Specific antibody levels and antigenic recognition of Wistar rats inoculated with distinct isolates of Trypanosoma evansi

Queiroz, Adriana O.; Legey, Ana Paula; Xavier, Samanta Cristina das Chagas; Jansen, Ana Maria

doi:10.1590/s0074-02762001000700014

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…The greater number of polypeptides detected in chronic phase of infection is probably due to the release of internal antigens after parasite destruction by variable surface glycoprotein (VSG) specific antibodies. Our results differ however from those of Queiroz et al (2001), who observed no variation in polypeptide profiles along the course of experimental infection in rats with Brazilian isolates of T. evansi.…”

Section: Discussioncontrasting

confidence: 99%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

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Section: Discussioncontrasting

confidence: 99%

“…Few studies on antigenic characterization of T. evansi have been undertaken (UCHE; JONES; BOID, 1993;JONES, 1994;QUEIROZ et al, 2001) and little is known so far regarding the polypeptide recognition patterns by antibodies from different host species.…”

Section: Introductionmentioning

confidence: 99%

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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“…In spite of the diversity of symptoms of the disease described in mammalian hosts, and of the complexity of pathogenesis in the different hosts species, T. evansi is a homogeneous taxon when evaluated by the several molecular tools used to characterize other trypanosomatids, such as RAPD, Zimodeme, and Schizodeme (Borst et al 1987;Songa et al 1990;Artama et al 1992;Queiroz et al 2001). Nevertheless, separation of chromosomal bands by pulsed field electrophoresis has been suggested as a promising method to discriminating T. evansi subpopulations from China (Lun et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Trypanosoma evansi in inbred and Swiss-Webster mice: distinct aspects of pathogenesis

et al. 2004

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Trypanosoma evansi (Trypanosomatidae, Kinetoplastida) is a salivarian trypanosomatid that infects eight mammal orders spread over America, Europe and Asia. In Brazil, T. evansi is the etiological agent of "Mal de Cadeiras", a horse disease very often described in the region known as Pantanal do Mato Grosso. Few data concerning the genetic diversity and biology of subpopulations of T. evansi that circulate in Brazil are available. The factors that modulate the interaction of this parasite with its hosts also remain to be elucidated. Here we evaluated the course of experimental infection of six T. evansi isolates derived from domestic and wild animals in Swiss-Webster mice and three Mus musculus lineages. The follow-up included biological, immunological as well as biochemical and hematological parameters. The same isolates as well as three others were characterized by pulsed-field electrophoresis. Our results showed that T. evansi isolates displayed significant differences regarding behavior and morbidity patterns in the distinct mouse lineages. Nevertheless, these differences could not be correlated with pulsed-field electrophoresis profiles. Indeed, concerning this molecular marker, only microheterogeneity was observed. Moreover, we observed that the outcome of the infection is defined by both host genetic background and peculiarities (virulence factors) of the distinct T. evansi isolates. Anemia and hypoglycemia were the only features that could be observed in all mouse lineages, independently of the inoculated T. evansi subpopulation. In addition, our data also show that Mus musculus is a suitable model host for the study of the different pathogenetic features of T. evansi infection.

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“…Acredita-se que essa diferença esteja relacionada ao processo de criopreservação, que retarda o início da multiplicação do flagelado, devido à mudança de ambiente ou hospedeiro (SILVA et al, 2003;SILVA et al, 2007). Em estudos anteriores, pesquisadores reportaram que o período pré-patente para a infecção por T. evansi, em ratos infectados pela via intraperitonial, pode variar entre 1,5 e 5,6 dias (OLIVEIRA et al, 1989;QUEIROZ et al, 2001;DOYLE et al, 2007), dependendo da dose infectante, diferentemente de QUEIROZ et al (2000), os quais não verificaram essa correlação referente à concentração de flagelado inoculada. Além disso, CARMONA et al (2006) relataram um período pré-patente de seis dias independentemente da cepa utilizada.…”

Section: Resultsunclassified

Patogenicidade de um isolado de Trypanosoma evansi em ratos inoculados com o parasito em sangue in natura e criopreservado

et al. 2009

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Specific antibody levels and antigenic recognition of Wistar rats inoculated with distinct isolates of Trypanosoma evansi

Cited by 11 publications

References 24 publications

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Trypanosoma evansi in inbred and Swiss-Webster mice: distinct aspects of pathogenesis

Patogenicidade de um isolado de Trypanosoma evansi em ratos inoculados com o parasito em sangue in natura e criopreservado

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