visual field, which uses the signal from a single cone at a time. For this, UV-detection efficiency is regionally boosted 42-fold. Next, in vivo 2-photon imaging, transcriptomics and computational modelling reveal that these cones use an elevated baseline of synaptic calcium to facilitate the encoding of bright objects, which in turn results from expressional tuning of phototransduction genes. Finally, this signal is further accentuated at the level of glutamate release driving retinal networks. These regional differences tally with variations between peripheral and foveal cones in primates and hint at a common mechanistic origin. Together, our results highlight a rich mechanistic toolkit for the tuning of neurons.

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Section: Differentialmentioning

confidence: 99%

Cellular and molecular mechanisms of photoreceptor tuning for prey capture in larval zebrafish

Yoshimatsu

Schröder

et al. 2019

Preprint

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“…In zebrafish, the gap junction protein connexin55.5 is expressed in HCs. Although some studies propose that this marker is predominantly expressed in HA types of HCs (Godinho et al, 2007;Shields et al, 2007), a more recent study suggests that it is expressed in more diverse subtypes (Klaassen et al, 2016). The pancreas-specific transcription factor 1a (Ptf1a) is expressed in all HC subtypes in the zebrafish retina (Jusuf et al, 2011), while the TF thyroid hormone receptor β2 (trβ2) labels 70% of Ptf1a-positive HCs in the zebrafish retina (Suzuki et al, 2013).…”

Section: Horizontal Cell Labellingmentioning

confidence: 99%

Stochastic single cell migration leads to robust horizontal cell layer formation in the vertebrate retina

2019

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Developmental programs that arrange cells and tissues into patterned organs are remarkably robust. In the developing vertebrate retina, for example, neurons reproducibly assemble into distinct layers giving the mature organ its overall structured appearance. This stereotypic neuronal arrangement, termed lamination, is important for efficient neuronal connectivity. Although retinal lamination is conserved in many vertebrates, including humans, how it emerges from single cell behaviour is not fully understood. To shed light on this issue, we here investigated the formation of the retinal horizontal cell layer. Using in vivo light sheet imaging of the developing zebrafish retina, we generated a comprehensive quantitative analysis of horizontal single cell behaviour from birth to final positioning. Interestingly, we find that all parameters analysed, including cell cycle dynamics, migration paths and kinetics, as well as sister cell dispersal, are very heterogeneous. Thus, horizontal cells show individual non-stereotypic behaviour before final positioning. Yet these initially variable cell dynamics always generate the correct laminar pattern. Consequently, our data show that the extent of single cell stochasticity in the lamination of the vertebrate retina is underexplored.

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“…Third, most investigations into the function of zebrafish visual circuits have relied on long-wavelength light stimulation to limit interference with fluorescence imaging systems (Bollmann, 2019). However, like many surface-dwelling teleost fish (Baden and Osorio, 2019;Champ et al, 2016;Neumeyer, 1992), zebrafish have rich tetrachromatic colour vision (Krauss and Neumeyer, 2003;Meier et al, 2018;Orger and Baier, 2005), and spectrally diverse functions dominate both their outer (Klaassen et al, 2016) and inner retinal circuits (Connaughton and Nelson, 2010;Meier et al, 2018;Zimmermann et al, 2018). In fact, wavelength is strongly associated with specific behaviours in zebrafish.…”

Section: Introductionmentioning

confidence: 99%

What the Zebrafish’s Eye Tells the Zebrafish’s Brain: Retinal Ganglion Cells for Prey Capture and Colour Vision

Zhou

Bear

Roberts

et al. 2020

Preprint

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In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behaviour. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviours, comparatively little is known about what their eyes send to the brain in the first place via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding, and information on potentially critical variations in RGC properties across the retinal surface to acknowledge asymmetries in the statistics of natural visual space and behavioural demands.Here, we use in vivo two photon (2P) imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide the first eye-wide functional and anatomical census of RGCs in larval zebrafish.We find that RGCs' functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of colour opponency -long in excess of what would be required to satisfy traditional models of colour vision. However, most information on spectral contrast was intermixed with temporal information. To consolidate this series of unexpected findings, we propose that zebrafish may use a novel "dual-achromatic" strategy segregated by a spectrally intermediate background subtraction system. Specifically, our data is consistent with a model where traditional achromatic image-forming vision is mainly driven by longwavelength sensitive circuits, while in parallel UV-sensitive circuits serve a second achromatic system of foreground-vision that serves prey capture and, potentially, predator evasion.

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Specific connectivity between photoreceptors and horizontal cells in the zebrafish retina

Cited by 33 publications

References 45 publications

Cellular and molecular mechanisms of photoreceptor tuning for prey capture in larval zebrafish

Cellular and molecular mechanisms of photoreceptor tuning for prey capture in larval zebrafish

Stochastic single cell migration leads to robust horizontal cell layer formation in the vertebrate retina

What the Zebrafish’s Eye Tells the Zebrafish’s Brain: Retinal Ganglion Cells for Prey Capture and Colour Vision

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