Specific capture of target proteins by oriented antibodies bound to tyrosinase-immobilized Protein A on a polyallylamine affinity membrane surface

Ahmed, Sufi R.; Lutes, Andrew T.; Barbari, Timothy A.

doi:10.1016/j.memsci.2006.05.033

Cited by 38 publications

(24 citation statements)

References 26 publications

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“…This strategy may use monoclonal or polyclonal antibodies, and permits an extremely selective protein adsorption, only the target protein becomes immobilized (i) if the antibody is properly immobilized (Ahmed et al, 2006, Batalla et al, 2008, Iwata et al, 2008, Schmid et al, 2006 and (ii) if we can prevent any undesired adsorption . Using polyclonal antibodies, the target protein will be immobilized following different orientations.…”

Section: Coupled Immobilization/purification Of Proteins Via Antibodymentioning

confidence: 99%

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

Barbosa

Ortíz

Berenguer-Murcia

et al. 2015

Biotechnology Advances

600

318

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Enzyme immobilization and purification are two steps that are usually required in the development of any industrial biocatalyst. In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of antibodies (versus the target enzyme or versus some domains), the development of specific domains with affinity for some specific supports or just to increase the affinity for standard ones (ionic exchangers, silicates) will be revised. We will show how the control of the immobilization conditions may convert some unspecific supports in largely specific ones. The development of tailormade heterofunctional supports as a tool to immobilize-stabilize-purify some proteins will be discussed in deep, using low concentration of adsorbents groups and a dense layer of groups able to give an intense multipoint covalent attachment. The final coupling of mutagenesis and tailor made supports will be a the last part of the review.

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Section: Coupled Immobilization/purification Of Proteins Via Antibodymentioning

confidence: 99%

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

Barbosa

Ortíz

Berenguer-Murcia

et al. 2015

Biotechnology Advances

600

318

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“…Another strategy, mainly used in biosensors development is based on the molecular mechanism that involve immobilized antibody-binding proteins, such as protein A or G that recognize the Fc fraction of antibody, orienting the Fb portions towards the mixture to be recognized leading to highly improved antigen detection [33].…”

Section: Succinimide Esters Activation 27mentioning

confidence: 99%

Molecular Recognition‐Driven Membrane Processes

Donato¹,

Mazzei²,

Algieri³

et al. 2014

Smart Membranes and Sensors

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High-precision, selective and effi cient systems are strongly demanded in all productive sectors, including chemical manufacturing, pharmaceutical, medical treatment, food, energy, diagnostic, analytical tools. Precision separation, conversion, formulation at molecular level may rely on various mechanisms and properties, such as molecular sieving, electrical charge, chemical solubility, molecular recognition. Membrane operation can promote mass transport, separation, conversion, formulation on the basis of the mentioned mechanisms. Biomimic and biohybrid funtionalised membranes are particularly suitable for separations based on molecular recognition.Membranes can be formed by polymers or monolythes bearing the (biomimic) recognition sites. In alternative, biomimic, biomolecules and recognition sites can be loaded on a previously formed membrane support. In such cases, surface functionalization methods can improve the loading effi ciency. Molecularly imprinted membranes; membranes bearing affi nity ligands (Antigen-Antibody, avidin/-ligand, DNA-protein, sugar-lectin, RNA-ribosome,…); membranes functionalized with zeolite and nanoparticles; membranes with immobilized enantioselective enzyme for kinetic resolutions, are among examples of selective and effi cient operations driven by molecular recognition. Th is is the mechanism by means of which molecules are able to identify each other using non-covalent -(hydrogen bonding, metal coordination, hydrophobic forces, van der Waals forces, π-π interactions and electrostatic eff ects) and covalent interactions.

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“…The applications of tyrosinase mediated cross-linking are wide-ranging. For example, tyrosinase mediated immobilisation of protein A has been used to greatly enhance the sensitivity of affinity membranes (Ahmed et al, 2006). Tyrosinase has also been used to modify the gel forming properties of chicken breast myofibrils .…”

Section: Introductionmentioning

confidence: 99%

Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase

Fairhead

Thöny‐Meyer

2010

Journal of Biotechnology

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Specific capture of target proteins by oriented antibodies bound to tyrosinase-immobilized Protein A on a polyallylamine affinity membrane surface

Cited by 38 publications

References 26 publications

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

Molecular Recognition‐Driven Membrane Processes

Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase

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