Specific and non-overlapping functions of testosterone and 11-ketotestosterone in the regulation of professional phagocyte responses in the teleost fish gilthead seabream

Águila, Sonia; Castillo-Briceño, Patricia; Sánchez, María Lidia; Cabas, Isabel; Garcı́a-Alcázar, A.; Meseguer, José; Mulero, Víctoriano; García‐Ayala, Alfonsa

doi:10.1016/j.molimm.2012.08.002

Cited by 23 publications

(19 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, in the last years, it has been described in the gilthead seabream that estrogens regulated the inflammatory immune response through endothelial cells and macrophage activation and increased leucocytes recruitment in the testis [ 51 – 54 ]. Furthermore, androgens such as testosterone also induced the recruitment of acidophilic granulocytes and IgM-positive cells in the testis of gilthead seabream and modulated in vitro the activity of gilthead seabream phagocytes and their sensitivity to pathogens [ 55 , 56 ]. On the other hand, increases on E 2 and T serum levels in the gilthead seabream, increased the complement and peroxidase activities at different time points although unmodified or decreased other humoral immune responses such as anti-protease activity and IgM serum levels [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

et al. 2015

View full text Add to dashboard Cite

Viruses are threatening pathogens for fish aquaculture. Some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (NNV). In order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. However, whether NNV colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. We have demonstrated for the first time the presence and localization of NNV into the testis after an experimental infection in the European sea bass (Dicentrarchus labrax), and in the gilthead seabream (Sparus aurata), a very susceptible and an asymptomatic host fish species, respectively. Thus, we localized in the testis viral RNA in both species using in situ PCR and viral proteins in gilthead seabream by immunohistochemistry, suggesting that males might also transmit the virus. In addition, we were able to isolate infective particles from the testis of both species demonstrating that NNV colonizes and replicates into the testis of both species. Blood contamination of the tissues sampled was discarded by completely fish bleeding, furthermore the in situ PCR and immunocytochemistry techniques never showed staining in blood vessels or cells. Moreover, we also determined how the immune and reproductive functions are affected comparing the effects in the testis with those found in the brain, the main target tissue of the virus. Interestingly, NNV triggered the immune response in the European sea bass but not in the gilthead seabream testis. Regarding reproductive functions, NNV infection alters 17β-estradiol and 11-ketotestosterone production and the potential sensitivity of brain and testis to these hormones, whereas there is no disruption of testicular functions according to several reproductive parameters. Moreover, we have also studied the NNV infection of the testis in vitro to assess local responses. Our in vitro results show that the changes observed on the expression of immune and reproductive genes in the testis of both species are different to those observed upon in vivo infections in most of the cases.

show abstract

Section: Discussionmentioning

confidence: 99%

Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Moreover, at 4 • C, the major impact on the leucocyte immune system was detected at 48 h of incubation along with an important cellular mortality starting from 24 h. In the manner, Misumi et al (2005) showed an important decrease of cellular viability after 17 h especially on splenic leucocytes. As an incubation time of 12-16 h is enough to determine the direct effects of EDCs on cells (Yao et al, 2007;Águila et al, 2013), a maximum incubation time of 12 h at 4 • C was suggesting in order to limit immune destabilization caused by leucocyte death. Second, it is important to study the eventual effects of the solvent on immune parameter tested.…”

Section: Discussionmentioning

confidence: 99%

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Bado‐Nilles¹,

Técher²,

Porcher³

et al. 2014

Environmental Toxicology and Pharmacology

View full text Add to dashboard Cite

Today, the list of endocrine disrupting compounds (EDCs) in freshwater and marine environments that mimic or block endogenous hormones is expanding at an alarming rate. As immune and reproductive systems may interact in a bidirectional way, some authors proposed the immune capacities as attractive markers to evaluate the hormonal potential of environmental samples. Thus, the present work proposed to gain more knowledge on direct biological effects of natural and EDCs on female fish splenic leucocyte non-specific immune activities by using ex vivo assays. After determining the optimal required conditions to analyze splenic immune responses, seven different EDCs were tested ex vivo at 0.01, 1 and 100nM over 12h on the leucocyte functions of female three-spined stickleback, Gasterosteus aculeatus. In summary, we found that natural hormones acted as immunostimulants, whilst EDCs were immunosuppressive.

show abstract

“…The three steroids induced a general transcriptional inhibition of sex steroid signalling receptors, which may be part of a mechanism to cope with excess of hormones and/or their derivatives, as suggested in other models [31,53]. Consistent with this view, in tadpole st 20–38, AR and ESR lowest expression levels occurred at the time of E2 and androgen highest peaks [32].…”

Section: Discussionmentioning

confidence: 63%

“…Differences between the responses driven by E2, T and EE2 could be due to multiple and distinct mechanisms, such as the regulation of specific sex steroid receptors, or the production of various metabolites resulting from sex steroids processing [31,53]. However, it is probable that the common responses, such as enhanced pigmentation, overgrown gills, increased cellular proliferation and induction of “non-typical” phenotypes could be mediated by a multifunctional receptor with similar affinity for all three sex hormones.…”

Section: Discussionmentioning

confidence: 99%

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Castillo-Briceño

Kodjabachian

2014

Front Zool

View full text Add to dashboard Cite

BackgroundHow important are sexual hormones beyond their function in reproductive biology has yet to be understood. In this study, we analyzed the effects of sex steroids on the biology of the embryonic amphibian epidermis, which represents an easily amenable model of non-reproductive mucociliary epithelia (MCE). MCE are integrated systems formed by multiciliated (MC), mucus-secreting (MS) and mitochondrion-rich (MR) cell populations that are shaped by their microenvironment. Therefore, MCE could be considered as ecosystems at the cellular scale, found in a wide array of contexts from mussel gills to mammalian oviduct.ResultsWe showed that the natural estrogen (estradiol, E2) and androgen (testosterone, T) as well as the synthetic estrogen (ethinyl-estradiol, EE2), all induced a significant enhancement of MC cell numbers. The effect of E2, T and EE2 extended to the MS and MR cell populations, to varying degrees. They also modified the expression profile of RNA MCE markers, and induced a range of “non-typical” cellular phenotypes, with mixed identities and aberrant morphologies, as revealed by imaging analysis through biomarker confocal detection and scanning electron microscopy. Finally, these hormones also affected tadpole pigmentation, revealing an effect on the entire cellular ecosystem of the Xenopus embryonic skin.ConclusionsThis study reveals the impact in vivo, at the molecular, cellular, tissue and organism levels, of sex steroids on non-reproductive mucociliary epithelium biogenesis, and validates the use of Xenopus as a relevant model system in this field.

show abstract

Specific and non-overlapping functions of testosterone and 11-ketotestosterone in the regulation of professional phagocyte responses in the teleost fish gilthead seabream

Cited by 23 publications

References 45 publications

Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Contact Info

Product

Resources

About