Specific circulating immune complexes in amoebic liver abscess

Vinayak, V. K.; Purnima,; Singh, Kartar; Venkatwswarlu, K; Nain, C. K.; Mehta, S K

doi:10.1128/jcm.23.6.1088-1090.1986

Cited by 16 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results indicate that the TechLab antigen detection test can be used with serum samples to diagnose amebic liver abscess and can also be used as a test of cure. Detection of amebic antigen in serum of amebic liver abscess patients has been reported in the literature and has shown variable sensitivity and specificity (1,16,23,29). This is the first study to use a commercially available antigen detection test, and it has the advantage of the use of a well-defined antigen (the Gal/GalNAc lectin) (19,27).…”

Section: Discussionmentioning

confidence: 96%

Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

Haque

Mollah

Ali

et al. 2000

Journal of Clinical Microbiology

199

View full text Add to dashboard Cite

A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identifyEntamoeba histolytica in stool specimens from patients with amebic liver abscess. Here we report a method of detection in serum of circulating E. histolytica Gal/GalNAc lectin to diagnose amebic liver abscess, which was used in patients from Dhaka, Bangladesh. The TechLab E. histolytica II test (which differentiates the true pathogen E. histolytica fromEntamoeba dispar) detected Gal/GalNAc lectin in the sera of 22 of 23 (96%) amebic liver abscess patients tested prior to treatment with the antiamebic drug metronidazole and 0 of 70 (0%) controls. After 1 week of treatment with metronidazole, 9 of 11 (82%) patients became serum lectin antigen negative. The sensitivity of the E. histolytica II antigen detection test for intestinal infection was also evaluated. Antigen detection identified E. histolytica infection in 50 samples from 1,164 asymptomatic preschool children aged 2 to 5 years, including 16 of 16 (100%) culture-positive specimens. PCR analysis of stool specimens was used to confirm that most antigen-positive but culture-negative specimens were true-positive: PCR identified parasite DNA in 27 of 34 (79%) of the antigen-positive, culture-negative stool specimens. Antigen detection was a more sensitive test for infection than antilectin antibodies, which were detected in only 76 of 98 (78%) amebic liver abscess patients and in 26 of 50 (52%) patients with intestinal infection. We conclude that the TechLab E. histolytica II kit is a sensitive means to diagnose hepatic and intestinal amebiasis prior to the institution of metronidazole treatment.

show abstract

Section: Discussionmentioning

confidence: 96%

Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

Haque

Mollah

Ali

et al. 2000

Journal of Clinical Microbiology

199

View full text Add to dashboard Cite

show abstract

“…Detection of amebic antigen in serum has shown variable sensitivity and specificity in confirming the diagnosis of ALA. However, enzyme-linked immunosorbent and solid-phase radioimmunoassays have been reported to show sensitivities of 93% and 100%, respectively [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Pyogenic liver abscess in children

Duque-Estrada¹,

Duarte²,

Ribeiro³

2015

J Pediatr Infect Dis

View full text Add to dashboard Cite

Improvements in the medical and surgical care of pediatric patients have substantially reduced the incidence of pyogenic liver abscess, which is now a very uncommon condition in children. The purpose of this study was to review five cases of pyogenic liver abscess in children presenting to our hospital. Three patients were managed by percutaneous needle aspiration under ultrasonographic guidance together with antibiotic therapy. Two patients with multiple small abscesses were managed by prolonged antimicrobial treatment alone. All five patients survived. The etiology, diagnosis and medical and surgical management of liver abscess in children are discussed.

show abstract

“…Circulating immune complexes (CICs) in serum were precipitated by the polyethylene glycol (PEG) 6000 precipitation method [18].…”

Section: Precipitation Of Circulating Immune Complexesmentioning

confidence: 99%

Evaluation of integral membrane antigens of Mycobacterium habana for serodiagnosis of extrapulmonary tuberculosis: association between levels of antibodies and Mycobacterium tuberculosis antigens

Chaturvedi¹

2002

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

The performance of integral membrane antigens (IMAs) of Mycobacterium habana TMC 5135 in detecting antimycobacterial antibodies in serum and body fluids of patients mainly of extrapulmonary tuberculosis was evaluated. The IMAs were recovered from the detergent phase during Triton X-114 treatment of the plasma membrane of M. habana. Antimycobacterial antibodies were detected by ELISA using IMAs in serum and body fluids of 42 patients and 62 control subjects. As authentic adjunct Mycobacterium tuberculosis antigens were also detected (by ELISA) in body fluids and circulating immune complexes using anti-M. tuberculosis H37Ra antibodies. Anti-M. habana IMA antibody detection increased the positivity rate from 26.% (11/42) and 10% (4/42) obtained by culture and smear microscopy, respectively, to 86% (36/42). M. tuberculosis antigens were also found in 29 out of 36 anti-M. habana IMA antibody-positive cases. Interestingly, all 11 culture-positive cases were also positive for anti-M. habana IMA antibodies. The mean antigen titres in 23 cases, positive for antigens in body fluids, were 2.34 times higher in those who were also positive for anti-IMA antibodies in serum than in those negative for these antibodies. M. habana IMAs may be promising non-tubercular candidate antigens in ELISA-based serodiagnosis of extrapulmonary tuberculosis with substantial sensitivity, specificity and safety.

show abstract

Specific circulating immune complexes in amoebic liver abscess

Cited by 16 publications

References 13 publications

Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

Pyogenic liver abscess in children

Evaluation of integral membrane antigens of Mycobacterium habana for serodiagnosis of extrapulmonary tuberculosis: association between levels of antibodies and Mycobacterium tuberculosis antigens

Contact Info

Product

Resources

About