Specific and quantitative detection of human polyomaviruses BKV, JCV, and SV40 by real time PCR

Interleukin-2 Inhibits HIV-1 Replication in Some Human T Cell Lymphotrophic Virus-1-infected Cell Lines via the Induction and Incorporation of APOBEC3G into the Virion

“…Absolute numbers of copies of proviral DNA copy were determined at days 1, 2, 4, and 7 postinfection. To obtain absolute proviral DNA copy numbers, a standard curve was generated using serial dilutions of a plasmid encoding HIV gag region (pHIVgag) and RNaseP gene (pRNaseP) as described (47).…”

Interferon β1-a and selective anti-5HT2a receptor antagonists inhibit infection of human glial cells by JC virus

“…At 15 days post-infection virus was harvested from the infected cells, treated with Dnase to remove non-virion associated DNA, and then with proteinase K to expose the virion DNA. Virion associated DNA was then quantitated by real time PCR using the protocol, primers, and probes described by McNees et al on a Biorad IQ5 system (Biorad) (McNees et al, 2005). Primers and probes were designed using primer express software (Applied Biosystems).…”

Recipient seropositivity for adenovirus type 11 (AdV11) is a highly predictive factor for the development of AdV11-induced hemorrhagic cystitis after allogeneic hematopoietic SCT

“…Primers and probe for identification of all serotypes of AdV and those for identification of BKV were designed, based on the reports described previously. [4][5] As presented in Figure 1b, the urine AdV loads at the onset of HC in the 7 patients with AdV-HC were markedly higher than the maximum values in the 8 patients with non-AdV-HC or the 54 patients without HC. There was no significant difference in the serum AdV loads among the AdV-HC, non-AdV-HC and no HC group.…”