Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

Abstract

Enzyme immobilization and purification are two steps that are usually required in the development of any industrial biocatalyst. In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of antibodies (versus the target enzyme or versus some domains), the development of specific domains with affinity for some specific supports or just to increase the affinity for standard ones (ionic exchangers, silicates) will be revised. We will show how the control of the immobilization conditions may convert some unspecific supports in largely specific ones. The development of tailormade heterofunctional supports as a tool to immobilize-stabilize-purify some proteins will be discussed in deep, using low concentration of adsorbents groups and a dense layer of groups able to give an intense multipoint covalent attachment. The final coupling of mutagenesis and tailor made supports will be a the last part of the review.

A mild and efficient Dakin reaction mediated by lipase

“…It is known that the immobilized enzyme is easily recovered and reused, which can drive down the product cost and make the enzymatic process economically viable (26)(27)(28). Thus, we have studied the reusability of Novozym 435 for this lipase-mediated reaction.…”

A Novel Oxidation of Salicyl Alcohols Catalyzed by Lipase

“…As for the oxidant, we fixed the amount of UHP at 1.1 mmol due to the instability of salicylaldehyde exposed to excessive peracid [31]. Generally, the immobilized enzyme is easy to recover and reuse, which makes the enzymatic process economically viable [32][33][34]. In this work, Novozym 435 can be easily recovered after each run by filtration.…”

“…Generally, the immobilized enzyme is easy to recover and reuse, which makes the enzymatic process economically viable [32][33][34]. In this work, Novozym 435 can be easily recovered after each run by filtration.…”

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

“…Different types of surface functionalization and conditions for enzyme binding and coupling to the surface are evaluated. 2,10,22,30 The concept of the silica binding module aims at enzyme immobilization on silica by design [44][45][46] The design principle involves genetically encoded fusion of the silica-binding module to the enzyme of interest, thus conferring the ability to adsorb to plain silica surfaces to any enzyme in principle. A highly promising silica binding module is Z basic2.…”