Specific and efficient electrochemical selection of Geoalkalibacter subterraneus and Desulfuromonas acetoxidans in high current-producing biofilms

Pierra, Mélanie; Carmona-Martínez, Alessandro A.; Trably, Eric; Godon, Jean‐Jacques; Bernet, Nicolas

doi:10.1016/j.bioelechem.2015.02.003

Cited by 43 publications

(35 citation statements)

References 34 publications

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“…S3 B0-B3). This finding is well in agreement with previous studies in which BES inoculated with sediments presented a high anodic microbial simplification (Pierra et al, 2015). On the contrary, several studies report a highly diverse microbial community with bacteria (ARB and non-ARB), especially for BES inoculated with non-saline sources such as domestic wastewater.…”

Section: Average Maximum Current Densitysupporting

confidence: 93%

“…Molecular analyses of bacterial communities were performed after acetate consumption in each biofilm reactor and enrichment steps. PCR products were separated by capillary electrophoresis single-strand conformation polymorphism (CE-SSCP), previously described (Pierra et al, 2015). With this technique, DNA fragments of the same size but with different DNA compositions are separated.…”

Section: Methodsmentioning

confidence: 99%

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Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria

Pierra

Carmona-Martínez

Trably

et al. 2015

Bioresource Technology

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Section: Average Maximum Current Densitysupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria

Pierra

Carmona-Martínez

Trably

et al. 2015

Bioresource Technology

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“…Desulfuromonas acetoxidans and ‘ Desulfuromonas soudanensis’ produce electric current using acetate [31, 32]. Desulfuromonas is frequently enriched in anodic biofilms using saline medium due to its halophilic characteristics [33], but is infrequently enriched with a conventional low-salt medium. Geobacter is a common exoelectrogenic genus that is enriched with low-salt medium.…”

Section: Resultsmentioning

confidence: 99%

Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates

et al. 2017

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BackgroundThe flame-oxidized stainless steel anode (FO-SSA) is a newly developed electrode that enhances microbial fuel cell (MFC) power generation; however, substrate preference and community structure of the biofilm developed on FO-SSA have not been well characterized. Herein, we investigated the community on FO-SSA using high-throughput sequencing of the 16S rRNA gene fragment in acetate-, starch-, glucose-, and livestock wastewater-fed MFCs. Furthermore, to analyze the effect of the anode material, the acetate-fed community formed on a common carbon-based electrode—carbon-cloth anode (CCA)—was examined for comparison.ResultsSubstrate type influenced the power output of MFCs using FO-SSA; the highest electricity was generated using acetate as a substrate, followed by peptone, starch and glucose, and wastewater. Intensity of power generation using FO-SSA was related to the abundance of exoelectrogenic genera, namely Geobacter and Desulfuromonas, of the phylum Proteobacteria, which were detected at a higher frequency in acetate-fed communities than in communities fed with other substrates. Lactic acid bacteria (LAB)—Enterococcus and Carnobacterium—were predominant in starch- and glucose-fed communities, respectively. In the wastewater-fed community, members of phylum Planctomycetes were frequently detected (36.2%). Exoelectrogenic genera Geobacter and Desulfuromonas were also detected in glucose-, starch-, and wastewater-fed communities on FO-SSA, but with low frequency (0–3.2%); the lactate produced by Carnobacterium and Enterococcus in glucose- and starch-fed communities might affect exoelectrogenic bacterial growth, resulting in low power output by MFCs fed with these substrates. Furthermore, in the acetate-fed community on FO-SSA, Desulfuromonas was abundant (15.4%) and Geobacter had a minor proportion (0.7%), while in that on CCA, both Geobacter and Desulfuromonas were observed at similar frequencies (6.0–9.8%), indicating that anode material affects exoelectrogenic genus enrichment in anodic biofilm.ConclusionsAnodic community structure was dependent on both substrate and anode material. Although Desulfuromonas spp. are marine microorganisms, they were abundant in the acetate-fed community on FO-SSA, implying the presence of novel non-halophilic and exoelectrogenic species in this genus. Power generation using FO-SSA was positively related to the frequency of exoelectrogenic genera in the anodic community. Predominant LAB in saccharide-fed anodic biofilm caused low abundance of exoelectrogenic genera and consequent low power generation.

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“…Extreme pH is particularly relevant for EET Wang et al (2012) used a U-shaped MFC to degrade petroleum hydrocarbon-contaminated saline soil, finding hydrocarbondegrading organisms in connected MFCs were 100 times more abundant than in open-circuit MFCs, highlighting that such devices can be used to achieve highly specialised enrichments. Pierra et al (2015) reported the enrichment of two EAM strains, Geoalkalibacter subterraneus and Desulfuromonas acetoxidans in saline sediments in potentiostat-controlled reactors operated at pH 7 and 5.5. Percentage domination of individual strains ranged from 86% to 99% depending on reactor, highlighting the quality of selection that can be achieved under highly controlled conditions.…”

Section: Extreme Environmentsmentioning

confidence: 99%

Methods for enrichment of novel electrochemically-active microorganisms

Doyle

Marsili

2015

Bioresource Technology

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Specific and efficient electrochemical selection of Geoalkalibacter subterraneus and Desulfuromonas acetoxidans in high current-producing biofilms

Cited by 43 publications

References 34 publications

Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria

Microbial characterization of anode-respiring bacteria within biofilms developed from cultures previously enriched in dissimilatory metal-reducing bacteria

Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates

Methods for enrichment of novel electrochemically-active microorganisms

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